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NanoR: A user-friendly R package to analyze and compare nanopore sequencing data
MinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and portable sequencing device and, thanks to its unique properties, is becoming more and more popular among biologists; on the other, Gri...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508625/ https://www.ncbi.nlm.nih.gov/pubmed/31071140 http://dx.doi.org/10.1371/journal.pone.0216471 |
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author | Bolognini, Davide Bartalucci, Niccolò Mingrino, Alessandra Vannucchi, Alessandro Maria Magi, Alberto |
author_facet | Bolognini, Davide Bartalucci, Niccolò Mingrino, Alessandra Vannucchi, Alessandro Maria Magi, Alberto |
author_sort | Bolognini, Davide |
collection | PubMed |
description | MinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and portable sequencing device and, thanks to its unique properties, is becoming more and more popular among biologists; on the other, GridION X5, mainly for its costs, is less widespread but highly suitable for researchers with large sequencing projects. Despite the fact that Oxford Nanopore Technologies’ devices have been increasingly used in the last few years, there is a lack of high-performing and user-friendly tools to handle the data outputted by both MinION and GridION X5 platforms. Here we present NanoR, a cross-platform R package designed with the purpose to simplify and improve nanopore data visualization. Indeed, NanoR is built on few functions but overcomes the capabilities of existing tools to extract meaningful informations from MinION sequencing data; in addition, as exclusive features, NanoR can deal with GridION X5 sequencing outputs and allows comparison of both MinION and GridION X5 sequencing data in one command. NanoR is released as free package for R at https://github.com/davidebolo1993/NanoR. |
format | Online Article Text |
id | pubmed-6508625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65086252019-05-23 NanoR: A user-friendly R package to analyze and compare nanopore sequencing data Bolognini, Davide Bartalucci, Niccolò Mingrino, Alessandra Vannucchi, Alessandro Maria Magi, Alberto PLoS One Research Article MinION and GridION X5 from Oxford Nanopore Technologies are devices for real-time DNA and RNA sequencing. On the one hand, MinION is the only real-time, low cost and portable sequencing device and, thanks to its unique properties, is becoming more and more popular among biologists; on the other, GridION X5, mainly for its costs, is less widespread but highly suitable for researchers with large sequencing projects. Despite the fact that Oxford Nanopore Technologies’ devices have been increasingly used in the last few years, there is a lack of high-performing and user-friendly tools to handle the data outputted by both MinION and GridION X5 platforms. Here we present NanoR, a cross-platform R package designed with the purpose to simplify and improve nanopore data visualization. Indeed, NanoR is built on few functions but overcomes the capabilities of existing tools to extract meaningful informations from MinION sequencing data; in addition, as exclusive features, NanoR can deal with GridION X5 sequencing outputs and allows comparison of both MinION and GridION X5 sequencing data in one command. NanoR is released as free package for R at https://github.com/davidebolo1993/NanoR. Public Library of Science 2019-05-09 /pmc/articles/PMC6508625/ /pubmed/31071140 http://dx.doi.org/10.1371/journal.pone.0216471 Text en © 2019 Bolognini et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bolognini, Davide Bartalucci, Niccolò Mingrino, Alessandra Vannucchi, Alessandro Maria Magi, Alberto NanoR: A user-friendly R package to analyze and compare nanopore sequencing data |
title | NanoR: A user-friendly R package to analyze and compare nanopore sequencing data |
title_full | NanoR: A user-friendly R package to analyze and compare nanopore sequencing data |
title_fullStr | NanoR: A user-friendly R package to analyze and compare nanopore sequencing data |
title_full_unstemmed | NanoR: A user-friendly R package to analyze and compare nanopore sequencing data |
title_short | NanoR: A user-friendly R package to analyze and compare nanopore sequencing data |
title_sort | nanor: a user-friendly r package to analyze and compare nanopore sequencing data |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508625/ https://www.ncbi.nlm.nih.gov/pubmed/31071140 http://dx.doi.org/10.1371/journal.pone.0216471 |
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