Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electro...

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Autores principales: Ingegnere, Tiziano, Mariotti, Francesca Romana, Pelosi, Andrea, Quintarelli, Concetta, De Angelis, Biagio, Tumino, Nicola, Besi, Francesca, Cantoni, Claudia, Locatelli, Franco, Vacca, Paola, Moretta, Lorenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503170/
https://www.ncbi.nlm.nih.gov/pubmed/31114587
http://dx.doi.org/10.3389/fimmu.2019.00957
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author Ingegnere, Tiziano
Mariotti, Francesca Romana
Pelosi, Andrea
Quintarelli, Concetta
De Angelis, Biagio
Tumino, Nicola
Besi, Francesca
Cantoni, Claudia
Locatelli, Franco
Vacca, Paola
Moretta, Lorenzo
author_facet Ingegnere, Tiziano
Mariotti, Francesca Romana
Pelosi, Andrea
Quintarelli, Concetta
De Angelis, Biagio
Tumino, Nicola
Besi, Francesca
Cantoni, Claudia
Locatelli, Franco
Vacca, Paola
Moretta, Lorenzo
author_sort Ingegnere, Tiziano
collection PubMed
description CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.
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spelling pubmed-65031702019-05-21 Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing Ingegnere, Tiziano Mariotti, Francesca Romana Pelosi, Andrea Quintarelli, Concetta De Angelis, Biagio Tumino, Nicola Besi, Francesca Cantoni, Claudia Locatelli, Franco Vacca, Paola Moretta, Lorenzo Front Immunol Immunology CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer. Frontiers Media S.A. 2019-04-30 /pmc/articles/PMC6503170/ /pubmed/31114587 http://dx.doi.org/10.3389/fimmu.2019.00957 Text en Copyright © 2019 Ingegnere, Mariotti, Pelosi, Quintarelli, De Angelis, Tumino, Besi, Cantoni, Locatelli, Vacca and Moretta. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Ingegnere, Tiziano
Mariotti, Francesca Romana
Pelosi, Andrea
Quintarelli, Concetta
De Angelis, Biagio
Tumino, Nicola
Besi, Francesca
Cantoni, Claudia
Locatelli, Franco
Vacca, Paola
Moretta, Lorenzo
Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_full Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_fullStr Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_full_unstemmed Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_short Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing
title_sort human car nk cells: a new non-viral method allowing high efficient transfection and strong tumor cell killing
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503170/
https://www.ncbi.nlm.nih.gov/pubmed/31114587
http://dx.doi.org/10.3389/fimmu.2019.00957
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