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The CURE for Cultivating Fastidious Microbes
Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Society of Microbiology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508911/ https://www.ncbi.nlm.nih.gov/pubmed/31160939 http://dx.doi.org/10.1128/jmbe.v20i1.1635 |
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author | Bakshi, Arundhati Webber, Austen T. Patrick, Lorelei E. Wischusen, William Thrash, Cameron |
author_facet | Bakshi, Arundhati Webber, Austen T. Patrick, Lorelei E. Wischusen, William Thrash, Cameron |
author_sort | Bakshi, Arundhati |
collection | PubMed |
description | Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-to-extinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the mCUREs achieved similar cultivability success as implementation of the protocol in a standard laboratory setting. Our modular framework facilitates customization of the curriculum for use in multiple settings and we provide classroom exercises, assignments, assessment tools, and examples of student output to assist with implementation. |
format | Online Article Text |
id | pubmed-6508911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-65089112019-06-03 The CURE for Cultivating Fastidious Microbes Bakshi, Arundhati Webber, Austen T. Patrick, Lorelei E. Wischusen, William Thrash, Cameron J Microbiol Biol Educ Curriculum Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-to-extinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the mCUREs achieved similar cultivability success as implementation of the protocol in a standard laboratory setting. Our modular framework facilitates customization of the curriculum for use in multiple settings and we provide classroom exercises, assignments, assessment tools, and examples of student output to assist with implementation. American Society of Microbiology 2019-04-26 /pmc/articles/PMC6508911/ /pubmed/31160939 http://dx.doi.org/10.1128/jmbe.v20i1.1635 Text en ©2019 Author(s). Published by the American Society for Microbiology This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work. |
spellingShingle | Curriculum Bakshi, Arundhati Webber, Austen T. Patrick, Lorelei E. Wischusen, William Thrash, Cameron The CURE for Cultivating Fastidious Microbes |
title | The CURE for Cultivating Fastidious Microbes |
title_full | The CURE for Cultivating Fastidious Microbes |
title_fullStr | The CURE for Cultivating Fastidious Microbes |
title_full_unstemmed | The CURE for Cultivating Fastidious Microbes |
title_short | The CURE for Cultivating Fastidious Microbes |
title_sort | cure for cultivating fastidious microbes |
topic | Curriculum |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6508911/ https://www.ncbi.nlm.nih.gov/pubmed/31160939 http://dx.doi.org/10.1128/jmbe.v20i1.1635 |
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