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Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications
Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- and multiple-species oral biofilms in real time through impedance technology, which elucidates the kinetics of biofilm formation in 96-well plates, without the requirement for any further manipul...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507917/ https://www.ncbi.nlm.nih.gov/pubmed/31105900 http://dx.doi.org/10.1080/20002297.2019.1609838 |
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author | Mira, Alex Buetas, Elena Rosier, Bob Mazurel, Danuta Villanueva-Castellote, Álvaro Llena, Carmen Ferrer, Maria D. |
author_facet | Mira, Alex Buetas, Elena Rosier, Bob Mazurel, Danuta Villanueva-Castellote, Álvaro Llena, Carmen Ferrer, Maria D. |
author_sort | Mira, Alex |
collection | PubMed |
description | Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- and multiple-species oral biofilms in real time through impedance technology, which elucidates the kinetics of biofilm formation in 96-well plates, without the requirement for any further manipulation. Design and Results: Using this system, biofilms of Streptococcus mutans appear to be sugar-dependent and highly resistant to amoxicilin, an antibiotic to which this oral pathogen is highly sensitive in a planktonic state. Saliva, tongue and dental plaque samples were also used as inocula to form multiple-species biofilms. DNA isolation and Illumina sequencing of the biofilms showed that the multi-species biofilms were formed by tens or hundreds of species, had a similar composition to the original inoculum, and included fastidious microorganisms which are important for oral health and disease. As an example of the potential applications of the model, we show that oral biofilms can be inhibited by amoxicilin, but in some cases they are induced by the antibiotic, suggesting the existence of responders and non-responders to a given antibiotic. Conclusions: We therefore propose the system as a valid in vitro model to study oral biofilm dynamics, including their susceptibility to antibiotics, antiseptics or anti-adhesive compounds. |
format | Online Article Text |
id | pubmed-6507917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-65079172019-05-17 Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications Mira, Alex Buetas, Elena Rosier, Bob Mazurel, Danuta Villanueva-Castellote, Álvaro Llena, Carmen Ferrer, Maria D. J Oral Microbiol Original Article Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- and multiple-species oral biofilms in real time through impedance technology, which elucidates the kinetics of biofilm formation in 96-well plates, without the requirement for any further manipulation. Design and Results: Using this system, biofilms of Streptococcus mutans appear to be sugar-dependent and highly resistant to amoxicilin, an antibiotic to which this oral pathogen is highly sensitive in a planktonic state. Saliva, tongue and dental plaque samples were also used as inocula to form multiple-species biofilms. DNA isolation and Illumina sequencing of the biofilms showed that the multi-species biofilms were formed by tens or hundreds of species, had a similar composition to the original inoculum, and included fastidious microorganisms which are important for oral health and disease. As an example of the potential applications of the model, we show that oral biofilms can be inhibited by amoxicilin, but in some cases they are induced by the antibiotic, suggesting the existence of responders and non-responders to a given antibiotic. Conclusions: We therefore propose the system as a valid in vitro model to study oral biofilm dynamics, including their susceptibility to antibiotics, antiseptics or anti-adhesive compounds. Taylor & Francis 2019-05-06 /pmc/articles/PMC6507917/ /pubmed/31105900 http://dx.doi.org/10.1080/20002297.2019.1609838 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Mira, Alex Buetas, Elena Rosier, Bob Mazurel, Danuta Villanueva-Castellote, Álvaro Llena, Carmen Ferrer, Maria D. Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications |
title | Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications |
title_full | Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications |
title_fullStr | Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications |
title_full_unstemmed | Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications |
title_short | Development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications |
title_sort | development of an in vitro system to study oral biofilms in real time through impedance technology: validation and potential applications |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6507917/ https://www.ncbi.nlm.nih.gov/pubmed/31105900 http://dx.doi.org/10.1080/20002297.2019.1609838 |
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