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Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels

Cartilage loss is a leading cause of disability among adults and effective therapy remains elusive. Neonatal chondrocytes (NChons) are an attractive allogeneic cell source for cartilage repair, but their clinical translation has been hindered by scarce donor availability. Here we examine the potenti...

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Detalles Bibliográficos
Autores principales: Lai, Janice H., Kajiyama, Glen, Smith, Robert Lane, Maloney, William, Yang, Fan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506564/
https://www.ncbi.nlm.nih.gov/pubmed/24352100
http://dx.doi.org/10.1038/srep03553
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author Lai, Janice H.
Kajiyama, Glen
Smith, Robert Lane
Maloney, William
Yang, Fan
author_facet Lai, Janice H.
Kajiyama, Glen
Smith, Robert Lane
Maloney, William
Yang, Fan
author_sort Lai, Janice H.
collection PubMed
description Cartilage loss is a leading cause of disability among adults and effective therapy remains elusive. Neonatal chondrocytes (NChons) are an attractive allogeneic cell source for cartilage repair, but their clinical translation has been hindered by scarce donor availability. Here we examine the potential for catalyzing cartilage tissue formation using a minimal number of NChons by co-culturing them with adipose-derived stem cells (ADSCs) in 3D hydrogels. Using three different co-culture models, we demonstrated that the effects of co-culture on cartilage tissue formation are dependent on the intercellular distance and cell distribution in 3D. Unexpectedly, increasing ADSC ratio in mixed co-culture led to increased synergy between NChons and ADSCs, and resulted in the formation of large neocartilage nodules. This work raises the potential of utilizing stem cells to catalyze tissue formation by neonatal chondrocytes via paracrine signaling, and highlights the importance of controlling cell distribution in 3D matrices to achieve optimal synergy.
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spelling pubmed-65065642019-05-21 Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels Lai, Janice H. Kajiyama, Glen Smith, Robert Lane Maloney, William Yang, Fan Sci Rep Article Cartilage loss is a leading cause of disability among adults and effective therapy remains elusive. Neonatal chondrocytes (NChons) are an attractive allogeneic cell source for cartilage repair, but their clinical translation has been hindered by scarce donor availability. Here we examine the potential for catalyzing cartilage tissue formation using a minimal number of NChons by co-culturing them with adipose-derived stem cells (ADSCs) in 3D hydrogels. Using three different co-culture models, we demonstrated that the effects of co-culture on cartilage tissue formation are dependent on the intercellular distance and cell distribution in 3D. Unexpectedly, increasing ADSC ratio in mixed co-culture led to increased synergy between NChons and ADSCs, and resulted in the formation of large neocartilage nodules. This work raises the potential of utilizing stem cells to catalyze tissue formation by neonatal chondrocytes via paracrine signaling, and highlights the importance of controlling cell distribution in 3D matrices to achieve optimal synergy. Nature Publishing Group 2013-12-19 /pmc/articles/PMC6506564/ /pubmed/24352100 http://dx.doi.org/10.1038/srep03553 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Lai, Janice H.
Kajiyama, Glen
Smith, Robert Lane
Maloney, William
Yang, Fan
Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels
title Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels
title_full Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels
title_fullStr Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels
title_full_unstemmed Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels
title_short Stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3D biomimetic hydrogels
title_sort stem cells catalyze cartilage formation by neonatal articular chondrocytes in 3d biomimetic hydrogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506564/
https://www.ncbi.nlm.nih.gov/pubmed/24352100
http://dx.doi.org/10.1038/srep03553
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