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Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library

The inferior olive (IO) is an evolutionarily conserved brain stem structure and its output activity plays a major role in the cerebellar computation necessary for controlling the temporal accuracy of motor behavior. The precise timing and synchronization of IO network activity has been attributed to...

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Autores principales: Vrieler, Nora, Loyola, Sebastian, Yarden-Rabinowitz, Yasmin, Hoogendorp, Jesse, Medvedev, Nikolay, Hoogland, Tycho M., De Zeeuw, Chris I., De Schutter, Erik, Yarom, Yosef, Negrello, Mario, Torben-Nielsen, Ben, Uusisaari, Marylka Yoe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509097/
https://www.ncbi.nlm.nih.gov/pubmed/30929054
http://dx.doi.org/10.1007/s00429-019-01859-z
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author Vrieler, Nora
Loyola, Sebastian
Yarden-Rabinowitz, Yasmin
Hoogendorp, Jesse
Medvedev, Nikolay
Hoogland, Tycho M.
De Zeeuw, Chris I.
De Schutter, Erik
Yarom, Yosef
Negrello, Mario
Torben-Nielsen, Ben
Uusisaari, Marylka Yoe
author_facet Vrieler, Nora
Loyola, Sebastian
Yarden-Rabinowitz, Yasmin
Hoogendorp, Jesse
Medvedev, Nikolay
Hoogland, Tycho M.
De Zeeuw, Chris I.
De Schutter, Erik
Yarom, Yosef
Negrello, Mario
Torben-Nielsen, Ben
Uusisaari, Marylka Yoe
author_sort Vrieler, Nora
collection PubMed
description The inferior olive (IO) is an evolutionarily conserved brain stem structure and its output activity plays a major role in the cerebellar computation necessary for controlling the temporal accuracy of motor behavior. The precise timing and synchronization of IO network activity has been attributed to the dendro-dendritic gap junctions mediating electrical coupling within the IO nucleus. Thus, the dendritic morphology and spatial arrangement of IO neurons governs how synchronized activity emerges in this nucleus. To date, IO neuron structural properties have been characterized in few studies and with small numbers of neurons; these investigations have described IO neurons as belonging to two morphologically distinct types, “curly” and “straight”. In this work we collect a large number of individual IO neuron morphologies visualized using different labeling techniques and present a thorough examination of their morphological properties and spatial arrangement within the olivary neuropil. Our results show that the extensive heterogeneity in IO neuron dendritic morphologies occupies a continuous range between the classically described “curly” and “straight” types, and that this continuum is well represented by a relatively simple measure of “straightness”. Furthermore, we find that IO neuron dendritic trees are often directionally oriented. Combined with an examination of cell body density distributions and dendritic orientation of adjacent IO neurons, our results suggest that the IO network may be organized into groups of densely coupled neurons interspersed with areas of weaker coupling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-019-01859-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-65090972019-05-28 Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library Vrieler, Nora Loyola, Sebastian Yarden-Rabinowitz, Yasmin Hoogendorp, Jesse Medvedev, Nikolay Hoogland, Tycho M. De Zeeuw, Chris I. De Schutter, Erik Yarom, Yosef Negrello, Mario Torben-Nielsen, Ben Uusisaari, Marylka Yoe Brain Struct Funct Original Article The inferior olive (IO) is an evolutionarily conserved brain stem structure and its output activity plays a major role in the cerebellar computation necessary for controlling the temporal accuracy of motor behavior. The precise timing and synchronization of IO network activity has been attributed to the dendro-dendritic gap junctions mediating electrical coupling within the IO nucleus. Thus, the dendritic morphology and spatial arrangement of IO neurons governs how synchronized activity emerges in this nucleus. To date, IO neuron structural properties have been characterized in few studies and with small numbers of neurons; these investigations have described IO neurons as belonging to two morphologically distinct types, “curly” and “straight”. In this work we collect a large number of individual IO neuron morphologies visualized using different labeling techniques and present a thorough examination of their morphological properties and spatial arrangement within the olivary neuropil. Our results show that the extensive heterogeneity in IO neuron dendritic morphologies occupies a continuous range between the classically described “curly” and “straight” types, and that this continuum is well represented by a relatively simple measure of “straightness”. Furthermore, we find that IO neuron dendritic trees are often directionally oriented. Combined with an examination of cell body density distributions and dendritic orientation of adjacent IO neurons, our results suggest that the IO network may be organized into groups of densely coupled neurons interspersed with areas of weaker coupling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00429-019-01859-z) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-03-30 2019 /pmc/articles/PMC6509097/ /pubmed/30929054 http://dx.doi.org/10.1007/s00429-019-01859-z Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Vrieler, Nora
Loyola, Sebastian
Yarden-Rabinowitz, Yasmin
Hoogendorp, Jesse
Medvedev, Nikolay
Hoogland, Tycho M.
De Zeeuw, Chris I.
De Schutter, Erik
Yarom, Yosef
Negrello, Mario
Torben-Nielsen, Ben
Uusisaari, Marylka Yoe
Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library
title Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library
title_full Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library
title_fullStr Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library
title_full_unstemmed Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library
title_short Variability and directionality of inferior olive neuron dendrites revealed by detailed 3D characterization of an extensive morphological library
title_sort variability and directionality of inferior olive neuron dendrites revealed by detailed 3d characterization of an extensive morphological library
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509097/
https://www.ncbi.nlm.nih.gov/pubmed/30929054
http://dx.doi.org/10.1007/s00429-019-01859-z
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