Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine

Mass spectrometer parameters such as Resolving Power, type of fragmentation, and mass calibration mode were optimized in the analysis of 24 pesticide metabolites in human urine using Ultra-High Pressure Liquid Chromatography coupled to Orbitrap High-Resolution Mass Spectrometer (UHPLC-HRMS). The bes...

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Autores principales: Dualde, Pablo, Coscollà, Clara, Pastor, Agustin, Yusà, Vicent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500652/
https://www.ncbi.nlm.nih.gov/pubmed/31118948
http://dx.doi.org/10.1155/2019/1917369
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author Dualde, Pablo
Coscollà, Clara
Pastor, Agustin
Yusà, Vicent
author_facet Dualde, Pablo
Coscollà, Clara
Pastor, Agustin
Yusà, Vicent
author_sort Dualde, Pablo
collection PubMed
description Mass spectrometer parameters such as Resolving Power, type of fragmentation, and mass calibration mode were optimized in the analysis of 24 pesticide metabolites in human urine using Ultra-High Pressure Liquid Chromatography coupled to Orbitrap High-Resolution Mass Spectrometer (UHPLC-HRMS). The best results were achieved with a Resolving Power of 25,000 FWHM and by applying Collision Induced Dissociation fragmentation mode (40 eV).
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spelling pubmed-65006522019-05-22 Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine Dualde, Pablo Coscollà, Clara Pastor, Agustin Yusà, Vicent Int J Anal Chem Research Article Mass spectrometer parameters such as Resolving Power, type of fragmentation, and mass calibration mode were optimized in the analysis of 24 pesticide metabolites in human urine using Ultra-High Pressure Liquid Chromatography coupled to Orbitrap High-Resolution Mass Spectrometer (UHPLC-HRMS). The best results were achieved with a Resolving Power of 25,000 FWHM and by applying Collision Induced Dissociation fragmentation mode (40 eV). Hindawi 2019-04-17 /pmc/articles/PMC6500652/ /pubmed/31118948 http://dx.doi.org/10.1155/2019/1917369 Text en Copyright © 2019 Pablo Dualde et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dualde, Pablo
Coscollà, Clara
Pastor, Agustin
Yusà, Vicent
Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine
title Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine
title_full Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine
title_fullStr Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine
title_full_unstemmed Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine
title_short Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine
title_sort optimization of resolving power, fragmentation, and mass calibration in an orbitrap spectrometer for analysis of 24 pesticide metabolites in urine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6500652/
https://www.ncbi.nlm.nih.gov/pubmed/31118948
http://dx.doi.org/10.1155/2019/1917369
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