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PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits

Icariin, the main effective component extracted from epimedium, has been shown to stimulate osteogenic differentiation and bone formation and to increase synthesis of the cartilage extracellular matrix. However, there has been little study on the effects of icariin on osteoarthritis. In this study,...

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Autores principales: Zhao, Chang Fu, Li, Zhen Hua, Li, Shao Jun, Li, Jian An, Hou, Ting Ting, Wang, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502375/
https://www.ncbi.nlm.nih.gov/pubmed/31183123
http://dx.doi.org/10.1098/rsos.181877
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author Zhao, Chang Fu
Li, Zhen Hua
Li, Shao Jun
Li, Jian An
Hou, Ting Ting
Wang, Yang
author_facet Zhao, Chang Fu
Li, Zhen Hua
Li, Shao Jun
Li, Jian An
Hou, Ting Ting
Wang, Yang
author_sort Zhao, Chang Fu
collection PubMed
description Icariin, the main effective component extracted from epimedium, has been shown to stimulate osteogenic differentiation and bone formation and to increase synthesis of the cartilage extracellular matrix. However, there has been little study on the effects of icariin on osteoarthritis. In this study, we loaded icariin onto poly(lactic-co-glycolic acid) (PLGA) electrospinning. The aim of this study was to explore a composite scaffold and to inhibit the progression of osteoarthritis. Our main experimental results demonstrated that the PLGA/icariin composite spinning scaffold had higher hydrophilicity, and icariin was released slowly and steadily from the scaffold. According to the results of an MTT test, immunofluorescence staining, an alkaline phosphate activating assay and a real-time polymerase chain reaction (RT-PCR) assay, the PLGA/icariin composite scaffold had good biocompatibility. In models of osteoarthritis, the results of a RT-PCR assay indicated that the PLGA/icariin scaffold promoted the synthesis of the extracellular matrix. The results of X-ray microtomography and histological evaluation demonstrated that the PLGA/icariin scaffold maintained the functional morphology of articular cartilage and inhibited the resorption of subchondral bone trabeculae. These findings indicated that the PLGA and icariin composite scaffold has therapeutic potential for use in the treatment of osteoarthritis.
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spelling pubmed-65023752019-06-10 PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits Zhao, Chang Fu Li, Zhen Hua Li, Shao Jun Li, Jian An Hou, Ting Ting Wang, Yang R Soc Open Sci Biology (Whole Organism) Icariin, the main effective component extracted from epimedium, has been shown to stimulate osteogenic differentiation and bone formation and to increase synthesis of the cartilage extracellular matrix. However, there has been little study on the effects of icariin on osteoarthritis. In this study, we loaded icariin onto poly(lactic-co-glycolic acid) (PLGA) electrospinning. The aim of this study was to explore a composite scaffold and to inhibit the progression of osteoarthritis. Our main experimental results demonstrated that the PLGA/icariin composite spinning scaffold had higher hydrophilicity, and icariin was released slowly and steadily from the scaffold. According to the results of an MTT test, immunofluorescence staining, an alkaline phosphate activating assay and a real-time polymerase chain reaction (RT-PCR) assay, the PLGA/icariin composite scaffold had good biocompatibility. In models of osteoarthritis, the results of a RT-PCR assay indicated that the PLGA/icariin scaffold promoted the synthesis of the extracellular matrix. The results of X-ray microtomography and histological evaluation demonstrated that the PLGA/icariin scaffold maintained the functional morphology of articular cartilage and inhibited the resorption of subchondral bone trabeculae. These findings indicated that the PLGA and icariin composite scaffold has therapeutic potential for use in the treatment of osteoarthritis. The Royal Society 2019-04-10 /pmc/articles/PMC6502375/ /pubmed/31183123 http://dx.doi.org/10.1098/rsos.181877 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Biology (Whole Organism)
Zhao, Chang Fu
Li, Zhen Hua
Li, Shao Jun
Li, Jian An
Hou, Ting Ting
Wang, Yang
PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits
title PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits
title_full PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits
title_fullStr PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits
title_full_unstemmed PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits
title_short PLGA scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits
title_sort plga scaffold carrying icariin to inhibit the progression of osteoarthritis in rabbits
topic Biology (Whole Organism)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502375/
https://www.ncbi.nlm.nih.gov/pubmed/31183123
http://dx.doi.org/10.1098/rsos.181877
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