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Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway

BACKGROUND: Recent studies have demonstrated that Linc00152 is highly expressed in multiple cancer types and its genes show tumor-promoting characteristics. However, the efficacy and biological mechanism of Linc00152 in bladder cancer remains unclear. MATERIAL/METHODS: We study investigated the rela...

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Autores principales: Xian-li, Tang, Hong, Luo, Hong, Zhou, Yuan, Li, Jun-yong, Dai, Peng, Xian, Yan-ping, Song, Fang, Yuan, Nan, Liu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507494/
https://www.ncbi.nlm.nih.gov/pubmed/31042695
http://dx.doi.org/10.12659/MSM.913944
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author Xian-li, Tang
Hong, Luo
Hong, Zhou
Yuan, Li
Jun-yong, Dai
Peng, Xian
Yan-ping, Song
Fang, Yuan
Nan, Liu
author_facet Xian-li, Tang
Hong, Luo
Hong, Zhou
Yuan, Li
Jun-yong, Dai
Peng, Xian
Yan-ping, Song
Fang, Yuan
Nan, Liu
author_sort Xian-li, Tang
collection PubMed
description BACKGROUND: Recent studies have demonstrated that Linc00152 is highly expressed in multiple cancer types and its genes show tumor-promoting characteristics. However, the efficacy and biological mechanism of Linc00152 in bladder cancer remains unclear. MATERIAL/METHODS: We study investigated the relative expression and promoter methylation of Linc00152 in 126 cases of bladder cancer tissues by qRT-PCR and Bisulfite sequencing PCR. qRT-PCR was used to assess the relative expression of Linc00152 in 4 human bladder cancer cell lines. To explore the biological properties of Linc00152, we performed cell growth and soft-agar colony-formation assays, flow cytometry analyses, wound-healing assay, and Transwell assay. Western blot analysis was used to detect the underlying mechanisms of Linc00152 in bladder cancer. RESULTS: We found that Linc00152 was highly expressed in 126 cases of bladder carcinoma tissues (p<0.001) and 4 cell lines (p<0.01), and Linc00152 is more commonly expressed in patients with advanced-stage cancer (p=0.021). Knockdown of Linc00152 by using siRNAs in bladder cancer cell lines (T24 and HT-1197) suppressed cell viability and growth by causing cell cycle arrest and apoptosis (p<0.001), as well as inhibiting cell migration and invasion (p<0.001). In addition, the quantitative RT-PCR and Western blot results suggest that knockdown of Linc00152 reduced Wnt/β-Catenin signaling (p<0.001). CONCLUSIONS: This research shows that Linc00152 is highly expressed in patients with bladder cancer and the possible carcinogenic effect of Linc00152 in bladder cancer occurs through activating the Wnt/β-Catenin signaling pathway, and could be a new biomarker for diagnosis and prevention of this cancer.
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spelling pubmed-65074942019-05-23 Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway Xian-li, Tang Hong, Luo Hong, Zhou Yuan, Li Jun-yong, Dai Peng, Xian Yan-ping, Song Fang, Yuan Nan, Liu Med Sci Monit Lab/In Vitro Research BACKGROUND: Recent studies have demonstrated that Linc00152 is highly expressed in multiple cancer types and its genes show tumor-promoting characteristics. However, the efficacy and biological mechanism of Linc00152 in bladder cancer remains unclear. MATERIAL/METHODS: We study investigated the relative expression and promoter methylation of Linc00152 in 126 cases of bladder cancer tissues by qRT-PCR and Bisulfite sequencing PCR. qRT-PCR was used to assess the relative expression of Linc00152 in 4 human bladder cancer cell lines. To explore the biological properties of Linc00152, we performed cell growth and soft-agar colony-formation assays, flow cytometry analyses, wound-healing assay, and Transwell assay. Western blot analysis was used to detect the underlying mechanisms of Linc00152 in bladder cancer. RESULTS: We found that Linc00152 was highly expressed in 126 cases of bladder carcinoma tissues (p<0.001) and 4 cell lines (p<0.01), and Linc00152 is more commonly expressed in patients with advanced-stage cancer (p=0.021). Knockdown of Linc00152 by using siRNAs in bladder cancer cell lines (T24 and HT-1197) suppressed cell viability and growth by causing cell cycle arrest and apoptosis (p<0.001), as well as inhibiting cell migration and invasion (p<0.001). In addition, the quantitative RT-PCR and Western blot results suggest that knockdown of Linc00152 reduced Wnt/β-Catenin signaling (p<0.001). CONCLUSIONS: This research shows that Linc00152 is highly expressed in patients with bladder cancer and the possible carcinogenic effect of Linc00152 in bladder cancer occurs through activating the Wnt/β-Catenin signaling pathway, and could be a new biomarker for diagnosis and prevention of this cancer. International Scientific Literature, Inc. 2019-05-01 /pmc/articles/PMC6507494/ /pubmed/31042695 http://dx.doi.org/10.12659/MSM.913944 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Xian-li, Tang
Hong, Luo
Hong, Zhou
Yuan, Li
Jun-yong, Dai
Peng, Xian
Yan-ping, Song
Fang, Yuan
Nan, Liu
Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway
title Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway
title_full Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway
title_fullStr Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway
title_full_unstemmed Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway
title_short Higher Expression of Linc00152 Promotes Bladder Cancer Proliferation and Metastasis by Activating the Wnt/β-Catenin Signaling Pathway
title_sort higher expression of linc00152 promotes bladder cancer proliferation and metastasis by activating the wnt/β-catenin signaling pathway
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507494/
https://www.ncbi.nlm.nih.gov/pubmed/31042695
http://dx.doi.org/10.12659/MSM.913944
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