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Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan

BACKGROUND: Over 60 years ago clinical patterns resembling tick-borne rickettsioses have been described for the first time in Kazakhstan. Since 1995 the incidence of clinical cases of tick-borne rickettsioses in humans seems to be rising but studies on epidemiological data regarding the occurring et...

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Autores principales: Turebekov, Nurkeldi, Abdiyeva, Karlygash, Yegemberdiyeva, Ravilya, Dmitrovsky, Andrey, Yeraliyeva, Lyazzat, Shapiyeva, Zhanna, Amirbekov, Aday, Oradova, Aksoltan, Kachiyeva, Zulfiya, Ziyadina, Lyazzat, Hoelscher, Michael, Froeschl, Guenter, Dobler, Gerhard, Zinner, Josua, Frey, Stefan, Essbauer, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500025/
https://www.ncbi.nlm.nih.gov/pubmed/31053085
http://dx.doi.org/10.1186/s13071-019-3440-9
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author Turebekov, Nurkeldi
Abdiyeva, Karlygash
Yegemberdiyeva, Ravilya
Dmitrovsky, Andrey
Yeraliyeva, Lyazzat
Shapiyeva, Zhanna
Amirbekov, Aday
Oradova, Aksoltan
Kachiyeva, Zulfiya
Ziyadina, Lyazzat
Hoelscher, Michael
Froeschl, Guenter
Dobler, Gerhard
Zinner, Josua
Frey, Stefan
Essbauer, Sandra
author_facet Turebekov, Nurkeldi
Abdiyeva, Karlygash
Yegemberdiyeva, Ravilya
Dmitrovsky, Andrey
Yeraliyeva, Lyazzat
Shapiyeva, Zhanna
Amirbekov, Aday
Oradova, Aksoltan
Kachiyeva, Zulfiya
Ziyadina, Lyazzat
Hoelscher, Michael
Froeschl, Guenter
Dobler, Gerhard
Zinner, Josua
Frey, Stefan
Essbauer, Sandra
author_sort Turebekov, Nurkeldi
collection PubMed
description BACKGROUND: Over 60 years ago clinical patterns resembling tick-borne rickettsioses have been described for the first time in Kazakhstan. Since 1995 the incidence of clinical cases of tick-borne rickettsioses in humans seems to be rising but studies on epidemiological data regarding the occurring etiological agents, tick vector species, prevalence and distribution throughout Kazakhstan are still scarce to date. The aim of the study was molecular investigation of ticks for spotted-fever group rickettsiae in the endemic Kyzylorda region and the so far considered as non-endemic Almaty region. A total of 2341 ticks was collected in the two regions in Kazakhstan and sorted in 501 pools: Ixodes persulcatus (243); Dermacentor marginatus (129); Haemaphysalis punctata (104); Hyalomma asiaticum (17); Dermacentor reticulatus (3); and Rhipicephalus turanicus (5). Pools were tested for Rickettsia spp. using real-time PCR. For positive samples multilocus sequence typing (MLST) was performed. RESULTS: The calculated minimum infection rate (MIR) for rickettsiae in the investigated ticks in Almaty region varied between 0.4–15.1% and 12.6–22.7% in the Kyzylorda region. At least four different Rickettsia species were identified in the two selected regions of Kazakhstan. Two of these are already known to science: Rickettsia raoultii and R. slovaca, the latter being reported for the first time in Almaty region One new form, “Candidatus R. yenbekshikazakhensis”, was described by MLST of six gene fragments in Almaty region and one new genotype, “genotype R. talgarensis” was detected using three gene fragments. CONCLUSIONS: Kazakh physicians should be aware of rickettsioses after tick bites in both regions studied. Both, R. raoultii and R. slovaca should be included in the diagnostics. The role for human diseases has further to be investigated for the newly described rickettsiae, “Candidatus R. yenbekshikazakhensis” and “Genotype R. talgarensis”.
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spelling pubmed-65000252019-05-09 Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan Turebekov, Nurkeldi Abdiyeva, Karlygash Yegemberdiyeva, Ravilya Dmitrovsky, Andrey Yeraliyeva, Lyazzat Shapiyeva, Zhanna Amirbekov, Aday Oradova, Aksoltan Kachiyeva, Zulfiya Ziyadina, Lyazzat Hoelscher, Michael Froeschl, Guenter Dobler, Gerhard Zinner, Josua Frey, Stefan Essbauer, Sandra Parasit Vectors Research BACKGROUND: Over 60 years ago clinical patterns resembling tick-borne rickettsioses have been described for the first time in Kazakhstan. Since 1995 the incidence of clinical cases of tick-borne rickettsioses in humans seems to be rising but studies on epidemiological data regarding the occurring etiological agents, tick vector species, prevalence and distribution throughout Kazakhstan are still scarce to date. The aim of the study was molecular investigation of ticks for spotted-fever group rickettsiae in the endemic Kyzylorda region and the so far considered as non-endemic Almaty region. A total of 2341 ticks was collected in the two regions in Kazakhstan and sorted in 501 pools: Ixodes persulcatus (243); Dermacentor marginatus (129); Haemaphysalis punctata (104); Hyalomma asiaticum (17); Dermacentor reticulatus (3); and Rhipicephalus turanicus (5). Pools were tested for Rickettsia spp. using real-time PCR. For positive samples multilocus sequence typing (MLST) was performed. RESULTS: The calculated minimum infection rate (MIR) for rickettsiae in the investigated ticks in Almaty region varied between 0.4–15.1% and 12.6–22.7% in the Kyzylorda region. At least four different Rickettsia species were identified in the two selected regions of Kazakhstan. Two of these are already known to science: Rickettsia raoultii and R. slovaca, the latter being reported for the first time in Almaty region One new form, “Candidatus R. yenbekshikazakhensis”, was described by MLST of six gene fragments in Almaty region and one new genotype, “genotype R. talgarensis” was detected using three gene fragments. CONCLUSIONS: Kazakh physicians should be aware of rickettsioses after tick bites in both regions studied. Both, R. raoultii and R. slovaca should be included in the diagnostics. The role for human diseases has further to be investigated for the newly described rickettsiae, “Candidatus R. yenbekshikazakhensis” and “Genotype R. talgarensis”. BioMed Central 2019-05-03 /pmc/articles/PMC6500025/ /pubmed/31053085 http://dx.doi.org/10.1186/s13071-019-3440-9 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Turebekov, Nurkeldi
Abdiyeva, Karlygash
Yegemberdiyeva, Ravilya
Dmitrovsky, Andrey
Yeraliyeva, Lyazzat
Shapiyeva, Zhanna
Amirbekov, Aday
Oradova, Aksoltan
Kachiyeva, Zulfiya
Ziyadina, Lyazzat
Hoelscher, Michael
Froeschl, Guenter
Dobler, Gerhard
Zinner, Josua
Frey, Stefan
Essbauer, Sandra
Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
title Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
title_full Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
title_fullStr Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
title_full_unstemmed Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
title_short Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
title_sort prevalence of rickettsia species in ticks including identification of unknown species in two regions in kazakhstan
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500025/
https://www.ncbi.nlm.nih.gov/pubmed/31053085
http://dx.doi.org/10.1186/s13071-019-3440-9
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