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Development of a new monoclonal antibody specific to mouse Vγ6 chain
There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504751/ https://www.ncbi.nlm.nih.gov/pubmed/31064767 http://dx.doi.org/10.26508/lsa.201900363 |
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author | Hatano, Shinya Tun, Xin Noguchi, Naoto Yue, Dan Yamada, Hisakata Sun, Xun Matsumoto, Mitsuru Yoshikai, Yasunobu |
author_facet | Hatano, Shinya Tun, Xin Noguchi, Naoto Yue, Dan Yamada, Hisakata Sun, Xun Matsumoto, Mitsuru Yoshikai, Yasunobu |
author_sort | Hatano, Shinya |
collection | PubMed |
description | There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene–transfected T-cell line lacking TCR as well as of Vγ1(−) Vγ4(−) Vγ5(−) Vγ7(−) γδ T cells and the CD3(high) TCRδ(int) γδ T cells in various organs. The location of Vγ6(+) γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice. |
format | Online Article Text |
id | pubmed-6504751 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-65047512019-05-21 Development of a new monoclonal antibody specific to mouse Vγ6 chain Hatano, Shinya Tun, Xin Noguchi, Naoto Yue, Dan Yamada, Hisakata Sun, Xun Matsumoto, Mitsuru Yoshikai, Yasunobu Life Sci Alliance Resources There are seven Vγ gene segments in the TCR γ chain loci of mice. We developed monoclonal antibodies (mAbs) specific to the Vγ6 chain (Heilig & Tonegawa nomenclature). By immunizing Vγ4/6 KO mice with complementarity-determining region peptides in Vγ6 chains, we generated three hybridomas. These hybridomas produced mAbs capable of cell surface staining of Vγ6/Vδ1 gene–transfected T-cell line lacking TCR as well as of Vγ1(−) Vγ4(−) Vγ5(−) Vγ7(−) γδ T cells and the CD3(high) TCRδ(int) γδ T cells in various organs. The location of Vγ6(+) γδ T cells, which peaked in the newborn thymus, was associated with mTEC. In vivo administration of clone 1C10-1F7 mAb impaired protection against Klebsiella pneumoniae infection but ameliorated psoriasis-like dermatitis induced by imiquimod treatment. These new mAbs are useful to elucidate the development, location, and functions of Vγ6 γδ T cells in mice. Life Science Alliance LLC 2019-05-07 /pmc/articles/PMC6504751/ /pubmed/31064767 http://dx.doi.org/10.26508/lsa.201900363 Text en © 2019 Hatano et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Resources Hatano, Shinya Tun, Xin Noguchi, Naoto Yue, Dan Yamada, Hisakata Sun, Xun Matsumoto, Mitsuru Yoshikai, Yasunobu Development of a new monoclonal antibody specific to mouse Vγ6 chain |
title | Development of a new monoclonal antibody specific to mouse Vγ6 chain |
title_full | Development of a new monoclonal antibody specific to mouse Vγ6 chain |
title_fullStr | Development of a new monoclonal antibody specific to mouse Vγ6 chain |
title_full_unstemmed | Development of a new monoclonal antibody specific to mouse Vγ6 chain |
title_short | Development of a new monoclonal antibody specific to mouse Vγ6 chain |
title_sort | development of a new monoclonal antibody specific to mouse vγ6 chain |
topic | Resources |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6504751/ https://www.ncbi.nlm.nih.gov/pubmed/31064767 http://dx.doi.org/10.26508/lsa.201900363 |
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