Splice donor site sgRNAs enhance CRISPR/Cas9-mediated knockout efficiency

CRISPR/Cas9 allows the generation of knockout cell lines and null zygotes by inducing site-specific double-stranded breaks. In most cases the DSB is repaired by non-homologous end joining, resulting in small nucleotide insertions or deletions that can be used to construct knockout alleles. However,...

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Detalles Bibliográficos
Autores principales: García-Tuñón, Ignacio, Alonso-Pérez, Verónica, Vuelta, Elena, Pérez- Ramos, Sandra, Herrero, María, Méndez, Lucía, Hernández-Sánchez, Jesús María, Martín-Izquierdo, Marta, Saldaña, Raquel, Sevilla, Julián, Sánchez- Guijo, Fermín, Hernández-Rivas, Jesús María, Sánchez-Martín, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508695/
https://www.ncbi.nlm.nih.gov/pubmed/31071190
http://dx.doi.org/10.1371/journal.pone.0216674

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