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Stages identifying and transcriptome profiling of the floral transition in Juglans regia

Using paraffin sections, the stages of walnut female flower bud differentiation were divided into the predifferentiation period (F_1), initial differentiation period (F_2) and flower primordium differentiation period (F_3). Leaf buds collected at the same stage as F_2 were designated JRL. Transcript...

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Autores principales: Quan, Shaowen, Niu, Jianxin, Zhou, Li, Xu, Hang, Ma, Li, Qin, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506622/
https://www.ncbi.nlm.nih.gov/pubmed/31068628
http://dx.doi.org/10.1038/s41598-019-43582-z
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author Quan, Shaowen
Niu, Jianxin
Zhou, Li
Xu, Hang
Ma, Li
Qin, Yang
author_facet Quan, Shaowen
Niu, Jianxin
Zhou, Li
Xu, Hang
Ma, Li
Qin, Yang
author_sort Quan, Shaowen
collection PubMed
description Using paraffin sections, the stages of walnut female flower bud differentiation were divided into the predifferentiation period (F_1), initial differentiation period (F_2) and flower primordium differentiation period (F_3). Leaf buds collected at the same stage as F_2 were designated JRL. Transcriptomic profiling was performed, and a total of 132,154 unigenes were obtained with lengths ranging from 201 bp to 16,831 bp. The analysis of differentially expressed genes (DEGs) showed that there were 597, 784 and 532 DEGs in the three combinations F_1vsF_2, F_1vsF_3, and F_2vsF_3, respectively. The comparison F_2vsJRL showed that 374 DEGs were differentially expressed between female buds and leaf buds. Thirty-one DEGs related to flowering time were further used to construct coexpression networks, and CRY2 and NF-YA were identified as core DEGs in flowering time regulation. Eighteen DEGs related to flowering time were subjected to real-time quantitative analysis. Our work provides a foundation for further research on the walnut floral transition and provides new resources for future research on walnut biology and biotechnology.
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spelling pubmed-65066222019-05-21 Stages identifying and transcriptome profiling of the floral transition in Juglans regia Quan, Shaowen Niu, Jianxin Zhou, Li Xu, Hang Ma, Li Qin, Yang Sci Rep Article Using paraffin sections, the stages of walnut female flower bud differentiation were divided into the predifferentiation period (F_1), initial differentiation period (F_2) and flower primordium differentiation period (F_3). Leaf buds collected at the same stage as F_2 were designated JRL. Transcriptomic profiling was performed, and a total of 132,154 unigenes were obtained with lengths ranging from 201 bp to 16,831 bp. The analysis of differentially expressed genes (DEGs) showed that there were 597, 784 and 532 DEGs in the three combinations F_1vsF_2, F_1vsF_3, and F_2vsF_3, respectively. The comparison F_2vsJRL showed that 374 DEGs were differentially expressed between female buds and leaf buds. Thirty-one DEGs related to flowering time were further used to construct coexpression networks, and CRY2 and NF-YA were identified as core DEGs in flowering time regulation. Eighteen DEGs related to flowering time were subjected to real-time quantitative analysis. Our work provides a foundation for further research on the walnut floral transition and provides new resources for future research on walnut biology and biotechnology. Nature Publishing Group UK 2019-05-08 /pmc/articles/PMC6506622/ /pubmed/31068628 http://dx.doi.org/10.1038/s41598-019-43582-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Quan, Shaowen
Niu, Jianxin
Zhou, Li
Xu, Hang
Ma, Li
Qin, Yang
Stages identifying and transcriptome profiling of the floral transition in Juglans regia
title Stages identifying and transcriptome profiling of the floral transition in Juglans regia
title_full Stages identifying and transcriptome profiling of the floral transition in Juglans regia
title_fullStr Stages identifying and transcriptome profiling of the floral transition in Juglans regia
title_full_unstemmed Stages identifying and transcriptome profiling of the floral transition in Juglans regia
title_short Stages identifying and transcriptome profiling of the floral transition in Juglans regia
title_sort stages identifying and transcriptome profiling of the floral transition in juglans regia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506622/
https://www.ncbi.nlm.nih.gov/pubmed/31068628
http://dx.doi.org/10.1038/s41598-019-43582-z
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