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Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate
Chronic wounds are projected to reach epidemic proportions worldwide because of the aging population and the increasing incidence of diabetes. Despite extensive research, infection remains one of the leading sources of complications in chronic wounds, resulting in improper healing, biofilm formation...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506339/ https://www.ncbi.nlm.nih.gov/pubmed/31123722 http://dx.doi.org/10.1063/1.5088801 |
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author | Cereceres, Stacy Lan, Ziyang Bryan, Laura Whitely, Michael Wilems, Thomas Greer, Hunter Alexander, Ellen Ruth Taylor, Robert J. Bernstein, Lawrence Cohen, Noah Whitfield-Cargile, Canaan Cosgriff-Hernandez, Elizabeth |
author_facet | Cereceres, Stacy Lan, Ziyang Bryan, Laura Whitely, Michael Wilems, Thomas Greer, Hunter Alexander, Ellen Ruth Taylor, Robert J. Bernstein, Lawrence Cohen, Noah Whitfield-Cargile, Canaan Cosgriff-Hernandez, Elizabeth |
author_sort | Cereceres, Stacy |
collection | PubMed |
description | Chronic wounds are projected to reach epidemic proportions worldwide because of the aging population and the increasing incidence of diabetes. Despite extensive research, infection remains one of the leading sources of complications in chronic wounds, resulting in improper healing, biofilm formation, and lower extremity amputation. To address the limitations of standard treatments, we have developed a hydrogel wound dressing with self-tuning moisture control that incorporates a novel antimicrobial agent to eliminate and prevent infection. 3D-printing of a hydrogel dressing with dual porosity resulted in a new dressing with greater flexibility, increased water uptake, and more rapid swelling than bulk hydrogel dressings. Additionally, gallium maltolate (GaM) was incorporated into the dressing to investigate the efficacy of this antimicrobial agent. Loading profiles, release kinetics, and the bactericidal activity against Staphylococcus aureus (including methicillin-resistant Staphylococcus aureus) of GaM were investigated in vitro to identify target profiles that supported infection control. Finally, GaM-loaded hydrogel dressings were evaluated in vivo, utilizing a murine splinted-wound model that was inoculated with S. aureus. In comparison to an untreated control, GaM dressings markedly reduced the wound bacterial load without compromising wound closure rates. Overall, this work demonstrates the utility of a 3D-printed hydrogel dressing as an antimicrobial dressing to control infection in chronic wounds. |
format | Online Article Text |
id | pubmed-6506339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-65063392019-05-23 Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate Cereceres, Stacy Lan, Ziyang Bryan, Laura Whitely, Michael Wilems, Thomas Greer, Hunter Alexander, Ellen Ruth Taylor, Robert J. Bernstein, Lawrence Cohen, Noah Whitfield-Cargile, Canaan Cosgriff-Hernandez, Elizabeth APL Bioeng Articles Chronic wounds are projected to reach epidemic proportions worldwide because of the aging population and the increasing incidence of diabetes. Despite extensive research, infection remains one of the leading sources of complications in chronic wounds, resulting in improper healing, biofilm formation, and lower extremity amputation. To address the limitations of standard treatments, we have developed a hydrogel wound dressing with self-tuning moisture control that incorporates a novel antimicrobial agent to eliminate and prevent infection. 3D-printing of a hydrogel dressing with dual porosity resulted in a new dressing with greater flexibility, increased water uptake, and more rapid swelling than bulk hydrogel dressings. Additionally, gallium maltolate (GaM) was incorporated into the dressing to investigate the efficacy of this antimicrobial agent. Loading profiles, release kinetics, and the bactericidal activity against Staphylococcus aureus (including methicillin-resistant Staphylococcus aureus) of GaM were investigated in vitro to identify target profiles that supported infection control. Finally, GaM-loaded hydrogel dressings were evaluated in vivo, utilizing a murine splinted-wound model that was inoculated with S. aureus. In comparison to an untreated control, GaM dressings markedly reduced the wound bacterial load without compromising wound closure rates. Overall, this work demonstrates the utility of a 3D-printed hydrogel dressing as an antimicrobial dressing to control infection in chronic wounds. AIP Publishing LLC 2019-04-29 /pmc/articles/PMC6506339/ /pubmed/31123722 http://dx.doi.org/10.1063/1.5088801 Text en © 2019 Author(s). 2473-2877/2019/3(2)/026102/12 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Articles Cereceres, Stacy Lan, Ziyang Bryan, Laura Whitely, Michael Wilems, Thomas Greer, Hunter Alexander, Ellen Ruth Taylor, Robert J. Bernstein, Lawrence Cohen, Noah Whitfield-Cargile, Canaan Cosgriff-Hernandez, Elizabeth Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate |
title | Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate |
title_full | Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate |
title_fullStr | Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate |
title_full_unstemmed | Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate |
title_short | Bactericidal activity of 3D-printed hydrogel dressing loaded with gallium maltolate |
title_sort | bactericidal activity of 3d-printed hydrogel dressing loaded with gallium maltolate |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506339/ https://www.ncbi.nlm.nih.gov/pubmed/31123722 http://dx.doi.org/10.1063/1.5088801 |
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