Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electro...

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Detalles Bibliográficos
Autores principales: Ingegnere, Tiziano, Mariotti, Francesca Romana, Pelosi, Andrea, Quintarelli, Concetta, De Angelis, Biagio, Tumino, Nicola, Besi, Francesca, Cantoni, Claudia, Locatelli, Franco, Vacca, Paola, Moretta, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503170/
https://www.ncbi.nlm.nih.gov/pubmed/31114587
http://dx.doi.org/10.3389/fimmu.2019.00957
Descripción
Sumario:CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

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