Cargando…

To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway

BACKGROUND: Type 2 diabetes mellitus (T2DM) has become a chronic disease, serious harm to human health. Complications of the blood pipe are the main cause of disability and death in diabetic patients, including vascular lesions that directly affects the prognosis of patients with diabetes and surviv...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Jia-Rong, Qin, Xiu-Juan, Fang, Zhao-Hui, Li-Shan, Han, Li-Ping, Hui-Jiang, Guo, Ming-Fei, Jiang, Nan-Nan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6501128/
https://www.ncbi.nlm.nih.gov/pubmed/31179340
http://dx.doi.org/10.1155/2019/4650906
_version_ 1783416057890865152
author Gao, Jia-Rong
Qin, Xiu-Juan
Fang, Zhao-Hui
Li-Shan,
Han, Li-Ping
Hui-Jiang,
Guo, Ming-Fei
Jiang, Nan-Nan
author_facet Gao, Jia-Rong
Qin, Xiu-Juan
Fang, Zhao-Hui
Li-Shan,
Han, Li-Ping
Hui-Jiang,
Guo, Ming-Fei
Jiang, Nan-Nan
author_sort Gao, Jia-Rong
collection PubMed
description BACKGROUND: Type 2 diabetes mellitus (T2DM) has become a chronic disease, serious harm to human health. Complications of the blood pipe are the main cause of disability and death in diabetic patients, including vascular lesions that directly affects the prognosis of patients with diabetes and survival. This study was to determine the influence of high glucose and related mechanism of vascular lesion of type 2 diabetes mellitus pathogenesis. METHODS: In vivo aorta abdominalis of GK rats was observed with blood pressure, heart rate, hematoxylin and eosin (H&E), Masson, and Verhoeff staining. In vitro cells were cultured with 30 mM glucose for 24 h. RT-QPCR was used to detect the mRNA expression of endothelial markers PTEN, PI3K, Akt, and VEGF. Immunofluorescence staining was used to detect the expression of PTEN, PI3K, Akt, and VEGF. PI3K and Akt phosphorylation levels were detected by Western blot analysis. RESULTS: Heart rate, systolic blood pressure, diastolic blood pressure, and mean blood pressure in the GK control group were higher compared with the Wistar control group and no difference compared with the GK experimental model group. Fluorescence intensity of VEGF, Akt, and PI3K in the high-sugar stimulus group was stronger than the control group; PTEN in the high-sugar stimulus group was weakening than the control group. VEGF, Akt, and PI3K mRNA in the high-sugar stimulus group were higher than the control group; protein expressions of VEGF, Akt, and PI3K in the high-sugar stimulus group were higher than the control group. PTEN mRNA in the high-sugar stimulus group was lower than the control group. Protein expression of PTEN in the high-sugar stimulus group was lower than the control group. CONCLUSIONS: Angiogenesis is an important pathogenesis of T2DM vascular disease, and PTEN plays a negative regulatory role in the development of new blood vessels and can inhibit the PI3K/Akt signaling pathway.
format Online
Article
Text
id pubmed-6501128
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-65011282019-06-09 To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway Gao, Jia-Rong Qin, Xiu-Juan Fang, Zhao-Hui Li-Shan, Han, Li-Ping Hui-Jiang, Guo, Ming-Fei Jiang, Nan-Nan J Diabetes Res Research Article BACKGROUND: Type 2 diabetes mellitus (T2DM) has become a chronic disease, serious harm to human health. Complications of the blood pipe are the main cause of disability and death in diabetic patients, including vascular lesions that directly affects the prognosis of patients with diabetes and survival. This study was to determine the influence of high glucose and related mechanism of vascular lesion of type 2 diabetes mellitus pathogenesis. METHODS: In vivo aorta abdominalis of GK rats was observed with blood pressure, heart rate, hematoxylin and eosin (H&E), Masson, and Verhoeff staining. In vitro cells were cultured with 30 mM glucose for 24 h. RT-QPCR was used to detect the mRNA expression of endothelial markers PTEN, PI3K, Akt, and VEGF. Immunofluorescence staining was used to detect the expression of PTEN, PI3K, Akt, and VEGF. PI3K and Akt phosphorylation levels were detected by Western blot analysis. RESULTS: Heart rate, systolic blood pressure, diastolic blood pressure, and mean blood pressure in the GK control group were higher compared with the Wistar control group and no difference compared with the GK experimental model group. Fluorescence intensity of VEGF, Akt, and PI3K in the high-sugar stimulus group was stronger than the control group; PTEN in the high-sugar stimulus group was weakening than the control group. VEGF, Akt, and PI3K mRNA in the high-sugar stimulus group were higher than the control group; protein expressions of VEGF, Akt, and PI3K in the high-sugar stimulus group were higher than the control group. PTEN mRNA in the high-sugar stimulus group was lower than the control group. Protein expression of PTEN in the high-sugar stimulus group was lower than the control group. CONCLUSIONS: Angiogenesis is an important pathogenesis of T2DM vascular disease, and PTEN plays a negative regulatory role in the development of new blood vessels and can inhibit the PI3K/Akt signaling pathway. Hindawi 2019-04-17 /pmc/articles/PMC6501128/ /pubmed/31179340 http://dx.doi.org/10.1155/2019/4650906 Text en Copyright © 2019 Jia-Rong Gao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gao, Jia-Rong
Qin, Xiu-Juan
Fang, Zhao-Hui
Li-Shan,
Han, Li-Ping
Hui-Jiang,
Guo, Ming-Fei
Jiang, Nan-Nan
To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway
title To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway
title_full To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway
title_fullStr To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway
title_full_unstemmed To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway
title_short To Explore the Pathogenesis of Vascular Lesion of Type 2 Diabetes Mellitus Based on the PI3K/Akt Signaling Pathway
title_sort to explore the pathogenesis of vascular lesion of type 2 diabetes mellitus based on the pi3k/akt signaling pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6501128/
https://www.ncbi.nlm.nih.gov/pubmed/31179340
http://dx.doi.org/10.1155/2019/4650906
work_keys_str_mv AT gaojiarong toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway
AT qinxiujuan toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway
AT fangzhaohui toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway
AT lishan toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway
AT hanliping toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway
AT huijiang toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway
AT guomingfei toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway
AT jiangnannan toexplorethepathogenesisofvascularlesionoftype2diabetesmellitusbasedonthepi3kaktsignalingpathway