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The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice

In order to respond to fluctuating zinc (Zn) in the environment, plants must have a system to control Zn homeostasis. However, how plants maintain an appropriate level of Zn during their growth and development is still poorly understood. In this study, we found that OsHMA3, a tonoplast-localized tra...

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Autores principales: Cai, Hongmei, Huang, Sheng, Che, Jing, Yamaji, Naoki, Ma, Jian Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506766/
https://www.ncbi.nlm.nih.gov/pubmed/30840766
http://dx.doi.org/10.1093/jxb/erz091
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author Cai, Hongmei
Huang, Sheng
Che, Jing
Yamaji, Naoki
Ma, Jian Feng
author_facet Cai, Hongmei
Huang, Sheng
Che, Jing
Yamaji, Naoki
Ma, Jian Feng
author_sort Cai, Hongmei
collection PubMed
description In order to respond to fluctuating zinc (Zn) in the environment, plants must have a system to control Zn homeostasis. However, how plants maintain an appropriate level of Zn during their growth and development is still poorly understood. In this study, we found that OsHMA3, a tonoplast-localized transporter for Zn/Cd, plays an important role in Zn homeostasis in rice. Accessions with the functional allele of OsHMA3 showed greater tolerance to high Zn than those with the non-functional allele based on root elongation test. A (67)Zn-labeling experiment showed that accessions with loss of function of OsHMA3 had lower Zn accumulation in the roots but similar concentrations in the shoots compared with functional OsHMA3 accessions. When exposed to Zn-free growing medium, the concentration in the root cell sap was rapidly decreased in accessions with functional OsHMA3, but less dramatic changes were observed in non-functional accessions. A mobility experiment showed that more Zn in the roots was translocated to the shoots in accessions with functional OsHMA3. Higher expression levels of OsZIP4, OsZIP5, OsZIP8, and OsZIP10 were found in the roots of accessions with functional OsHMA3 in response to Zn deficiency. Taken together, our results indicate that OsHMA3 plays an important role in rice roots in both Zn detoxification and storage by sequestration into the vacuoles, depending on Zn concentration in the environment.
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spelling pubmed-65067662019-05-13 The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice Cai, Hongmei Huang, Sheng Che, Jing Yamaji, Naoki Ma, Jian Feng J Exp Bot Research Papers In order to respond to fluctuating zinc (Zn) in the environment, plants must have a system to control Zn homeostasis. However, how plants maintain an appropriate level of Zn during their growth and development is still poorly understood. In this study, we found that OsHMA3, a tonoplast-localized transporter for Zn/Cd, plays an important role in Zn homeostasis in rice. Accessions with the functional allele of OsHMA3 showed greater tolerance to high Zn than those with the non-functional allele based on root elongation test. A (67)Zn-labeling experiment showed that accessions with loss of function of OsHMA3 had lower Zn accumulation in the roots but similar concentrations in the shoots compared with functional OsHMA3 accessions. When exposed to Zn-free growing medium, the concentration in the root cell sap was rapidly decreased in accessions with functional OsHMA3, but less dramatic changes were observed in non-functional accessions. A mobility experiment showed that more Zn in the roots was translocated to the shoots in accessions with functional OsHMA3. Higher expression levels of OsZIP4, OsZIP5, OsZIP8, and OsZIP10 were found in the roots of accessions with functional OsHMA3 in response to Zn deficiency. Taken together, our results indicate that OsHMA3 plays an important role in rice roots in both Zn detoxification and storage by sequestration into the vacuoles, depending on Zn concentration in the environment. Oxford University Press 2019-05-01 2019-03-06 /pmc/articles/PMC6506766/ /pubmed/30840766 http://dx.doi.org/10.1093/jxb/erz091 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Papers
Cai, Hongmei
Huang, Sheng
Che, Jing
Yamaji, Naoki
Ma, Jian Feng
The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice
title The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice
title_full The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice
title_fullStr The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice
title_full_unstemmed The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice
title_short The tonoplast-localized transporter OsHMA3 plays an important role in maintaining Zn homeostasis in rice
title_sort tonoplast-localized transporter oshma3 plays an important role in maintaining zn homeostasis in rice
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6506766/
https://www.ncbi.nlm.nih.gov/pubmed/30840766
http://dx.doi.org/10.1093/jxb/erz091
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