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Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases

BACKGROUND: High loads of Epstein-Barr virus (EBV) in peripheral blood mononuclear cells (PBMCs) can be indicative of a broad spectrum of diseases, ranging from asymptomatic infection to fatal cancers. METHODS: We retrospectively investigated the EBV-infected cell types in PBMCs among 291 patients....

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Autores principales: Zhang, Peiling, Zeng, Chen, Cheng, Jiali, Zhou, Jing, Gu, Jia, Mao, Xia, Zhang, Wei, Cao, Yang, Luo, Hui, Xu, Bin, Li, Qinlu, Xiao, Min, Zhou, Jianfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499900/
https://www.ncbi.nlm.nih.gov/pubmed/31065564
http://dx.doi.org/10.1093/ofid/ofz171
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author Zhang, Peiling
Zeng, Chen
Cheng, Jiali
Zhou, Jing
Gu, Jia
Mao, Xia
Zhang, Wei
Cao, Yang
Luo, Hui
Xu, Bin
Li, Qinlu
Xiao, Min
Zhou, Jianfeng
author_facet Zhang, Peiling
Zeng, Chen
Cheng, Jiali
Zhou, Jing
Gu, Jia
Mao, Xia
Zhang, Wei
Cao, Yang
Luo, Hui
Xu, Bin
Li, Qinlu
Xiao, Min
Zhou, Jianfeng
author_sort Zhang, Peiling
collection PubMed
description BACKGROUND: High loads of Epstein-Barr virus (EBV) in peripheral blood mononuclear cells (PBMCs) can be indicative of a broad spectrum of diseases, ranging from asymptomatic infection to fatal cancers. METHODS: We retrospectively investigated the EBV-infected cell types in PBMCs among 291 patients. Based on EBV-infected cell types, the clinical features and prognoses of 93 patients with EBV-associated (EBV(+)) T/natural killer (NK)–cell lymphoproliferative diseases (LPDs) T/NK-LPDs) were investigated over a 5-year period. RESULTS: Although B-cell-type infection was found in immunocompromised patients and patients with asymptomatic high EBV carriage, infectious mononucleosis, EBV(+) B-cell LPDs and B-cell lymphomas, T-cell, NK-cell or multiple-cell-type infection in immunocompetent hosts were highly suggestive of EBV(+) T/NK-LPDs, EBV(+) T/NK-cell lymphomas, and aggressive NK-cell leukemia. Patients with non–B-cell infection had a poorer prognosis than those with B-cell-type infection. In our cohort, 79.6% of patients with EBV(+) T/NK-LPDs were >18 years old, and NK cells were identified as EBV-infected cell type in 54.8%. Nearly half of patients with EBV(+) T/NK-LPDs had genetic defects associated with immunodeficiency. However, hemophagocytic lymphohistiocytosis, and not genetic defects, was the only parameter correlated with poor prognosis of EBV(+) T/NK-LPDs. CONCLUSIONS: Determination of EBV-infected cell types among PBMCs is a valuable tool for the differential diagnosis of EBV(+) hematological diseases. In this study, determination of Epstein-Barr virus-infected cell types in peripheral blood mononuclear cells of 291 patients with high Epstein-Barr virus loads were retrospectively investigated, which indicate it is a valuable tool for Epstein-Barr virus-associated hematological diseases.
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spelling pubmed-64999002019-05-07 Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases Zhang, Peiling Zeng, Chen Cheng, Jiali Zhou, Jing Gu, Jia Mao, Xia Zhang, Wei Cao, Yang Luo, Hui Xu, Bin Li, Qinlu Xiao, Min Zhou, Jianfeng Open Forum Infect Dis Major Article BACKGROUND: High loads of Epstein-Barr virus (EBV) in peripheral blood mononuclear cells (PBMCs) can be indicative of a broad spectrum of diseases, ranging from asymptomatic infection to fatal cancers. METHODS: We retrospectively investigated the EBV-infected cell types in PBMCs among 291 patients. Based on EBV-infected cell types, the clinical features and prognoses of 93 patients with EBV-associated (EBV(+)) T/natural killer (NK)–cell lymphoproliferative diseases (LPDs) T/NK-LPDs) were investigated over a 5-year period. RESULTS: Although B-cell-type infection was found in immunocompromised patients and patients with asymptomatic high EBV carriage, infectious mononucleosis, EBV(+) B-cell LPDs and B-cell lymphomas, T-cell, NK-cell or multiple-cell-type infection in immunocompetent hosts were highly suggestive of EBV(+) T/NK-LPDs, EBV(+) T/NK-cell lymphomas, and aggressive NK-cell leukemia. Patients with non–B-cell infection had a poorer prognosis than those with B-cell-type infection. In our cohort, 79.6% of patients with EBV(+) T/NK-LPDs were >18 years old, and NK cells were identified as EBV-infected cell type in 54.8%. Nearly half of patients with EBV(+) T/NK-LPDs had genetic defects associated with immunodeficiency. However, hemophagocytic lymphohistiocytosis, and not genetic defects, was the only parameter correlated with poor prognosis of EBV(+) T/NK-LPDs. CONCLUSIONS: Determination of EBV-infected cell types among PBMCs is a valuable tool for the differential diagnosis of EBV(+) hematological diseases. In this study, determination of Epstein-Barr virus-infected cell types in peripheral blood mononuclear cells of 291 patients with high Epstein-Barr virus loads were retrospectively investigated, which indicate it is a valuable tool for Epstein-Barr virus-associated hematological diseases. Oxford University Press 2019-04-04 /pmc/articles/PMC6499900/ /pubmed/31065564 http://dx.doi.org/10.1093/ofid/ofz171 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Major Article
Zhang, Peiling
Zeng, Chen
Cheng, Jiali
Zhou, Jing
Gu, Jia
Mao, Xia
Zhang, Wei
Cao, Yang
Luo, Hui
Xu, Bin
Li, Qinlu
Xiao, Min
Zhou, Jianfeng
Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases
title Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases
title_full Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases
title_fullStr Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases
title_full_unstemmed Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases
title_short Determination of Epstein-Barr Virus–Infected Lymphocyte Cell Types in Peripheral Blood Mononuclear Cells as a Valuable Diagnostic Tool in Hematological Diseases
title_sort determination of epstein-barr virus–infected lymphocyte cell types in peripheral blood mononuclear cells as a valuable diagnostic tool in hematological diseases
topic Major Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499900/
https://www.ncbi.nlm.nih.gov/pubmed/31065564
http://dx.doi.org/10.1093/ofid/ofz171
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