Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to...

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Autores principales: Gwynne, Lauren, Sedgwick, Adam C., Gardiner, Jordan E., Williams, George T., Kim, Gyoungmi, Lowe, John P., Maillard, Jean-Yves, Jenkins, A. Toby A., Bull, Steven D., Sessler, Jonathan L., Yoon, Juyoung, James, Tony D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508040/
https://www.ncbi.nlm.nih.gov/pubmed/31119120
http://dx.doi.org/10.3389/fchem.2019.00255
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author Gwynne, Lauren
Sedgwick, Adam C.
Gardiner, Jordan E.
Williams, George T.
Kim, Gyoungmi
Lowe, John P.
Maillard, Jean-Yves
Jenkins, A. Toby A.
Bull, Steven D.
Sessler, Jonathan L.
Yoon, Juyoung
James, Tony D.
author_facet Gwynne, Lauren
Sedgwick, Adam C.
Gardiner, Jordan E.
Williams, George T.
Kim, Gyoungmi
Lowe, John P.
Maillard, Jean-Yves
Jenkins, A. Toby A.
Bull, Steven D.
Sessler, Jonathan L.
Yoon, Juyoung
James, Tony D.
author_sort Gwynne, Lauren
collection PubMed
description A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
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spelling pubmed-65080402019-05-22 Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells Gwynne, Lauren Sedgwick, Adam C. Gardiner, Jordan E. Williams, George T. Kim, Gyoungmi Lowe, John P. Maillard, Jean-Yves Jenkins, A. Toby A. Bull, Steven D. Sessler, Jonathan L. Yoon, Juyoung James, Tony D. Front Chem Chemistry A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems. Frontiers Media S.A. 2019-04-30 /pmc/articles/PMC6508040/ /pubmed/31119120 http://dx.doi.org/10.3389/fchem.2019.00255 Text en Copyright © 2019 Gwynne, Sedgwick, Gardiner, Williams, Kim, Lowe, Maillard, Jenkins, Bull, Sessler, Yoon and James. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Gwynne, Lauren
Sedgwick, Adam C.
Gardiner, Jordan E.
Williams, George T.
Kim, Gyoungmi
Lowe, John P.
Maillard, Jean-Yves
Jenkins, A. Toby A.
Bull, Steven D.
Sessler, Jonathan L.
Yoon, Juyoung
James, Tony D.
Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_full Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_fullStr Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_full_unstemmed Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_short Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_sort long wavelength tcf-based fluorescent probe for the detection of alkaline phosphatase in live cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508040/
https://www.ncbi.nlm.nih.gov/pubmed/31119120
http://dx.doi.org/10.3389/fchem.2019.00255
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