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Aire-expressing ILC3-like cells in the lymph node display potent APC features
The autoimmune regulator (Aire) serves an essential function for T cell tolerance by promoting the “promiscuous” expression of tissue antigens in thymic epithelial cells. Aire is also detected in rare cells in peripheral lymphoid organs, but the identity of these cells is poorly understood. Here, we...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504225/ https://www.ncbi.nlm.nih.gov/pubmed/30918005 http://dx.doi.org/10.1084/jem.20181430 |
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author | Yamano, Tomoyoshi Dobeš, Jan Vobořil, Matouš Steinert, Madlen Brabec, Tomáš Ziętara, Natalia Dobešová, Martina Ohnmacht, Caspar Laan, Martti Peterson, Part Benes, Vladimir Sedláček, Radislav Hanayama, Rikinari Kolář, Michal Klein, Ludger Filipp, Dominik |
author_facet | Yamano, Tomoyoshi Dobeš, Jan Vobořil, Matouš Steinert, Madlen Brabec, Tomáš Ziętara, Natalia Dobešová, Martina Ohnmacht, Caspar Laan, Martti Peterson, Part Benes, Vladimir Sedláček, Radislav Hanayama, Rikinari Kolář, Michal Klein, Ludger Filipp, Dominik |
author_sort | Yamano, Tomoyoshi |
collection | PubMed |
description | The autoimmune regulator (Aire) serves an essential function for T cell tolerance by promoting the “promiscuous” expression of tissue antigens in thymic epithelial cells. Aire is also detected in rare cells in peripheral lymphoid organs, but the identity of these cells is poorly understood. Here, we report that Aire protein–expressing cells in lymph nodes exhibit typical group 3 innate lymphoid cell (ILC3) characteristics such as lymphoid morphology, absence of “classical” hematopoietic lineage markers, and dependence on RORγt. Aire(+) cells are more frequent among lineage-negative RORγt(+) cells of peripheral lymph nodes as compared with mucosa-draining lymph nodes, display a unique Aire-dependent transcriptional signature, express high surface levels of MHCII and costimulatory molecules, and efficiently present an endogenously expressed model antigen to CD4(+) T cells. These findings define a novel type of ILC3-like cells with potent APC features, suggesting that these cells serve a function in the control of T cell responses. |
format | Online Article Text |
id | pubmed-6504225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-65042252019-11-06 Aire-expressing ILC3-like cells in the lymph node display potent APC features Yamano, Tomoyoshi Dobeš, Jan Vobořil, Matouš Steinert, Madlen Brabec, Tomáš Ziętara, Natalia Dobešová, Martina Ohnmacht, Caspar Laan, Martti Peterson, Part Benes, Vladimir Sedláček, Radislav Hanayama, Rikinari Kolář, Michal Klein, Ludger Filipp, Dominik J Exp Med Research Articles The autoimmune regulator (Aire) serves an essential function for T cell tolerance by promoting the “promiscuous” expression of tissue antigens in thymic epithelial cells. Aire is also detected in rare cells in peripheral lymphoid organs, but the identity of these cells is poorly understood. Here, we report that Aire protein–expressing cells in lymph nodes exhibit typical group 3 innate lymphoid cell (ILC3) characteristics such as lymphoid morphology, absence of “classical” hematopoietic lineage markers, and dependence on RORγt. Aire(+) cells are more frequent among lineage-negative RORγt(+) cells of peripheral lymph nodes as compared with mucosa-draining lymph nodes, display a unique Aire-dependent transcriptional signature, express high surface levels of MHCII and costimulatory molecules, and efficiently present an endogenously expressed model antigen to CD4(+) T cells. These findings define a novel type of ILC3-like cells with potent APC features, suggesting that these cells serve a function in the control of T cell responses. Rockefeller University Press 2019-05-06 2019-03-27 /pmc/articles/PMC6504225/ /pubmed/30918005 http://dx.doi.org/10.1084/jem.20181430 Text en © 2019 Yamano et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Yamano, Tomoyoshi Dobeš, Jan Vobořil, Matouš Steinert, Madlen Brabec, Tomáš Ziętara, Natalia Dobešová, Martina Ohnmacht, Caspar Laan, Martti Peterson, Part Benes, Vladimir Sedláček, Radislav Hanayama, Rikinari Kolář, Michal Klein, Ludger Filipp, Dominik Aire-expressing ILC3-like cells in the lymph node display potent APC features |
title | Aire-expressing ILC3-like cells in the lymph node display potent APC features |
title_full | Aire-expressing ILC3-like cells in the lymph node display potent APC features |
title_fullStr | Aire-expressing ILC3-like cells in the lymph node display potent APC features |
title_full_unstemmed | Aire-expressing ILC3-like cells in the lymph node display potent APC features |
title_short | Aire-expressing ILC3-like cells in the lymph node display potent APC features |
title_sort | aire-expressing ilc3-like cells in the lymph node display potent apc features |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504225/ https://www.ncbi.nlm.nih.gov/pubmed/30918005 http://dx.doi.org/10.1084/jem.20181430 |
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