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In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract

Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance cap...

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Autores principales: Dabas, Deepti, Elias, Ryan J., Ziegler, Gregory R., Lambert, Joshua D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507236/
https://www.ncbi.nlm.nih.gov/pubmed/31179313
http://dx.doi.org/10.1155/2019/6509421
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author Dabas, Deepti
Elias, Ryan J.
Ziegler, Gregory R.
Lambert, Joshua D.
author_facet Dabas, Deepti
Elias, Ryan J.
Ziegler, Gregory R.
Lambert, Joshua D.
author_sort Dabas, Deepti
collection PubMed
description Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D(1) and E(2) in LNCaP cells. This was associated with cell G(0)/G(1) phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models.
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spelling pubmed-65072362019-06-09 In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract Dabas, Deepti Elias, Ryan J. Ziegler, Gregory R. Lambert, Joshua D. Int J Food Sci Research Article Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D(1) and E(2) in LNCaP cells. This was associated with cell G(0)/G(1) phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models. Hindawi 2019-04-24 /pmc/articles/PMC6507236/ /pubmed/31179313 http://dx.doi.org/10.1155/2019/6509421 Text en Copyright © 2019 Deepti Dabas et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dabas, Deepti
Elias, Ryan J.
Ziegler, Gregory R.
Lambert, Joshua D.
In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
title In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
title_full In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
title_fullStr In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
title_full_unstemmed In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
title_short In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
title_sort in vitro antioxidant and cancer inhibitory activity of a colored avocado seed extract
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507236/
https://www.ncbi.nlm.nih.gov/pubmed/31179313
http://dx.doi.org/10.1155/2019/6509421
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