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In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract
Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance cap...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507236/ https://www.ncbi.nlm.nih.gov/pubmed/31179313 http://dx.doi.org/10.1155/2019/6509421 |
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author | Dabas, Deepti Elias, Ryan J. Ziegler, Gregory R. Lambert, Joshua D. |
author_facet | Dabas, Deepti Elias, Ryan J. Ziegler, Gregory R. Lambert, Joshua D. |
author_sort | Dabas, Deepti |
collection | PubMed |
description | Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D(1) and E(2) in LNCaP cells. This was associated with cell G(0)/G(1) phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models. |
format | Online Article Text |
id | pubmed-6507236 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-65072362019-06-09 In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract Dabas, Deepti Elias, Ryan J. Ziegler, Gregory R. Lambert, Joshua D. Int J Food Sci Research Article Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications. Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE). CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg. CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL). CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro. The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h. CASE treatment downregulated the expression of cyclin D(1) and E(2) in LNCaP cells. This was associated with cell G(0)/G(1) phase cycle arrest. CASE also dose-dependently induced apoptosis in LNCaP cells. CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal. Further studies are needed to examine these effects in in vivo models. Hindawi 2019-04-24 /pmc/articles/PMC6507236/ /pubmed/31179313 http://dx.doi.org/10.1155/2019/6509421 Text en Copyright © 2019 Deepti Dabas et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dabas, Deepti Elias, Ryan J. Ziegler, Gregory R. Lambert, Joshua D. In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
title | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
title_full | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
title_fullStr | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
title_full_unstemmed | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
title_short | In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract |
title_sort | in vitro antioxidant and cancer inhibitory activity of a colored avocado seed extract |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507236/ https://www.ncbi.nlm.nih.gov/pubmed/31179313 http://dx.doi.org/10.1155/2019/6509421 |
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