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Validation of use of the miniPCR thermocycler for Ebola and Zika virus detection

The development of point-of-care (POC) diagnostic systems has received well-deserved attention in recent years in the scientific literature, and many experimental systems show great promise in real settings. However, in the case of an epidemic emergency (or a natural disaster), the first line of res...

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Detalles Bibliográficos
Autores principales: González-González, Everardo, Mendoza-Ramos, Jackelin Lizeth, Pedroza, Sara Cristina, Cuellar-Monterrubio, Aimé Alexandra, Márquez-Ipiña, Alan Roberto, Lira-Serhan, Daniel, Trujillo-de Santiago, Grissel, Alvarez, Mario Moisés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508694/
https://www.ncbi.nlm.nih.gov/pubmed/31071117
http://dx.doi.org/10.1371/journal.pone.0215642
Descripción
Sumario:The development of point-of-care (POC) diagnostic systems has received well-deserved attention in recent years in the scientific literature, and many experimental systems show great promise in real settings. However, in the case of an epidemic emergency (or a natural disaster), the first line of response should be based on commercially available and validated resources. Here, we compare the performance and ease of use of the miniPCR, a recently commercially available compact and portable PCR device, and a conventional thermocycler for the diagnostics of viral nucleic acids. We used both thermocyclers to detect and amplify Ebola and Zika DNA sequences of different lengths (in the range of 91 to 300 nucleotides) at different concentrations (in the range of ~50 to 4.0 x 10(8) DNA copies). Our results suggest that the performance of both thermocyclers is quite similar. Moreover, the portability, ease of use, and reproducibility of the miniPCR makes it a reliable alternative for point-of-care nucleic acid detection and amplification.