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Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink

Storage roots are the main sink for photo-assimilate accumulation and reflect cassava yield and productivity. Regulation of sugar partitioning from leaves to storage roots has not been elucidated. Cell wall invertases are involved in the hydrolysis of sugar during phloem unloading of vascular plants...

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Autores principales: Yan, Wei, Wu, Xiaoyun, Li, Yanan, Liu, Guanghua, Cui, Zhanfei, Jiang, Tailing, Ma, Qiuxiang, Luo, Lijuan, Zhang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503109/
https://www.ncbi.nlm.nih.gov/pubmed/31114601
http://dx.doi.org/10.3389/fpls.2019.00541
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author Yan, Wei
Wu, Xiaoyun
Li, Yanan
Liu, Guanghua
Cui, Zhanfei
Jiang, Tailing
Ma, Qiuxiang
Luo, Lijuan
Zhang, Peng
author_facet Yan, Wei
Wu, Xiaoyun
Li, Yanan
Liu, Guanghua
Cui, Zhanfei
Jiang, Tailing
Ma, Qiuxiang
Luo, Lijuan
Zhang, Peng
author_sort Yan, Wei
collection PubMed
description Storage roots are the main sink for photo-assimilate accumulation and reflect cassava yield and productivity. Regulation of sugar partitioning from leaves to storage roots has not been elucidated. Cell wall invertases are involved in the hydrolysis of sugar during phloem unloading of vascular plants to control plant development and sink strength but have rarely been studied in root crops like cassava. MeCWINV3 encodes a typical cell wall invertase in cassava and is mainly expressed in vascular bundles. The gene is highly expressed in leaves, especially mature leaves, in response to diurnal rhythm. When MeCWINV3 was overexpressed in cassava, sugar export from leaves to storage roots was largely inhibited and sucrose hydrolysis in leaves was accelerated, leading to increased transient starch accumulation by blocking starch degradation and reduced overall plant growth. The progress of leaf senescence was promoted in the MeCWINV3 over-expressed cassava plants with increased expression of senescence-related genes. Storage root development was also delayed because of dramatically reduced sugar allocation from leaves. As a result, the transcriptional expression of starch biosynthetic genes such as small subunit ADP-glucose pyrophosphorylase, granule-bound starch synthase I, and starch branching enzyme I was reduced in accordance with insufficient sugar supply in the storage roots of the transgenic plants. These results show that MeCWINV3 regulates sugar allocation from source to sink and maintains sugar balance in cassava, thus affecting yield of cassava storage roots.
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spelling pubmed-65031092019-05-21 Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink Yan, Wei Wu, Xiaoyun Li, Yanan Liu, Guanghua Cui, Zhanfei Jiang, Tailing Ma, Qiuxiang Luo, Lijuan Zhang, Peng Front Plant Sci Plant Science Storage roots are the main sink for photo-assimilate accumulation and reflect cassava yield and productivity. Regulation of sugar partitioning from leaves to storage roots has not been elucidated. Cell wall invertases are involved in the hydrolysis of sugar during phloem unloading of vascular plants to control plant development and sink strength but have rarely been studied in root crops like cassava. MeCWINV3 encodes a typical cell wall invertase in cassava and is mainly expressed in vascular bundles. The gene is highly expressed in leaves, especially mature leaves, in response to diurnal rhythm. When MeCWINV3 was overexpressed in cassava, sugar export from leaves to storage roots was largely inhibited and sucrose hydrolysis in leaves was accelerated, leading to increased transient starch accumulation by blocking starch degradation and reduced overall plant growth. The progress of leaf senescence was promoted in the MeCWINV3 over-expressed cassava plants with increased expression of senescence-related genes. Storage root development was also delayed because of dramatically reduced sugar allocation from leaves. As a result, the transcriptional expression of starch biosynthetic genes such as small subunit ADP-glucose pyrophosphorylase, granule-bound starch synthase I, and starch branching enzyme I was reduced in accordance with insufficient sugar supply in the storage roots of the transgenic plants. These results show that MeCWINV3 regulates sugar allocation from source to sink and maintains sugar balance in cassava, thus affecting yield of cassava storage roots. Frontiers Media S.A. 2019-04-30 /pmc/articles/PMC6503109/ /pubmed/31114601 http://dx.doi.org/10.3389/fpls.2019.00541 Text en Copyright © 2019 Yan, Wu, Li, Liu, Cui, Jiang, Ma, Luo and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yan, Wei
Wu, Xiaoyun
Li, Yanan
Liu, Guanghua
Cui, Zhanfei
Jiang, Tailing
Ma, Qiuxiang
Luo, Lijuan
Zhang, Peng
Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink
title Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink
title_full Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink
title_fullStr Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink
title_full_unstemmed Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink
title_short Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink
title_sort cell wall invertase 3 affects cassava productivity via regulating sugar allocation from source to sink
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503109/
https://www.ncbi.nlm.nih.gov/pubmed/31114601
http://dx.doi.org/10.3389/fpls.2019.00541
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