Cargando…

Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications

Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- and multiple-species oral biofilms in real time through impedance technology, which elucidates the kinetics of biofilm formation in 96-well plates, without the requirement for any further manipul...

Descripción completa

Detalles Bibliográficos
Autores principales: Mira, Alex, Buetas, Elena, Rosier, Bob, Mazurel, Danuta, Villanueva-Castellote, Álvaro, Llena, Carmen, Ferrer, Maria D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507917/
https://www.ncbi.nlm.nih.gov/pubmed/31105900
http://dx.doi.org/10.1080/20002297.2019.1609838
_version_ 1783417067555258368
author Mira, Alex
Buetas, Elena
Rosier, Bob
Mazurel, Danuta
Villanueva-Castellote, Álvaro
Llena, Carmen
Ferrer, Maria D.
author_facet Mira, Alex
Buetas, Elena
Rosier, Bob
Mazurel, Danuta
Villanueva-Castellote, Álvaro
Llena, Carmen
Ferrer, Maria D.
author_sort Mira, Alex
collection PubMed
description Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- and multiple-species oral biofilms in real time through impedance technology, which elucidates the kinetics of biofilm formation in 96-well plates, without the requirement for any further manipulation. Design and Results: Using this system, biofilms of Streptococcus mutans appear to be sugar-dependent and highly resistant to amoxicilin, an antibiotic to which this oral pathogen is highly sensitive in a planktonic state. Saliva, tongue and dental plaque samples were also used as inocula to form multiple-species biofilms. DNA isolation and Illumina sequencing of the biofilms showed that the multi-species biofilms were formed by tens or hundreds of species, had a similar composition to the original inoculum, and included fastidious microorganisms which are important for oral health and disease. As an example of the potential applications of the model, we show that oral biofilms can be inhibited by amoxicilin, but in some cases they are induced by the antibiotic, suggesting the existence of responders and non-responders to a given antibiotic. Conclusions: We therefore propose the system as a valid in vitro model to study oral biofilm dynamics, including their susceptibility to antibiotics, antiseptics or anti-adhesive compounds.
format Online
Article
Text
id pubmed-6507917
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-65079172019-05-17 Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications Mira, Alex Buetas, Elena Rosier, Bob Mazurel, Danuta Villanueva-Castellote, Álvaro Llena, Carmen Ferrer, Maria D. J Oral Microbiol Original Article Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- and multiple-species oral biofilms in real time through impedance technology, which elucidates the kinetics of biofilm formation in 96-well plates, without the requirement for any further manipulation. Design and Results: Using this system, biofilms of Streptococcus mutans appear to be sugar-dependent and highly resistant to amoxicilin, an antibiotic to which this oral pathogen is highly sensitive in a planktonic state. Saliva, tongue and dental plaque samples were also used as inocula to form multiple-species biofilms. DNA isolation and Illumina sequencing of the biofilms showed that the multi-species biofilms were formed by tens or hundreds of species, had a similar composition to the original inoculum, and included fastidious microorganisms which are important for oral health and disease. As an example of the potential applications of the model, we show that oral biofilms can be inhibited by amoxicilin, but in some cases they are induced by the antibiotic, suggesting the existence of responders and non-responders to a given antibiotic. Conclusions: We therefore propose the system as a valid in vitro model to study oral biofilm dynamics, including their susceptibility to antibiotics, antiseptics or anti-adhesive compounds. Taylor & Francis 2019-05-06 /pmc/articles/PMC6507917/ /pubmed/31105900 http://dx.doi.org/10.1080/20002297.2019.1609838 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Mira, Alex
Buetas, Elena
Rosier, Bob
Mazurel, Danuta
Villanueva-Castellote, Álvaro
Llena, Carmen
Ferrer, Maria D.
Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
title Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
title_full Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
title_fullStr Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
title_full_unstemmed Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
title_short Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
title_sort development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507917/
https://www.ncbi.nlm.nih.gov/pubmed/31105900
http://dx.doi.org/10.1080/20002297.2019.1609838
work_keys_str_mv AT miraalex developmentofaninvitrosystemtostudyoralbiofilmsinrealtimethroughimpedancetechnologyvalidationandpotentialapplications
AT buetaselena developmentofaninvitrosystemtostudyoralbiofilmsinrealtimethroughimpedancetechnologyvalidationandpotentialapplications
AT rosierbob developmentofaninvitrosystemtostudyoralbiofilmsinrealtimethroughimpedancetechnologyvalidationandpotentialapplications
AT mazureldanuta developmentofaninvitrosystemtostudyoralbiofilmsinrealtimethroughimpedancetechnologyvalidationandpotentialapplications
AT villanuevacastellotealvaro developmentofaninvitrosystemtostudyoralbiofilmsinrealtimethroughimpedancetechnologyvalidationandpotentialapplications
AT llenacarmen developmentofaninvitrosystemtostudyoralbiofilmsinrealtimethroughimpedancetechnologyvalidationandpotentialapplications
AT ferrermariad developmentofaninvitrosystemtostudyoralbiofilmsinrealtimethroughimpedancetechnologyvalidationandpotentialapplications