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Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats

Goniothalamin (GTN) is an isolated compound from several plants of the genus Goniothalamus, and its anticancer effect against several cancers was reported. However, there is no scientific data about effects of its higher doses on internal organs. Accordingly, this study aimed to evaluate the acute a...

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Autores principales: Kaid, Fahmi, Alabsi, A. M., Alafifi, Nashwan, Ali-Saeed, Rola, Ameen Al-koshab, May, Ramanathan, Anand, Ali, A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507267/
https://www.ncbi.nlm.nih.gov/pubmed/31178909
http://dx.doi.org/10.1155/2019/6493286
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author Kaid, Fahmi
Alabsi, A. M.
Alafifi, Nashwan
Ali-Saeed, Rola
Ameen Al-koshab, May
Ramanathan, Anand
Ali, A. M.
author_facet Kaid, Fahmi
Alabsi, A. M.
Alafifi, Nashwan
Ali-Saeed, Rola
Ameen Al-koshab, May
Ramanathan, Anand
Ali, A. M.
author_sort Kaid, Fahmi
collection PubMed
description Goniothalamin (GTN) is an isolated compound from several plants of the genus Goniothalamus, and its anticancer effect against several cancers was reported. However, there is no scientific data about effects of its higher doses on internal organs. Accordingly, this study aimed to evaluate the acute and subacute effects of higher doses of GTN on the hematology, biochemistry, and histology of selected internal organs of male Sprague-Dawley rats. In acute study, 35 rats were distributed in 5 groups (n=7) which were intraperitoneally (IP) injected with a single dose of either 100, 200, 300, 400, or 500 mg/kg of GTN, while extra 7 rats serve as a normal control. In subacute study, 7 rats were IP-injected with a daily dose of 42 mg/kg of GTN for 14 days, while another 7 rats serve as a normal control group. The normal controls in both studies were IP-injected simultaneously with 2 ml/kg of 10% DMSO in PBS. At the end of both tests, rats were sacrificed to collect blood for hematology and biochemistry and harvest livers, kidneys, lungs, hearts, spleens, and brains for histology. During acute and subacute exposure, no abnormal changes were observed in the hematology, biochemistry, and histology of the internal organs. However, the 300, 400, and 500 mg/kg of GTN during acute exposure were associated with morbidities and mortalities. Ultimately, GTN could be safe up to the dose of 200 mg/kg, and the dose of 42 mg/kg of GTN was tolerated well.
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spelling pubmed-65072672019-06-09 Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats Kaid, Fahmi Alabsi, A. M. Alafifi, Nashwan Ali-Saeed, Rola Ameen Al-koshab, May Ramanathan, Anand Ali, A. M. J Toxicol Research Article Goniothalamin (GTN) is an isolated compound from several plants of the genus Goniothalamus, and its anticancer effect against several cancers was reported. However, there is no scientific data about effects of its higher doses on internal organs. Accordingly, this study aimed to evaluate the acute and subacute effects of higher doses of GTN on the hematology, biochemistry, and histology of selected internal organs of male Sprague-Dawley rats. In acute study, 35 rats were distributed in 5 groups (n=7) which were intraperitoneally (IP) injected with a single dose of either 100, 200, 300, 400, or 500 mg/kg of GTN, while extra 7 rats serve as a normal control. In subacute study, 7 rats were IP-injected with a daily dose of 42 mg/kg of GTN for 14 days, while another 7 rats serve as a normal control group. The normal controls in both studies were IP-injected simultaneously with 2 ml/kg of 10% DMSO in PBS. At the end of both tests, rats were sacrificed to collect blood for hematology and biochemistry and harvest livers, kidneys, lungs, hearts, spleens, and brains for histology. During acute and subacute exposure, no abnormal changes were observed in the hematology, biochemistry, and histology of the internal organs. However, the 300, 400, and 500 mg/kg of GTN during acute exposure were associated with morbidities and mortalities. Ultimately, GTN could be safe up to the dose of 200 mg/kg, and the dose of 42 mg/kg of GTN was tolerated well. Hindawi 2019-04-23 /pmc/articles/PMC6507267/ /pubmed/31178909 http://dx.doi.org/10.1155/2019/6493286 Text en Copyright © 2019 Fahmi Kaid et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kaid, Fahmi
Alabsi, A. M.
Alafifi, Nashwan
Ali-Saeed, Rola
Ameen Al-koshab, May
Ramanathan, Anand
Ali, A. M.
Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats
title Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats
title_full Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats
title_fullStr Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats
title_full_unstemmed Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats
title_short Histological, Biochemical, and Hematological Effects of Goniothalamin on Selective Internal Organs of Male Sprague-Dawley Rats
title_sort histological, biochemical, and hematological effects of goniothalamin on selective internal organs of male sprague-dawley rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507267/
https://www.ncbi.nlm.nih.gov/pubmed/31178909
http://dx.doi.org/10.1155/2019/6493286
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