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Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops

KEY MESSAGE: Complex protein-containing reproductive secretions are a conserved trait amongst all extant gymnosperms; the pollination drops of most groups include carbohydrate-modifying enzymes and defence proteins. ABSTRACT: Pollination drops are aqueous secretions that receive pollen and transport...

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Autores principales: Prior, Natalie, Little, Stefan A., Boyes, Ian, Griffith, Patrick, Husby, Chad, Pirone-Davies, Cary, Stevenson, Dennis W., Tomlinson, P. Barry, von Aderkas, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500509/
https://www.ncbi.nlm.nih.gov/pubmed/30430247
http://dx.doi.org/10.1007/s00497-018-0348-z
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author Prior, Natalie
Little, Stefan A.
Boyes, Ian
Griffith, Patrick
Husby, Chad
Pirone-Davies, Cary
Stevenson, Dennis W.
Tomlinson, P. Barry
von Aderkas, Patrick
author_facet Prior, Natalie
Little, Stefan A.
Boyes, Ian
Griffith, Patrick
Husby, Chad
Pirone-Davies, Cary
Stevenson, Dennis W.
Tomlinson, P. Barry
von Aderkas, Patrick
author_sort Prior, Natalie
collection PubMed
description KEY MESSAGE: Complex protein-containing reproductive secretions are a conserved trait amongst all extant gymnosperms; the pollination drops of most groups include carbohydrate-modifying enzymes and defence proteins. ABSTRACT: Pollination drops are aqueous secretions that receive pollen and transport it to the ovule interior in gymnosperms (Coniferales, Cycadales, Ginkgoales, Gnetales). Proteins are well established as components of pollination drops in conifers (Coniferales) and Ephedra spp. (Gnetales), but it is unknown whether proteins are also present in the pollination drops of cycads (Cycadales), Ginkgo (Ginkgoales), Gnetum (Gnetales), or in the pollination drops produced by sterile ovules occurring on pollen plants in the Gnetales. We used liquid chromatography–tandem mass spectrometry followed by database-derived protein identification to conduct proteomic surveys of pollination drops collected from: Ceratozamia hildae, Zamia furfuracea and Cycas rumphii (Cycadales); Ginkgo biloba (Ginkgoales); Gnetum gnemon and Welwitschia mirabilis, including pollination drops from both microsporangiate and ovulate plants (Gnetales). We identified proteins in all samples: C. hildae (61), Z. furfuracea (40), C. rumphii (9), G. biloba (57), G. gnemon ovulate (17) and sterile ovules from microsporangiate plants (25) and W. mirabilis fertile ovules (1) and sterile ovules from microsporangiate plants (138). Proteins involved in defence and carbohydrate modification occurred in the drops of most groups, indicating conserved functions for proteins in pollination drops. Our study demonstrates that all extant gymnosperm groups produce complex reproductive secretions containing proteins, an ancient trait that likely contributed to the evolutionary success of seed plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00497-018-0348-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-65005092019-05-21 Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops Prior, Natalie Little, Stefan A. Boyes, Ian Griffith, Patrick Husby, Chad Pirone-Davies, Cary Stevenson, Dennis W. Tomlinson, P. Barry von Aderkas, Patrick Plant Reprod Original Article KEY MESSAGE: Complex protein-containing reproductive secretions are a conserved trait amongst all extant gymnosperms; the pollination drops of most groups include carbohydrate-modifying enzymes and defence proteins. ABSTRACT: Pollination drops are aqueous secretions that receive pollen and transport it to the ovule interior in gymnosperms (Coniferales, Cycadales, Ginkgoales, Gnetales). Proteins are well established as components of pollination drops in conifers (Coniferales) and Ephedra spp. (Gnetales), but it is unknown whether proteins are also present in the pollination drops of cycads (Cycadales), Ginkgo (Ginkgoales), Gnetum (Gnetales), or in the pollination drops produced by sterile ovules occurring on pollen plants in the Gnetales. We used liquid chromatography–tandem mass spectrometry followed by database-derived protein identification to conduct proteomic surveys of pollination drops collected from: Ceratozamia hildae, Zamia furfuracea and Cycas rumphii (Cycadales); Ginkgo biloba (Ginkgoales); Gnetum gnemon and Welwitschia mirabilis, including pollination drops from both microsporangiate and ovulate plants (Gnetales). We identified proteins in all samples: C. hildae (61), Z. furfuracea (40), C. rumphii (9), G. biloba (57), G. gnemon ovulate (17) and sterile ovules from microsporangiate plants (25) and W. mirabilis fertile ovules (1) and sterile ovules from microsporangiate plants (138). Proteins involved in defence and carbohydrate modification occurred in the drops of most groups, indicating conserved functions for proteins in pollination drops. Our study demonstrates that all extant gymnosperm groups produce complex reproductive secretions containing proteins, an ancient trait that likely contributed to the evolutionary success of seed plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00497-018-0348-z) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-11-14 2019 /pmc/articles/PMC6500509/ /pubmed/30430247 http://dx.doi.org/10.1007/s00497-018-0348-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Prior, Natalie
Little, Stefan A.
Boyes, Ian
Griffith, Patrick
Husby, Chad
Pirone-Davies, Cary
Stevenson, Dennis W.
Tomlinson, P. Barry
von Aderkas, Patrick
Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops
title Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops
title_full Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops
title_fullStr Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops
title_full_unstemmed Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops
title_short Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops
title_sort complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500509/
https://www.ncbi.nlm.nih.gov/pubmed/30430247
http://dx.doi.org/10.1007/s00497-018-0348-z
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