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miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1

Liver cancer is a malignant tumor that threatens human health worldwide. It has poor prognosis rates and ineffective therapeutic options. Recently, various miRNAs have been proven to exert promoting or inhibiting functions in different malignancies. However, the definitive mechanisms of miR-99a in l...

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Autores principales: Tao, Changming, Sun, Huiling, Sang, Weiwei, Li, Shanshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507307/
https://www.ncbi.nlm.nih.gov/pubmed/31186723
http://dx.doi.org/10.3892/ol.2019.10199
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author Tao, Changming
Sun, Huiling
Sang, Weiwei
Li, Shanshan
author_facet Tao, Changming
Sun, Huiling
Sang, Weiwei
Li, Shanshan
author_sort Tao, Changming
collection PubMed
description Liver cancer is a malignant tumor that threatens human health worldwide. It has poor prognosis rates and ineffective therapeutic options. Recently, various miRNAs have been proven to exert promoting or inhibiting functions in different malignancies. However, the definitive mechanisms of miR-99a in liver cancer remain unclear. In the current study, we explored the relationships between the expression of miR-99a and HOXA1 in liver cancer tissues and cells to explore their combined effects on the occurrence and metastasis of liver cancer. The expression of miR-99a and HOXA1 in liver cancer tissue samples and cells was measured by RT-qPCR. Following transfection, transwell assays were conducted to assess the invasion and migration capacities of liver cancer cells. Subsequently, western blots and luciferase reporter assays were performed in liver cancer cells to identify the target of miR-99a. The data indicated that miRNA-99a expression was significantly reduced in both liver cancer tissue samples and cells compared with normal tissues and normal liver cells respectively. By contrast, the HOXA1 expression levels in liver cancer tissues and cells were significantly increased in contrast to the control group. The findings also revealed that the miR-99a expression was negatively correlated with HOXA1 expression in liver cancer tissue samples and miR-99a could suppress cell invasion and migration by targeting HOXA1 in liver cancer.
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spelling pubmed-65073072019-06-11 miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1 Tao, Changming Sun, Huiling Sang, Weiwei Li, Shanshan Oncol Lett Articles Liver cancer is a malignant tumor that threatens human health worldwide. It has poor prognosis rates and ineffective therapeutic options. Recently, various miRNAs have been proven to exert promoting or inhibiting functions in different malignancies. However, the definitive mechanisms of miR-99a in liver cancer remain unclear. In the current study, we explored the relationships between the expression of miR-99a and HOXA1 in liver cancer tissues and cells to explore their combined effects on the occurrence and metastasis of liver cancer. The expression of miR-99a and HOXA1 in liver cancer tissue samples and cells was measured by RT-qPCR. Following transfection, transwell assays were conducted to assess the invasion and migration capacities of liver cancer cells. Subsequently, western blots and luciferase reporter assays were performed in liver cancer cells to identify the target of miR-99a. The data indicated that miRNA-99a expression was significantly reduced in both liver cancer tissue samples and cells compared with normal tissues and normal liver cells respectively. By contrast, the HOXA1 expression levels in liver cancer tissues and cells were significantly increased in contrast to the control group. The findings also revealed that the miR-99a expression was negatively correlated with HOXA1 expression in liver cancer tissue samples and miR-99a could suppress cell invasion and migration by targeting HOXA1 in liver cancer. D.A. Spandidos 2019-06 2019-03-29 /pmc/articles/PMC6507307/ /pubmed/31186723 http://dx.doi.org/10.3892/ol.2019.10199 Text en Copyright: © Tao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Tao, Changming
Sun, Huiling
Sang, Weiwei
Li, Shanshan
miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1
title miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1
title_full miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1
title_fullStr miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1
title_full_unstemmed miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1
title_short miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1
title_sort mirna-99a inhibits cell invasion and migration in liver cancer by directly targeting hoxa1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507307/
https://www.ncbi.nlm.nih.gov/pubmed/31186723
http://dx.doi.org/10.3892/ol.2019.10199
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