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Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi
High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolut...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502744/ https://www.ncbi.nlm.nih.gov/pubmed/31080854 http://dx.doi.org/10.1016/j.dib.2019.103937 |
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author | Lamien-Meda, Aline Fuehrer, Hans-Peter Noedl, Harald |
author_facet | Lamien-Meda, Aline Fuehrer, Hans-Peter Noedl, Harald |
author_sort | Lamien-Meda, Aline |
collection | PubMed |
description | High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolution melting profiles of P. ovale curtisi (Poc) and P. ovale wallikeri (Pow) in the following conditions: after a direct qPCR run and after a nested snapback run. The qPCR-HRM of artificial mixture of Poc and Pow plasmids (200 copies/μl, each) at different proportions are showing the melting pattern of co-infections with both species. The sequencing methodology of the clpc gene fragment of 12 randomly selected samples is described and their likeness to published sequences is shown in a maximum likelihood tree. “Novel high resolution melting and snapback assays for simultaneous detection and differentiation of Plamodium ovale spp.” [1]. |
format | Online Article Text |
id | pubmed-6502744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-65027442019-05-10 Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi Lamien-Meda, Aline Fuehrer, Hans-Peter Noedl, Harald Data Brief Biochemistry, Genetics and Molecular Biology High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolution melting profiles of P. ovale curtisi (Poc) and P. ovale wallikeri (Pow) in the following conditions: after a direct qPCR run and after a nested snapback run. The qPCR-HRM of artificial mixture of Poc and Pow plasmids (200 copies/μl, each) at different proportions are showing the melting pattern of co-infections with both species. The sequencing methodology of the clpc gene fragment of 12 randomly selected samples is described and their likeness to published sequences is shown in a maximum likelihood tree. “Novel high resolution melting and snapback assays for simultaneous detection and differentiation of Plamodium ovale spp.” [1]. Elsevier 2019-04-23 /pmc/articles/PMC6502744/ /pubmed/31080854 http://dx.doi.org/10.1016/j.dib.2019.103937 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Lamien-Meda, Aline Fuehrer, Hans-Peter Noedl, Harald Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi |
title | Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi |
title_full | Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi |
title_fullStr | Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi |
title_full_unstemmed | Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi |
title_short | Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi |
title_sort | data on high resolution melting (hrm) and phylogenetic analysis of p. ovale wallikeri and p. ovale curtisi |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502744/ https://www.ncbi.nlm.nih.gov/pubmed/31080854 http://dx.doi.org/10.1016/j.dib.2019.103937 |
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