Cargando…

Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator

Constrictive vascular remodeling limiting blood flow, as well as compensatory outward remodeling, has been observed in many cardiovascular diseases; however, the underlying mechanisms regulating the remodeling response of the vessels remain unclear. Plasminogen activators (PA) are involved in many o...

Descripción completa

Detalles Bibliográficos
Autores principales: Plekhanova, Olga, Parfyonova, Yelena, Beloglazova, Irina, Berk, Bradford C., Tkachuk, Vsevolod
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502959/
https://www.ncbi.nlm.nih.gov/pubmed/31114508
http://dx.doi.org/10.3389/fphys.2019.00493
_version_ 1783416321417936896
author Plekhanova, Olga
Parfyonova, Yelena
Beloglazova, Irina
Berk, Bradford C.
Tkachuk, Vsevolod
author_facet Plekhanova, Olga
Parfyonova, Yelena
Beloglazova, Irina
Berk, Bradford C.
Tkachuk, Vsevolod
author_sort Plekhanova, Olga
collection PubMed
description Constrictive vascular remodeling limiting blood flow, as well as compensatory outward remodeling, has been observed in many cardiovascular diseases; however, the underlying mechanisms regulating the remodeling response of the vessels remain unclear. Plasminogen activators (PA) are involved in many of the processes of vascular remodeling. We have shown previously that increased levels of tissue-type PA (tPA) contributes to outward vascular remodeling. To elucidate the mechanisms involved in the induction of outward remodeling we characterized changes in the expression profiles of 8799 genes in injured rat carotid arteries 1 and 4 days after recombinant tPA treatment compared to vehicle. Periadventitial tPA significantly increased lumen size and vessel area, encompassed by the external elastic lamina, at both one and 4 days after treatment. Among 41 differentially expressed known genes 1 day after tPA application, five genes were involved in gene transcription, five genes were related to the regulation of vascular tone [for example, thromboxane A2 receptor (D32080) or non-selective-type endothelin receptor (S65355)], and eight genes were identified as participating in vascular innervation [for example, calpain (D14478) or neural cell adhesion molecule L1 (X59149)]. Four days after injury in tPA-treated arteries, four genes, regulating vascular tone, were differentially expressed. Thus, tPA promotes outward arterial remodeling after injury, at least in part, by regulating expression of genes in the vessel wall related to function of the nervous system and vascular tone.
format Online
Article
Text
id pubmed-6502959
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-65029592019-05-21 Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator Plekhanova, Olga Parfyonova, Yelena Beloglazova, Irina Berk, Bradford C. Tkachuk, Vsevolod Front Physiol Physiology Constrictive vascular remodeling limiting blood flow, as well as compensatory outward remodeling, has been observed in many cardiovascular diseases; however, the underlying mechanisms regulating the remodeling response of the vessels remain unclear. Plasminogen activators (PA) are involved in many of the processes of vascular remodeling. We have shown previously that increased levels of tissue-type PA (tPA) contributes to outward vascular remodeling. To elucidate the mechanisms involved in the induction of outward remodeling we characterized changes in the expression profiles of 8799 genes in injured rat carotid arteries 1 and 4 days after recombinant tPA treatment compared to vehicle. Periadventitial tPA significantly increased lumen size and vessel area, encompassed by the external elastic lamina, at both one and 4 days after treatment. Among 41 differentially expressed known genes 1 day after tPA application, five genes were involved in gene transcription, five genes were related to the regulation of vascular tone [for example, thromboxane A2 receptor (D32080) or non-selective-type endothelin receptor (S65355)], and eight genes were identified as participating in vascular innervation [for example, calpain (D14478) or neural cell adhesion molecule L1 (X59149)]. Four days after injury in tPA-treated arteries, four genes, regulating vascular tone, were differentially expressed. Thus, tPA promotes outward arterial remodeling after injury, at least in part, by regulating expression of genes in the vessel wall related to function of the nervous system and vascular tone. Frontiers Media S.A. 2019-04-30 /pmc/articles/PMC6502959/ /pubmed/31114508 http://dx.doi.org/10.3389/fphys.2019.00493 Text en Copyright © 2019 Plekhanova, Parfyonova, Beloglazova, Berk and Tkachuk. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Plekhanova, Olga
Parfyonova, Yelena
Beloglazova, Irina
Berk, Bradford C.
Tkachuk, Vsevolod
Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator
title Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator
title_full Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator
title_fullStr Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator
title_full_unstemmed Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator
title_short Oligonucleotide Microarrays Identified Potential Regulatory Genes Related to Early Outward Arterial Remodeling Induced by Tissue Plasminogen Activator
title_sort oligonucleotide microarrays identified potential regulatory genes related to early outward arterial remodeling induced by tissue plasminogen activator
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502959/
https://www.ncbi.nlm.nih.gov/pubmed/31114508
http://dx.doi.org/10.3389/fphys.2019.00493
work_keys_str_mv AT plekhanovaolga oligonucleotidemicroarraysidentifiedpotentialregulatorygenesrelatedtoearlyoutwardarterialremodelinginducedbytissueplasminogenactivator
AT parfyonovayelena oligonucleotidemicroarraysidentifiedpotentialregulatorygenesrelatedtoearlyoutwardarterialremodelinginducedbytissueplasminogenactivator
AT beloglazovairina oligonucleotidemicroarraysidentifiedpotentialregulatorygenesrelatedtoearlyoutwardarterialremodelinginducedbytissueplasminogenactivator
AT berkbradfordc oligonucleotidemicroarraysidentifiedpotentialregulatorygenesrelatedtoearlyoutwardarterialremodelinginducedbytissueplasminogenactivator
AT tkachukvsevolod oligonucleotidemicroarraysidentifiedpotentialregulatorygenesrelatedtoearlyoutwardarterialremodelinginducedbytissueplasminogenactivator