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Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments

We used an experimental approach of analyzing marine microcosms to evaluate the impact of both predation (top-down) and food resources (bottom-up) on spirotrich ciliate communities. To assess the diversity, we used two molecular methods–denaturing gradient gel electrophoresis (DGGE) and high-through...

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Autores principales: Grattepanche, Jean-David, Juarez, Doris L., Wood, Cameah C., McManus, George B., Katz, Laura A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502329/
https://www.ncbi.nlm.nih.gov/pubmed/31059530
http://dx.doi.org/10.1371/journal.pone.0215872
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author Grattepanche, Jean-David
Juarez, Doris L.
Wood, Cameah C.
McManus, George B.
Katz, Laura A.
author_facet Grattepanche, Jean-David
Juarez, Doris L.
Wood, Cameah C.
McManus, George B.
Katz, Laura A.
author_sort Grattepanche, Jean-David
collection PubMed
description We used an experimental approach of analyzing marine microcosms to evaluate the impact of both predation (top-down) and food resources (bottom-up) on spirotrich ciliate communities. To assess the diversity, we used two molecular methods–denaturing gradient gel electrophoresis (DGGE) and high-throughput sequencing (HTS). We carried out two types of experiments to measure top-down (adult copepods as predators) and bottom-up effects (phytoplankton as food resources) on the spirotrich ciliates. We observed both strong incubation effects (untreated controls departed from initial assessment of diversity) and high variability across replicates within treatments, particularly for the bottom-up experiments. This suggests a rapid community turn-over during incubation and differential susceptibility to the effects of experimental manipulation. Despite the variability, our analyses reveal some broad patterns such as (1) increasing adult copepod predator abundance had a greater impact on spirotrich ciliates than on other microbial eukaryotes; (2) there was no evidence for strong food selection by the dominant spirotrich ciliates.
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spelling pubmed-65023292019-05-23 Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments Grattepanche, Jean-David Juarez, Doris L. Wood, Cameah C. McManus, George B. Katz, Laura A. PLoS One Research Article We used an experimental approach of analyzing marine microcosms to evaluate the impact of both predation (top-down) and food resources (bottom-up) on spirotrich ciliate communities. To assess the diversity, we used two molecular methods–denaturing gradient gel electrophoresis (DGGE) and high-throughput sequencing (HTS). We carried out two types of experiments to measure top-down (adult copepods as predators) and bottom-up effects (phytoplankton as food resources) on the spirotrich ciliates. We observed both strong incubation effects (untreated controls departed from initial assessment of diversity) and high variability across replicates within treatments, particularly for the bottom-up experiments. This suggests a rapid community turn-over during incubation and differential susceptibility to the effects of experimental manipulation. Despite the variability, our analyses reveal some broad patterns such as (1) increasing adult copepod predator abundance had a greater impact on spirotrich ciliates than on other microbial eukaryotes; (2) there was no evidence for strong food selection by the dominant spirotrich ciliates. Public Library of Science 2019-05-06 /pmc/articles/PMC6502329/ /pubmed/31059530 http://dx.doi.org/10.1371/journal.pone.0215872 Text en © 2019 Grattepanche et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Grattepanche, Jean-David
Juarez, Doris L.
Wood, Cameah C.
McManus, George B.
Katz, Laura A.
Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments
title Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments
title_full Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments
title_fullStr Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments
title_full_unstemmed Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments
title_short Incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments
title_sort incubation and grazing effects on spirotrich ciliate diversity inferred from molecular analyses of microcosm experiments
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502329/
https://www.ncbi.nlm.nih.gov/pubmed/31059530
http://dx.doi.org/10.1371/journal.pone.0215872
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