Cargando…

Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice

BACKGROUND: Bisphenol A (BPA), a major endocrine disruptor and a xenobiotic compound is used abundantly in the production of polycarbonate plastics and epoxy resins. Human exposure to this compound is primarily via its leaching from the protective internal epoxy resin coatings of containers into the...

Descripción completa

Detalles Bibliográficos
Autores principales: Birla, Hareram, Keswani, Chetan, Rai, Sachchida Nand, Singh, Saumitra Sen, Zahra, Walia, Dilnashin, Hagera, Rathore, Aaina Singh, Singh, Surya Pratap
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503545/
https://www.ncbi.nlm.nih.gov/pubmed/31064381
http://dx.doi.org/10.1186/s12993-019-0160-4
_version_ 1783416430090256384
author Birla, Hareram
Keswani, Chetan
Rai, Sachchida Nand
Singh, Saumitra Sen
Zahra, Walia
Dilnashin, Hagera
Rathore, Aaina Singh
Singh, Surya Pratap
author_facet Birla, Hareram
Keswani, Chetan
Rai, Sachchida Nand
Singh, Saumitra Sen
Zahra, Walia
Dilnashin, Hagera
Rathore, Aaina Singh
Singh, Surya Pratap
author_sort Birla, Hareram
collection PubMed
description BACKGROUND: Bisphenol A (BPA), a major endocrine disruptor and a xenobiotic compound is used abundantly in the production of polycarbonate plastics and epoxy resins. Human exposure to this compound is primarily via its leaching from the protective internal epoxy resin coatings of containers into the food and beverages. In addition, the plastics used in dental prostheses and sealants also contain considerable amount of BPA and have a high risk of human exposure. Since it is a well-known endocrine disruptor and closely mimics the molecular structure of human estrogen thereby impairing learning and memory. Withania somnifera (Ws), commonly known as Ashwagandha is known for its varied therapeutic uses in Ayurvedic system of medicine. The present study was undertaken to demonstrate the impairment induced by BPA on the spatial learning, working memory and its alleviation by Ws in Swiss albino mice. The study was conducted on thirty Swiss albino mice, randomly distributed among three groups: control, BPA and BPA + Ws. The behavioral recovery after treatment with Ws was investigated using the Y-maize and Morris water maize test. Whereas, for the estimation of recovery of NMDA receptor which is related to learning and memory in hippocampus region by western blot and immunohistochemistry. Furthermore, the oxidative stress and antioxidant level was assessed by biochemical tests like MDA, SOD and catalase. RESULTS: The study revealed that administration of Ws alleviated the behavioral deficits induced by BPA. Alongside, Ws treatment reinstated the number of NMDA receptors in hippocampus region and showed anti-oxidative property while ameliorating the endogenous anti-oxidant level in the brain. CONCLUSION: These findings suggest that Ws significantly ameliorates the level of BPA intoxicated oxidative stress thereby potentially treating cognitive dysfunction which acts as the primary symptom in a number of neurodegenerative diseases.
format Online
Article
Text
id pubmed-6503545
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-65035452019-05-10 Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice Birla, Hareram Keswani, Chetan Rai, Sachchida Nand Singh, Saumitra Sen Zahra, Walia Dilnashin, Hagera Rathore, Aaina Singh Singh, Surya Pratap Behav Brain Funct Research BACKGROUND: Bisphenol A (BPA), a major endocrine disruptor and a xenobiotic compound is used abundantly in the production of polycarbonate plastics and epoxy resins. Human exposure to this compound is primarily via its leaching from the protective internal epoxy resin coatings of containers into the food and beverages. In addition, the plastics used in dental prostheses and sealants also contain considerable amount of BPA and have a high risk of human exposure. Since it is a well-known endocrine disruptor and closely mimics the molecular structure of human estrogen thereby impairing learning and memory. Withania somnifera (Ws), commonly known as Ashwagandha is known for its varied therapeutic uses in Ayurvedic system of medicine. The present study was undertaken to demonstrate the impairment induced by BPA on the spatial learning, working memory and its alleviation by Ws in Swiss albino mice. The study was conducted on thirty Swiss albino mice, randomly distributed among three groups: control, BPA and BPA + Ws. The behavioral recovery after treatment with Ws was investigated using the Y-maize and Morris water maize test. Whereas, for the estimation of recovery of NMDA receptor which is related to learning and memory in hippocampus region by western blot and immunohistochemistry. Furthermore, the oxidative stress and antioxidant level was assessed by biochemical tests like MDA, SOD and catalase. RESULTS: The study revealed that administration of Ws alleviated the behavioral deficits induced by BPA. Alongside, Ws treatment reinstated the number of NMDA receptors in hippocampus region and showed anti-oxidative property while ameliorating the endogenous anti-oxidant level in the brain. CONCLUSION: These findings suggest that Ws significantly ameliorates the level of BPA intoxicated oxidative stress thereby potentially treating cognitive dysfunction which acts as the primary symptom in a number of neurodegenerative diseases. BioMed Central 2019-05-07 /pmc/articles/PMC6503545/ /pubmed/31064381 http://dx.doi.org/10.1186/s12993-019-0160-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Birla, Hareram
Keswani, Chetan
Rai, Sachchida Nand
Singh, Saumitra Sen
Zahra, Walia
Dilnashin, Hagera
Rathore, Aaina Singh
Singh, Surya Pratap
Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice
title Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice
title_full Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice
title_fullStr Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice
title_full_unstemmed Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice
title_short Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice
title_sort neuroprotective effects of withania somnifera in bpa induced-cognitive dysfunction and oxidative stress in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503545/
https://www.ncbi.nlm.nih.gov/pubmed/31064381
http://dx.doi.org/10.1186/s12993-019-0160-4
work_keys_str_mv AT birlahareram neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice
AT keswanichetan neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice
AT raisachchidanand neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice
AT singhsaumitrasen neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice
AT zahrawalia neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice
AT dilnashinhagera neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice
AT rathoreaainasingh neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice
AT singhsuryapratap neuroprotectiveeffectsofwithaniasomniferainbpainducedcognitivedysfunctionandoxidativestressinmice