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Experimental 3D coherent diffractive imaging from photon-sparse random projections

The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure–function relationships in systems ranging from energy-storage materials to biological molecules. Extremely bright ultrashort-pulse X-ray sources – X-ray free-elec...

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Autores principales: Giewekemeyer, K., Aquila, A., Loh, N.-T. D., Chushkin, Y., Shanks, K. S., Weiss, J.T., Tate, M. W., Philipp, H. T., Stern, S., Vagovic, P., Mehrjoo, M., Teo, C., Barthelmess, M., Zontone, F., Chang, C., Tiberio, R. C., Sakdinawat, A., Williams, G. J., Gruner, S. M., Mancuso, A. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503918/
https://www.ncbi.nlm.nih.gov/pubmed/31098017
http://dx.doi.org/10.1107/S2052252519002781
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author Giewekemeyer, K.
Aquila, A.
Loh, N.-T. D.
Chushkin, Y.
Shanks, K. S.
Weiss, J.T.
Tate, M. W.
Philipp, H. T.
Stern, S.
Vagovic, P.
Mehrjoo, M.
Teo, C.
Barthelmess, M.
Zontone, F.
Chang, C.
Tiberio, R. C.
Sakdinawat, A.
Williams, G. J.
Gruner, S. M.
Mancuso, A. P.
author_facet Giewekemeyer, K.
Aquila, A.
Loh, N.-T. D.
Chushkin, Y.
Shanks, K. S.
Weiss, J.T.
Tate, M. W.
Philipp, H. T.
Stern, S.
Vagovic, P.
Mehrjoo, M.
Teo, C.
Barthelmess, M.
Zontone, F.
Chang, C.
Tiberio, R. C.
Sakdinawat, A.
Williams, G. J.
Gruner, S. M.
Mancuso, A. P.
author_sort Giewekemeyer, K.
collection PubMed
description The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure–function relationships in systems ranging from energy-storage materials to biological molecules. Extremely bright ultrashort-pulse X-ray sources – X-ray free-electron lasers (XFELs) – provide X-rays that can be used to probe ensembles of nearly identical nanoscale particles. When combined with coherent diffractive imaging, these objects can be imaged; however, as the resolution of the images approaches the atomic scale, the measured data are increasingly difficult to obtain and, during an X-ray pulse, the number of photons incident on the 2D detector is much smaller than the number of pixels. This latter concern, the signal ‘sparsity’, materially impedes the application of the method. An experimental analog using a conventional X-ray source is demonstrated and yields signal levels comparable with those expected from single biomolecules illuminated by focused XFEL pulses. The analog experiment provides an invaluable cross check on the fidelity of the reconstructed data that is not available during XFEL experiments. Using these experimental data, it is established that a sparsity of order 1.3 × 10(−3) photons per pixel per frame can be overcome, lending vital insight to the solution of the atomic resolution XFEL single-particle imaging problem by experimentally demonstrating 3D coherent diffractive imaging from photon-sparse random projections.
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spelling pubmed-65039182019-05-16 Experimental 3D coherent diffractive imaging from photon-sparse random projections Giewekemeyer, K. Aquila, A. Loh, N.-T. D. Chushkin, Y. Shanks, K. S. Weiss, J.T. Tate, M. W. Philipp, H. T. Stern, S. Vagovic, P. Mehrjoo, M. Teo, C. Barthelmess, M. Zontone, F. Chang, C. Tiberio, R. C. Sakdinawat, A. Williams, G. J. Gruner, S. M. Mancuso, A. P. IUCrJ Research Papers The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure–function relationships in systems ranging from energy-storage materials to biological molecules. Extremely bright ultrashort-pulse X-ray sources – X-ray free-electron lasers (XFELs) – provide X-rays that can be used to probe ensembles of nearly identical nanoscale particles. When combined with coherent diffractive imaging, these objects can be imaged; however, as the resolution of the images approaches the atomic scale, the measured data are increasingly difficult to obtain and, during an X-ray pulse, the number of photons incident on the 2D detector is much smaller than the number of pixels. This latter concern, the signal ‘sparsity’, materially impedes the application of the method. An experimental analog using a conventional X-ray source is demonstrated and yields signal levels comparable with those expected from single biomolecules illuminated by focused XFEL pulses. The analog experiment provides an invaluable cross check on the fidelity of the reconstructed data that is not available during XFEL experiments. Using these experimental data, it is established that a sparsity of order 1.3 × 10(−3) photons per pixel per frame can be overcome, lending vital insight to the solution of the atomic resolution XFEL single-particle imaging problem by experimentally demonstrating 3D coherent diffractive imaging from photon-sparse random projections. International Union of Crystallography 2019-03-20 /pmc/articles/PMC6503918/ /pubmed/31098017 http://dx.doi.org/10.1107/S2052252519002781 Text en © K. Giewekemeyer et al. 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Giewekemeyer, K.
Aquila, A.
Loh, N.-T. D.
Chushkin, Y.
Shanks, K. S.
Weiss, J.T.
Tate, M. W.
Philipp, H. T.
Stern, S.
Vagovic, P.
Mehrjoo, M.
Teo, C.
Barthelmess, M.
Zontone, F.
Chang, C.
Tiberio, R. C.
Sakdinawat, A.
Williams, G. J.
Gruner, S. M.
Mancuso, A. P.
Experimental 3D coherent diffractive imaging from photon-sparse random projections
title Experimental 3D coherent diffractive imaging from photon-sparse random projections
title_full Experimental 3D coherent diffractive imaging from photon-sparse random projections
title_fullStr Experimental 3D coherent diffractive imaging from photon-sparse random projections
title_full_unstemmed Experimental 3D coherent diffractive imaging from photon-sparse random projections
title_short Experimental 3D coherent diffractive imaging from photon-sparse random projections
title_sort experimental 3d coherent diffractive imaging from photon-sparse random projections
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6503918/
https://www.ncbi.nlm.nih.gov/pubmed/31098017
http://dx.doi.org/10.1107/S2052252519002781
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