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Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation
The physiologic response to tobacco smoke can be measured by gene-expression profiling of the airway epithelium. Temporal resolution of kinetics of gene-expression alterations upon smoking-cessation might delineate distinct biological processes that are activated during recovery from tobacco smoke e...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502805/ https://www.ncbi.nlm.nih.gov/pubmed/31061400 http://dx.doi.org/10.1038/s41598-019-43295-3 |
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author | Hijazi, Kahkeshan Malyszko, Bozena Steiling, Katrina Xiao, Xiaohui Liu, Gang Alekseyev, Yuriy O. Dumas, Yves-Martine Hertsgaard, Louise Jensen, Joni Hatsukami, Dorothy Brooks, Daniel R. O’Connor, George Beane, Jennifer Lenburg, Marc E. Spira, Avrum |
author_facet | Hijazi, Kahkeshan Malyszko, Bozena Steiling, Katrina Xiao, Xiaohui Liu, Gang Alekseyev, Yuriy O. Dumas, Yves-Martine Hertsgaard, Louise Jensen, Joni Hatsukami, Dorothy Brooks, Daniel R. O’Connor, George Beane, Jennifer Lenburg, Marc E. Spira, Avrum |
author_sort | Hijazi, Kahkeshan |
collection | PubMed |
description | The physiologic response to tobacco smoke can be measured by gene-expression profiling of the airway epithelium. Temporal resolution of kinetics of gene-expression alterations upon smoking-cessation might delineate distinct biological processes that are activated during recovery from tobacco smoke exposure. Using whole genome gene-expression profiling of individuals initiating a smoking-cessation attempt, we sought to characterize the kinetics of gene-expression alterations in response to short-term smoking-cessation in the nasal epithelium. RNA was extracted from the nasal epithelial of active smokers at baseline and at 4, 8, 16, and 24-weeks after smoking-cessation and put onto Gene ST arrays. Gene-expression levels of 119 genes were associated with smoking-cessation (FDR < 0.05, FC ≥1.7) with a majority of the changes occurring by 8-weeks and a subset changing by 4-weeks. Genes down-regulated by 4- and 8-weeks post-smoking-cessation were involved in xenobiotic metabolism and anti-apoptotic functions respectively. These genes were enriched among genes previously found to be induced in smokers and following short-term in vitro exposure of airway epithelial cells to cigarette smoke (FDR < 0.05). Our findings suggest that the nasal epithelium can serve as a minimally-invasive tool to measure the reversible impact of smoking and broadly, may serve to assess the physiological impact of changes in smoking behavior. |
format | Online Article Text |
id | pubmed-6502805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-65028052019-05-20 Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation Hijazi, Kahkeshan Malyszko, Bozena Steiling, Katrina Xiao, Xiaohui Liu, Gang Alekseyev, Yuriy O. Dumas, Yves-Martine Hertsgaard, Louise Jensen, Joni Hatsukami, Dorothy Brooks, Daniel R. O’Connor, George Beane, Jennifer Lenburg, Marc E. Spira, Avrum Sci Rep Article The physiologic response to tobacco smoke can be measured by gene-expression profiling of the airway epithelium. Temporal resolution of kinetics of gene-expression alterations upon smoking-cessation might delineate distinct biological processes that are activated during recovery from tobacco smoke exposure. Using whole genome gene-expression profiling of individuals initiating a smoking-cessation attempt, we sought to characterize the kinetics of gene-expression alterations in response to short-term smoking-cessation in the nasal epithelium. RNA was extracted from the nasal epithelial of active smokers at baseline and at 4, 8, 16, and 24-weeks after smoking-cessation and put onto Gene ST arrays. Gene-expression levels of 119 genes were associated with smoking-cessation (FDR < 0.05, FC ≥1.7) with a majority of the changes occurring by 8-weeks and a subset changing by 4-weeks. Genes down-regulated by 4- and 8-weeks post-smoking-cessation were involved in xenobiotic metabolism and anti-apoptotic functions respectively. These genes were enriched among genes previously found to be induced in smokers and following short-term in vitro exposure of airway epithelial cells to cigarette smoke (FDR < 0.05). Our findings suggest that the nasal epithelium can serve as a minimally-invasive tool to measure the reversible impact of smoking and broadly, may serve to assess the physiological impact of changes in smoking behavior. Nature Publishing Group UK 2019-05-06 /pmc/articles/PMC6502805/ /pubmed/31061400 http://dx.doi.org/10.1038/s41598-019-43295-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Hijazi, Kahkeshan Malyszko, Bozena Steiling, Katrina Xiao, Xiaohui Liu, Gang Alekseyev, Yuriy O. Dumas, Yves-Martine Hertsgaard, Louise Jensen, Joni Hatsukami, Dorothy Brooks, Daniel R. O’Connor, George Beane, Jennifer Lenburg, Marc E. Spira, Avrum Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation |
title | Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation |
title_full | Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation |
title_fullStr | Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation |
title_full_unstemmed | Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation |
title_short | Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation |
title_sort | tobacco-related alterations in airway gene expression are rapidly reversed within weeks following smoking-cessation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502805/ https://www.ncbi.nlm.nih.gov/pubmed/31061400 http://dx.doi.org/10.1038/s41598-019-43295-3 |
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