Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation

Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified...

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Autores principales: García-Silva, Susana, Benito-Martín, Alberto, Sánchez-Redondo, Sara, Hernández-Barranco, Alberto, Ximénez-Embún, Pilar, Nogués, Laura, Mazariegos, Marina S., Brinkmann, Kay, Amor López, Ana, Meyer, Lisa, Rodríguez, Carlos, García-Martín, Carmen, Boskovic, Jasminka, Letón, Rocío, Montero, Cristina, Robledo, Mercedes, Santambrogio, Laura, Sue Brady, Mary, Szumera-Ciećkiewicz, Anna, Kalinowska, Iwona, Skog, Johan, Noerholm, Mikkel, Muñoz, Javier, Ortiz-Romero, Pablo L., Ruano, Yolanda, Rodríguez-Peralto, José L., Rutkowski, Piotr, Peinado, Héctor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504207/
https://www.ncbi.nlm.nih.gov/pubmed/30975894
http://dx.doi.org/10.1084/jem.20181522
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author García-Silva, Susana
Benito-Martín, Alberto
Sánchez-Redondo, Sara
Hernández-Barranco, Alberto
Ximénez-Embún, Pilar
Nogués, Laura
Mazariegos, Marina S.
Brinkmann, Kay
Amor López, Ana
Meyer, Lisa
Rodríguez, Carlos
García-Martín, Carmen
Boskovic, Jasminka
Letón, Rocío
Montero, Cristina
Robledo, Mercedes
Santambrogio, Laura
Sue Brady, Mary
Szumera-Ciećkiewicz, Anna
Kalinowska, Iwona
Skog, Johan
Noerholm, Mikkel
Muñoz, Javier
Ortiz-Romero, Pablo L.
Ruano, Yolanda
Rodríguez-Peralto, José L.
Rutkowski, Piotr
Peinado, Héctor
author_facet García-Silva, Susana
Benito-Martín, Alberto
Sánchez-Redondo, Sara
Hernández-Barranco, Alberto
Ximénez-Embún, Pilar
Nogués, Laura
Mazariegos, Marina S.
Brinkmann, Kay
Amor López, Ana
Meyer, Lisa
Rodríguez, Carlos
García-Martín, Carmen
Boskovic, Jasminka
Letón, Rocío
Montero, Cristina
Robledo, Mercedes
Santambrogio, Laura
Sue Brady, Mary
Szumera-Ciećkiewicz, Anna
Kalinowska, Iwona
Skog, Johan
Noerholm, Mikkel
Muñoz, Javier
Ortiz-Romero, Pablo L.
Ruano, Yolanda
Rodríguez-Peralto, José L.
Rutkowski, Piotr
Peinado, Héctor
author_sort García-Silva, Susana
collection PubMed
description Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAF(V600E) mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse.
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spelling pubmed-65042072019-11-06 Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation García-Silva, Susana Benito-Martín, Alberto Sánchez-Redondo, Sara Hernández-Barranco, Alberto Ximénez-Embún, Pilar Nogués, Laura Mazariegos, Marina S. Brinkmann, Kay Amor López, Ana Meyer, Lisa Rodríguez, Carlos García-Martín, Carmen Boskovic, Jasminka Letón, Rocío Montero, Cristina Robledo, Mercedes Santambrogio, Laura Sue Brady, Mary Szumera-Ciećkiewicz, Anna Kalinowska, Iwona Skog, Johan Noerholm, Mikkel Muñoz, Javier Ortiz-Romero, Pablo L. Ruano, Yolanda Rodríguez-Peralto, José L. Rutkowski, Piotr Peinado, Héctor J Exp Med Research Articles Liquid biopsies from cancer patients have the potential to improve diagnosis and prognosis. The assessment of surrogate markers of tumor progression in circulating extracellular vesicles could be a powerful non-invasive approach in this setting. We have characterized extracellular vesicles purified from the lymphatic drainage also known as exudative seroma (ES) of stage III melanoma patients obtained after lymphadenectomy. Proteomic analysis showed that seroma-derived exosomes are enriched in proteins resembling melanoma progression. In addition, we found that the BRAF(V600E) mutation can be detected in ES-derived extracellular vesicles and its detection correlated with patients at risk of relapse. Rockefeller University Press 2019-05-06 2019-04-11 /pmc/articles/PMC6504207/ /pubmed/30975894 http://dx.doi.org/10.1084/jem.20181522 Text en © 2019 García-Silva et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
García-Silva, Susana
Benito-Martín, Alberto
Sánchez-Redondo, Sara
Hernández-Barranco, Alberto
Ximénez-Embún, Pilar
Nogués, Laura
Mazariegos, Marina S.
Brinkmann, Kay
Amor López, Ana
Meyer, Lisa
Rodríguez, Carlos
García-Martín, Carmen
Boskovic, Jasminka
Letón, Rocío
Montero, Cristina
Robledo, Mercedes
Santambrogio, Laura
Sue Brady, Mary
Szumera-Ciećkiewicz, Anna
Kalinowska, Iwona
Skog, Johan
Noerholm, Mikkel
Muñoz, Javier
Ortiz-Romero, Pablo L.
Ruano, Yolanda
Rodríguez-Peralto, José L.
Rutkowski, Piotr
Peinado, Héctor
Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation
title Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation
title_full Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation
title_fullStr Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation
title_full_unstemmed Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation
title_short Use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and BRAF(V600E) mutation
title_sort use of extracellular vesicles from lymphatic drainage as surrogate markers of melanoma progression and braf(v600e) mutation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504207/
https://www.ncbi.nlm.nih.gov/pubmed/30975894
http://dx.doi.org/10.1084/jem.20181522
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