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Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi

High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolut...

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Autores principales: Lamien-Meda, Aline, Fuehrer, Hans-Peter, Noedl, Harald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502744/
https://www.ncbi.nlm.nih.gov/pubmed/31080854
http://dx.doi.org/10.1016/j.dib.2019.103937
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author Lamien-Meda, Aline
Fuehrer, Hans-Peter
Noedl, Harald
author_facet Lamien-Meda, Aline
Fuehrer, Hans-Peter
Noedl, Harald
author_sort Lamien-Meda, Aline
collection PubMed
description High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolution melting profiles of P. ovale curtisi (Poc) and P. ovale wallikeri (Pow) in the following conditions: after a direct qPCR run and after a nested snapback run. The qPCR-HRM of artificial mixture of Poc and Pow plasmids (200 copies/μl, each) at different proportions are showing the melting pattern of co-infections with both species. The sequencing methodology of the clpc gene fragment of 12 randomly selected samples is described and their likeness to published sequences is shown in a maximum likelihood tree. “Novel high resolution melting and snapback assays for simultaneous detection and differentiation of Plamodium ovale spp.” [1].
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spelling pubmed-65027442019-05-10 Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi Lamien-Meda, Aline Fuehrer, Hans-Peter Noedl, Harald Data Brief Biochemistry, Genetics and Molecular Biology High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolution melting profiles of P. ovale curtisi (Poc) and P. ovale wallikeri (Pow) in the following conditions: after a direct qPCR run and after a nested snapback run. The qPCR-HRM of artificial mixture of Poc and Pow plasmids (200 copies/μl, each) at different proportions are showing the melting pattern of co-infections with both species. The sequencing methodology of the clpc gene fragment of 12 randomly selected samples is described and their likeness to published sequences is shown in a maximum likelihood tree. “Novel high resolution melting and snapback assays for simultaneous detection and differentiation of Plamodium ovale spp.” [1]. Elsevier 2019-04-23 /pmc/articles/PMC6502744/ /pubmed/31080854 http://dx.doi.org/10.1016/j.dib.2019.103937 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Lamien-Meda, Aline
Fuehrer, Hans-Peter
Noedl, Harald
Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
title Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
title_full Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
title_fullStr Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
title_full_unstemmed Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
title_short Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
title_sort data on high resolution melting (hrm) and phylogenetic analysis of p. ovale wallikeri and p. ovale curtisi
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502744/
https://www.ncbi.nlm.nih.gov/pubmed/31080854
http://dx.doi.org/10.1016/j.dib.2019.103937
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