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Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis

A rapid and sensitive method to determine the characteristics of carcinogens is needed. In this study, we used a microarray-based genomics approach, with a short-term in vivo model, in combination with insights from statistical and mechanistic analyses to determine the characteristics of carcinogens...

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Autores principales: Lee, Seul Ji, Yum, Young Na, Kim, Sang Cheol, Kim, Yuneung, Lim, Johan, Lee, Won Jun, Koo, Kyung Hye, Kim, Joo Hwan, Kim, Jee Eun, Lee, Woo Sun, Sohn, Soojung, Park, Sue Nie, Park, Jeong Hill, Lee, Jeongmi, Kwon, Sung Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505678/
https://www.ncbi.nlm.nih.gov/pubmed/24089152
http://dx.doi.org/10.1038/srep02783
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author Lee, Seul Ji
Yum, Young Na
Kim, Sang Cheol
Kim, Yuneung
Lim, Johan
Lee, Won Jun
Koo, Kyung Hye
Kim, Joo Hwan
Kim, Jee Eun
Lee, Woo Sun
Sohn, Soojung
Park, Sue Nie
Park, Jeong Hill
Lee, Jeongmi
Kwon, Sung Won
author_facet Lee, Seul Ji
Yum, Young Na
Kim, Sang Cheol
Kim, Yuneung
Lim, Johan
Lee, Won Jun
Koo, Kyung Hye
Kim, Joo Hwan
Kim, Jee Eun
Lee, Woo Sun
Sohn, Soojung
Park, Sue Nie
Park, Jeong Hill
Lee, Jeongmi
Kwon, Sung Won
author_sort Lee, Seul Ji
collection PubMed
description A rapid and sensitive method to determine the characteristics of carcinogens is needed. In this study, we used a microarray-based genomics approach, with a short-term in vivo model, in combination with insights from statistical and mechanistic analyses to determine the characteristics of carcinogens. Carcinogens were evaluated based on the different mechanisms involved in the responses to genotoxic carcinogens and non-genotoxic carcinogens. Gene profiling was performed at two time points after treatment with six training and four test carcinogens. We mapped the DEG (differentially expressed gene)-related pathways to analyze cellular processes, and we discovered significant mechanisms that involve critical cellular components. Classification results were further supported by Comet and Micronucleus assays. Mechanistic studies based on gene expression profiling enhanced our understanding of the characteristics of different carcinogens. Moreover, the efficiency of this study was demonstrated by the short-term nature of the animal experiments that were conducted.
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spelling pubmed-65056782019-05-21 Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis Lee, Seul Ji Yum, Young Na Kim, Sang Cheol Kim, Yuneung Lim, Johan Lee, Won Jun Koo, Kyung Hye Kim, Joo Hwan Kim, Jee Eun Lee, Woo Sun Sohn, Soojung Park, Sue Nie Park, Jeong Hill Lee, Jeongmi Kwon, Sung Won Sci Rep Article A rapid and sensitive method to determine the characteristics of carcinogens is needed. In this study, we used a microarray-based genomics approach, with a short-term in vivo model, in combination with insights from statistical and mechanistic analyses to determine the characteristics of carcinogens. Carcinogens were evaluated based on the different mechanisms involved in the responses to genotoxic carcinogens and non-genotoxic carcinogens. Gene profiling was performed at two time points after treatment with six training and four test carcinogens. We mapped the DEG (differentially expressed gene)-related pathways to analyze cellular processes, and we discovered significant mechanisms that involve critical cellular components. Classification results were further supported by Comet and Micronucleus assays. Mechanistic studies based on gene expression profiling enhanced our understanding of the characteristics of different carcinogens. Moreover, the efficiency of this study was demonstrated by the short-term nature of the animal experiments that were conducted. Nature Publishing Group 2013-10-03 /pmc/articles/PMC6505678/ /pubmed/24089152 http://dx.doi.org/10.1038/srep02783 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Lee, Seul Ji
Yum, Young Na
Kim, Sang Cheol
Kim, Yuneung
Lim, Johan
Lee, Won Jun
Koo, Kyung Hye
Kim, Joo Hwan
Kim, Jee Eun
Lee, Woo Sun
Sohn, Soojung
Park, Sue Nie
Park, Jeong Hill
Lee, Jeongmi
Kwon, Sung Won
Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis
title Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis
title_full Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis
title_fullStr Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis
title_full_unstemmed Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis
title_short Distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis
title_sort distinguishing between genotoxic and non-genotoxic hepatocarcinogens by gene expression profiling and bioinformatic pathway analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505678/
https://www.ncbi.nlm.nih.gov/pubmed/24089152
http://dx.doi.org/10.1038/srep02783
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