qPCR assays to quantitate tRNApyl and pylRS expression in engineered cell lines

Non-natural amino acids (nnAA) contain unique functional moieties that greatly expand the available tool set for protein engineering. But incorporation of nnAAs requires the function of an orthogonal aminoacyl tRNA synthetase/tRNA pair. Stable cell lines expressing these components have been shown c...

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Detalles Bibliográficos
Autores principales: Garcia, Andrew, Roy, Gargi, Kiefer, Christine, Wilson, Susan, Marelli, Marcello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508675/
https://www.ncbi.nlm.nih.gov/pubmed/31071154
http://dx.doi.org/10.1371/journal.pone.0216356

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508675/
https://www.ncbi.nlm.nih.gov/pubmed/31071154
http://dx.doi.org/10.1371/journal.pone.0216356

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