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An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion

Nectar is the main reward that flowers offer to pollinators to entice repeated visitation. Cucurbita pepo (squash) is an excellent model for studying nectar biology, as it has large nectaries that produce large volumes of nectar relative to most other species. Squash is also monoecious, having both...

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Autores principales: Solhaug, Erik M., Roy, Rahul, Chatt, Elizabeth C., Klinkenberg, Peter M., Mohd‐Fadzil, Nur‐Aziatull, Hampton, Marshall, Nikolau, Basil J., Carter, Clay J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508809/
https://www.ncbi.nlm.nih.gov/pubmed/31245763
http://dx.doi.org/10.1002/pld3.120
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author Solhaug, Erik M.
Roy, Rahul
Chatt, Elizabeth C.
Klinkenberg, Peter M.
Mohd‐Fadzil, Nur‐Aziatull
Hampton, Marshall
Nikolau, Basil J.
Carter, Clay J.
author_facet Solhaug, Erik M.
Roy, Rahul
Chatt, Elizabeth C.
Klinkenberg, Peter M.
Mohd‐Fadzil, Nur‐Aziatull
Hampton, Marshall
Nikolau, Basil J.
Carter, Clay J.
author_sort Solhaug, Erik M.
collection PubMed
description Nectar is the main reward that flowers offer to pollinators to entice repeated visitation. Cucurbita pepo (squash) is an excellent model for studying nectar biology, as it has large nectaries that produce large volumes of nectar relative to most other species. Squash is also monoecious, having both female and male flowers on the same plant, which allows comparative analyses of nectary function in one individual. Here, we report the nectary transcriptomes from both female and male nectaries at four stages of floral maturation. Analysis of these transcriptomes and subsequent confirmatory experiments revealed a metabolic progression in nectaries leading from starch synthesis to starch degradation and to sucrose biosynthesis. These results are consistent with previously published models of nectar secretion and also suggest how a sucrose‐rich nectar can be synthesized and secreted in the absence of active transport across the plasma membrane. Nontargeted metabolomic analyses of nectars also confidently identified 40 metabolites in both female and male nectars, with some displaying preferential accumulation in nectar of either male or female flowers. Cumulatively, this study identified gene targets for reverse genetics approaches to study nectary function, as well as previously unreported nectar metabolites that may function in plant‐biotic interactions.
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spelling pubmed-65088092019-06-26 An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion Solhaug, Erik M. Roy, Rahul Chatt, Elizabeth C. Klinkenberg, Peter M. Mohd‐Fadzil, Nur‐Aziatull Hampton, Marshall Nikolau, Basil J. Carter, Clay J. Plant Direct Original Research Nectar is the main reward that flowers offer to pollinators to entice repeated visitation. Cucurbita pepo (squash) is an excellent model for studying nectar biology, as it has large nectaries that produce large volumes of nectar relative to most other species. Squash is also monoecious, having both female and male flowers on the same plant, which allows comparative analyses of nectary function in one individual. Here, we report the nectary transcriptomes from both female and male nectaries at four stages of floral maturation. Analysis of these transcriptomes and subsequent confirmatory experiments revealed a metabolic progression in nectaries leading from starch synthesis to starch degradation and to sucrose biosynthesis. These results are consistent with previously published models of nectar secretion and also suggest how a sucrose‐rich nectar can be synthesized and secreted in the absence of active transport across the plasma membrane. Nontargeted metabolomic analyses of nectars also confidently identified 40 metabolites in both female and male nectars, with some displaying preferential accumulation in nectar of either male or female flowers. Cumulatively, this study identified gene targets for reverse genetics approaches to study nectary function, as well as previously unreported nectar metabolites that may function in plant‐biotic interactions. John Wiley and Sons Inc. 2019-02-28 /pmc/articles/PMC6508809/ /pubmed/31245763 http://dx.doi.org/10.1002/pld3.120 Text en © 2019 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Solhaug, Erik M.
Roy, Rahul
Chatt, Elizabeth C.
Klinkenberg, Peter M.
Mohd‐Fadzil, Nur‐Aziatull
Hampton, Marshall
Nikolau, Basil J.
Carter, Clay J.
An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion
title An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion
title_full An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion
title_fullStr An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion
title_full_unstemmed An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion
title_short An integrated transcriptomics and metabolomics analysis of the Cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion
title_sort integrated transcriptomics and metabolomics analysis of the cucurbita pepo nectary implicates key modules of primary metabolism involved in nectar synthesis and secretion
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508809/
https://www.ncbi.nlm.nih.gov/pubmed/31245763
http://dx.doi.org/10.1002/pld3.120
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