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Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential
BACKGROUND: Lung cancer is a highly aggressive cancer with a poor prognosis and is associated with distant metastasis; however, there are no clinically recognized biomarkers for the early diagnosis and prediction of lung cancer metastasis. We sought to identify the differential mitochondrial protein...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6501018/ https://www.ncbi.nlm.nih.gov/pubmed/30950202 http://dx.doi.org/10.1111/1759-7714.13052 |
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author | Liu, Zhenkun Xu, Song Li, Lu Zhong, Xiaorong Chen, Chun Fan, Yaguang Shen, Wang Zu, Lingling Xue, Feng Wang, Min Zhou, Qinghua |
author_facet | Liu, Zhenkun Xu, Song Li, Lu Zhong, Xiaorong Chen, Chun Fan, Yaguang Shen, Wang Zu, Lingling Xue, Feng Wang, Min Zhou, Qinghua |
author_sort | Liu, Zhenkun |
collection | PubMed |
description | BACKGROUND: Lung cancer is a highly aggressive cancer with a poor prognosis and is associated with distant metastasis; however, there are no clinically recognized biomarkers for the early diagnosis and prediction of lung cancer metastasis. We sought to identify the differential mitochondrial protein profiles and understand the molecular mechanisms governing lung cancer metastasis. METHODS: Mitochondrial proteomic analysis was performed to screen and identify the differential mitochondrial protein profiles between human large cell lung cancer cell lines with high (L‐9981) and low (NL‐9980) metastatic potential by two‐dimensional differential gel electrophoresis. Western blot was used to validate the differential mitochondrial proteins from the two cells. Bioinformatic proteome analysis was performed using the Mascot search engine and messenger RNA expression of the 37 genes of the differential mitochondrial proteins were detected by real‐time PCR. RESULTS: Two hundred and seventeen mitochondrial proteins were differentially expressed between L‐9981 and NL‐9980 cells (P < 0.05). Sixty‐four analyzed proteins were identified by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry coupled with database interrogation. Ontology analysis revealed that these proteins were mainly involved in the regulation of translation, amino acid metabolism, tricarboxylic acid cycle, cancer invasion and metastasis, oxidative phosphorylation, intracellular signaling pathway, cell cycle, and apoptosis. CONCLUSION: Our results suggest that the incorporation of more samples and new datasets will permit the definition of a collection of proteins as potential biomarkers for the prediction and diagnosis of lung cancer metastasis. |
format | Online Article Text |
id | pubmed-6501018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-65010182019-05-10 Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential Liu, Zhenkun Xu, Song Li, Lu Zhong, Xiaorong Chen, Chun Fan, Yaguang Shen, Wang Zu, Lingling Xue, Feng Wang, Min Zhou, Qinghua Thorac Cancer Original Articles BACKGROUND: Lung cancer is a highly aggressive cancer with a poor prognosis and is associated with distant metastasis; however, there are no clinically recognized biomarkers for the early diagnosis and prediction of lung cancer metastasis. We sought to identify the differential mitochondrial protein profiles and understand the molecular mechanisms governing lung cancer metastasis. METHODS: Mitochondrial proteomic analysis was performed to screen and identify the differential mitochondrial protein profiles between human large cell lung cancer cell lines with high (L‐9981) and low (NL‐9980) metastatic potential by two‐dimensional differential gel electrophoresis. Western blot was used to validate the differential mitochondrial proteins from the two cells. Bioinformatic proteome analysis was performed using the Mascot search engine and messenger RNA expression of the 37 genes of the differential mitochondrial proteins were detected by real‐time PCR. RESULTS: Two hundred and seventeen mitochondrial proteins were differentially expressed between L‐9981 and NL‐9980 cells (P < 0.05). Sixty‐four analyzed proteins were identified by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry coupled with database interrogation. Ontology analysis revealed that these proteins were mainly involved in the regulation of translation, amino acid metabolism, tricarboxylic acid cycle, cancer invasion and metastasis, oxidative phosphorylation, intracellular signaling pathway, cell cycle, and apoptosis. CONCLUSION: Our results suggest that the incorporation of more samples and new datasets will permit the definition of a collection of proteins as potential biomarkers for the prediction and diagnosis of lung cancer metastasis. John Wiley & Sons Australia, Ltd 2019-04-04 2019-05 /pmc/articles/PMC6501018/ /pubmed/30950202 http://dx.doi.org/10.1111/1759-7714.13052 Text en © 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Liu, Zhenkun Xu, Song Li, Lu Zhong, Xiaorong Chen, Chun Fan, Yaguang Shen, Wang Zu, Lingling Xue, Feng Wang, Min Zhou, Qinghua Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential |
title | Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential |
title_full | Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential |
title_fullStr | Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential |
title_full_unstemmed | Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential |
title_short | Comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential |
title_sort | comparative mitochondrial proteomic analysis of human large cell lung cancer cell lines with different metastasis potential |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6501018/ https://www.ncbi.nlm.nih.gov/pubmed/30950202 http://dx.doi.org/10.1111/1759-7714.13052 |
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