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β-Catenin expression is associated with cell invasiveness in pancreatic cancer
BACKGROUND/AIMS: This study was tried to determine the role of β-catenin in invasion in pancreatic cancer. METHODS: We analyzed cancer invasiveness according to β-catenin expression in pancreatic cancer cell line. We also investigated the change in cancer invasiveness when β-catenin expression was c...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Association of Internal Medicine
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506732/ https://www.ncbi.nlm.nih.gov/pubmed/30360026 http://dx.doi.org/10.3904/kjim.2017.155 |
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author | Nan, Jin Niang Kim, Ok Ran Lee, Myung Ah |
author_facet | Nan, Jin Niang Kim, Ok Ran Lee, Myung Ah |
author_sort | Nan, Jin Niang |
collection | PubMed |
description | BACKGROUND/AIMS: This study was tried to determine the role of β-catenin in invasion in pancreatic cancer. METHODS: We analyzed cancer invasiveness according to β-catenin expression in pancreatic cancer cell line. We also investigated the change in cancer invasiveness when β-catenin expression was changed. To enhance β-catenin activity, we treated low β-catenin cancer cell line, PANC1, with Wnt-3a conditioned media and transected β-catenin. We also treated high β-catenin expressing cell line, BxPC3, with XAV939, β-catenin inhibitor and siRNA for β-catenin to inhibit β-catenin expression. RESULTS: The high β-catenin expressing cancer cell line, BxPC3 showed higher invasiveness, and low β-catenin expressing cell lines, PANC1and MIA-PaCa-2, were less invasive. By adding the Wnt-3a conditioned media or performing transfection with β-catenin in PANC1, cell invasiveness was increased (p < 0.05 and p < 0.01, respectively). On inhibition of β-catenin by XAV939 and siRNA in BxPC3 cell line, invasiveness was significantly decreased (p < 0.01). It was not correlated with the expression of cluster of differentiation 44 (CD44) or CD44 variant 6 (CD44v6), the invasion related protein. On analysis of association with metastasis in human tissue, Wnt-3a expression was statistically correlated with the development of metastasis (p = 0.029). CONCLUSIONS: Based on our data, β-catenin may be involved in cancer invasion in pancreatic cancer, and it is not associated with CD44, the invasion related protein. |
format | Online Article Text |
id | pubmed-6506732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-65067322019-05-20 β-Catenin expression is associated with cell invasiveness in pancreatic cancer Nan, Jin Niang Kim, Ok Ran Lee, Myung Ah Korean J Intern Med Original Article BACKGROUND/AIMS: This study was tried to determine the role of β-catenin in invasion in pancreatic cancer. METHODS: We analyzed cancer invasiveness according to β-catenin expression in pancreatic cancer cell line. We also investigated the change in cancer invasiveness when β-catenin expression was changed. To enhance β-catenin activity, we treated low β-catenin cancer cell line, PANC1, with Wnt-3a conditioned media and transected β-catenin. We also treated high β-catenin expressing cell line, BxPC3, with XAV939, β-catenin inhibitor and siRNA for β-catenin to inhibit β-catenin expression. RESULTS: The high β-catenin expressing cancer cell line, BxPC3 showed higher invasiveness, and low β-catenin expressing cell lines, PANC1and MIA-PaCa-2, were less invasive. By adding the Wnt-3a conditioned media or performing transfection with β-catenin in PANC1, cell invasiveness was increased (p < 0.05 and p < 0.01, respectively). On inhibition of β-catenin by XAV939 and siRNA in BxPC3 cell line, invasiveness was significantly decreased (p < 0.01). It was not correlated with the expression of cluster of differentiation 44 (CD44) or CD44 variant 6 (CD44v6), the invasion related protein. On analysis of association with metastasis in human tissue, Wnt-3a expression was statistically correlated with the development of metastasis (p = 0.029). CONCLUSIONS: Based on our data, β-catenin may be involved in cancer invasion in pancreatic cancer, and it is not associated with CD44, the invasion related protein. The Korean Association of Internal Medicine 2019-05 2018-10-26 /pmc/articles/PMC6506732/ /pubmed/30360026 http://dx.doi.org/10.3904/kjim.2017.155 Text en Copyright © 2019 The Korean Association of Internal Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nan, Jin Niang Kim, Ok Ran Lee, Myung Ah β-Catenin expression is associated with cell invasiveness in pancreatic cancer |
title | β-Catenin expression is associated with cell invasiveness in pancreatic cancer |
title_full | β-Catenin expression is associated with cell invasiveness in pancreatic cancer |
title_fullStr | β-Catenin expression is associated with cell invasiveness in pancreatic cancer |
title_full_unstemmed | β-Catenin expression is associated with cell invasiveness in pancreatic cancer |
title_short | β-Catenin expression is associated with cell invasiveness in pancreatic cancer |
title_sort | β-catenin expression is associated with cell invasiveness in pancreatic cancer |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506732/ https://www.ncbi.nlm.nih.gov/pubmed/30360026 http://dx.doi.org/10.3904/kjim.2017.155 |
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