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NanoR: A user-friendly R package to analyze and compare nanopore sequencing data

MinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and portable sequencing device and, thanks to its unique properties, is becoming more and more popular among biologists; on the other, Gri...

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Autores principales: Bolognini, Davide, Bartalucci, Niccolò, Mingrino, Alessandra, Vannucchi, Alessandro Maria, Magi, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508625/
https://www.ncbi.nlm.nih.gov/pubmed/31071140
http://dx.doi.org/10.1371/journal.pone.0216471
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author Bolognini, Davide
Bartalucci, Niccolò
Mingrino, Alessandra
Vannucchi, Alessandro Maria
Magi, Alberto
author_facet Bolognini, Davide
Bartalucci, Niccolò
Mingrino, Alessandra
Vannucchi, Alessandro Maria
Magi, Alberto
author_sort Bolognini, Davide
collection PubMed
description MinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and portable sequencing device and, thanks to its unique properties, is becoming more and more popular among biologists; on the other, GridION X5, mainly for its costs, is less widespread but highly suitable for researchers with large sequencing projects. Despite the fact that Oxford Nanopore Technologies’ devices have been increasingly used in the last few years, there is a lack of high-performing and user-friendly tools to handle the data outputted by both MinION and GridION X5 platforms. Here we present NanoR, a cross-platform R package designed with the purpose to simplify and improve nanopore data visualization. Indeed, NanoR is built on few functions but overcomes the capabilities of existing tools to extract meaningful informations from MinION sequencing data; in addition, as exclusive features, NanoR can deal with GridION X5 sequencing outputs and allows comparison of both MinION and GridION X5 sequencing data in one command. NanoR is released as free package for R at https://github.com/davidebolo1993/NanoR.
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spelling pubmed-65086252019-05-23 NanoR: A user-friendly R package to analyze and compare nanopore sequencing data Bolognini, Davide Bartalucci, Niccolò Mingrino, Alessandra Vannucchi, Alessandro Maria Magi, Alberto PLoS One Research Article MinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and portable sequencing device and, thanks to its unique properties, is becoming more and more popular among biologists; on the other, GridION X5, mainly for its costs, is less widespread but highly suitable for researchers with large sequencing projects. Despite the fact that Oxford Nanopore Technologies’ devices have been increasingly used in the last few years, there is a lack of high-performing and user-friendly tools to handle the data outputted by both MinION and GridION X5 platforms. Here we present NanoR, a cross-platform R package designed with the purpose to simplify and improve nanopore data visualization. Indeed, NanoR is built on few functions but overcomes the capabilities of existing tools to extract meaningful informations from MinION sequencing data; in addition, as exclusive features, NanoR can deal with GridION X5 sequencing outputs and allows comparison of both MinION and GridION X5 sequencing data in one command. NanoR is released as free package for R at https://github.com/davidebolo1993/NanoR. Public Library of Science 2019-05-09 /pmc/articles/PMC6508625/ /pubmed/31071140 http://dx.doi.org/10.1371/journal.pone.0216471 Text en © 2019 Bolognini et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bolognini, Davide
Bartalucci, Niccolò
Mingrino, Alessandra
Vannucchi, Alessandro Maria
Magi, Alberto
NanoR: A user-friendly R package to analyze and compare nanopore sequencing data
title NanoR: A user-friendly R package to analyze and compare nanopore sequencing data
title_full NanoR: A user-friendly R package to analyze and compare nanopore sequencing data
title_fullStr NanoR: A user-friendly R package to analyze and compare nanopore sequencing data
title_full_unstemmed NanoR: A user-friendly R package to analyze and compare nanopore sequencing data
title_short NanoR: A user-friendly R package to analyze and compare nanopore sequencing data
title_sort nanor: a user-friendly r package to analyze and compare nanopore sequencing data
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508625/
https://www.ncbi.nlm.nih.gov/pubmed/31071140
http://dx.doi.org/10.1371/journal.pone.0216471
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