Small RNA-seq: The RNA 5’-end adapter ligation problem and how to circumvent it

The preparation of small RNA cDNA sequencing libraries depends on the unbiased ligation of adapters to the RNA ends. Small RNA with 5’ recessed ends are poor substrates for enzymatic adapter ligation, but this 5’ adapter ligation problem can go undetected if the library preparation steps are not mon...

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Detalles Bibliográficos
Autores principales: Lama, Lodoe, Cobo, Jose, Buenaventura, Diego, Ryan, Kevin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Journal of Biological Methods 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507418/
https://www.ncbi.nlm.nih.gov/pubmed/31080843
http://dx.doi.org/10.14440/jbm.2019.269

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507418/
https://www.ncbi.nlm.nih.gov/pubmed/31080843
http://dx.doi.org/10.14440/jbm.2019.269

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