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Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus
Two eye-colour mutant strains, white (W) and yellow (Y) of house cricket Acheta domesticus were established in our laboratory. We phenotyped and genotyped the mutants, performed genetic crossings and studied the eye structure and pigment composition using light and electron microscopy and biochemica...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502451/ https://www.ncbi.nlm.nih.gov/pubmed/31059541 http://dx.doi.org/10.1371/journal.pone.0216281 |
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author | Francikowski, Jacek Krzyżowski, Michał Kochańska, Barbara Potrzebska, Marta Baran, Bartosz Chajec, Łukasz Urbisz, Anna Małota, Karol Łozowski, Bartosz Kloc, Malgorzata Kubiak, Jacek |
author_facet | Francikowski, Jacek Krzyżowski, Michał Kochańska, Barbara Potrzebska, Marta Baran, Bartosz Chajec, Łukasz Urbisz, Anna Małota, Karol Łozowski, Bartosz Kloc, Malgorzata Kubiak, Jacek |
author_sort | Francikowski, Jacek |
collection | PubMed |
description | Two eye-colour mutant strains, white (W) and yellow (Y) of house cricket Acheta domesticus were established in our laboratory. We phenotyped and genotyped the mutants, performed genetic crossings and studied the eye structure and pigment composition using light and electron microscopy and biochemical analysis. We show that W and Y phenotypes are controlled by a single autosomal recessive allele, as both traits are metabolically independent. The analysis of the mutants`eye structure showed a reduced number of dark pigment granules while simultaneously, and an increased amount of light vacuoles in white eye mutants was observed. Significant differences in eye pigment composition between strains were also found. The Y mutant had a lower number of ommochromes, while the W mutant had a lower number of ommochromes and pteridines. This indicates that mutated genes are involved in two different, independent metabolic pathways regulating tryptophan metabolism enzymes, pigment transporter granules or pigment granule formation. |
format | Online Article Text |
id | pubmed-6502451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65024512019-05-23 Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus Francikowski, Jacek Krzyżowski, Michał Kochańska, Barbara Potrzebska, Marta Baran, Bartosz Chajec, Łukasz Urbisz, Anna Małota, Karol Łozowski, Bartosz Kloc, Malgorzata Kubiak, Jacek PLoS One Research Article Two eye-colour mutant strains, white (W) and yellow (Y) of house cricket Acheta domesticus were established in our laboratory. We phenotyped and genotyped the mutants, performed genetic crossings and studied the eye structure and pigment composition using light and electron microscopy and biochemical analysis. We show that W and Y phenotypes are controlled by a single autosomal recessive allele, as both traits are metabolically independent. The analysis of the mutants`eye structure showed a reduced number of dark pigment granules while simultaneously, and an increased amount of light vacuoles in white eye mutants was observed. Significant differences in eye pigment composition between strains were also found. The Y mutant had a lower number of ommochromes, while the W mutant had a lower number of ommochromes and pteridines. This indicates that mutated genes are involved in two different, independent metabolic pathways regulating tryptophan metabolism enzymes, pigment transporter granules or pigment granule formation. Public Library of Science 2019-05-06 /pmc/articles/PMC6502451/ /pubmed/31059541 http://dx.doi.org/10.1371/journal.pone.0216281 Text en © 2019 Francikowski et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Francikowski, Jacek Krzyżowski, Michał Kochańska, Barbara Potrzebska, Marta Baran, Bartosz Chajec, Łukasz Urbisz, Anna Małota, Karol Łozowski, Bartosz Kloc, Malgorzata Kubiak, Jacek Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus |
title | Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus |
title_full | Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus |
title_fullStr | Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus |
title_full_unstemmed | Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus |
title_short | Characterisation of white and yellow eye colour mutant strains of house cricket, Acheta domesticus |
title_sort | characterisation of white and yellow eye colour mutant strains of house cricket, acheta domesticus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502451/ https://www.ncbi.nlm.nih.gov/pubmed/31059541 http://dx.doi.org/10.1371/journal.pone.0216281 |
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