MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market

In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-tim...

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Autores principales: Fraiture, Marie-Alice, Ujhelyi, Gabriella, Ovesná, Jaroslava, Van Geel, Dirk, De Keersmaecker, Sigrid, Saltykova, Assia, Papazova, Nina, Roosens, Nancy H. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509135/
https://www.ncbi.nlm.nih.gov/pubmed/31073231
http://dx.doi.org/10.1038/s41598-019-43463-5
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author Fraiture, Marie-Alice
Ujhelyi, Gabriella
Ovesná, Jaroslava
Van Geel, Dirk
De Keersmaecker, Sigrid
Saltykova, Assia
Papazova, Nina
Roosens, Nancy H. C.
author_facet Fraiture, Marie-Alice
Ujhelyi, Gabriella
Ovesná, Jaroslava
Van Geel, Dirk
De Keersmaecker, Sigrid
Saltykova, Assia
Papazova, Nina
Roosens, Nancy H. C.
author_sort Fraiture, Marie-Alice
collection PubMed
description In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette.
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spelling pubmed-65091352019-05-22 MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market Fraiture, Marie-Alice Ujhelyi, Gabriella Ovesná, Jaroslava Van Geel, Dirk De Keersmaecker, Sigrid Saltykova, Assia Papazova, Nina Roosens, Nancy H. C. Sci Rep Article In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the characterization of their transgene flanking regions as well as unnatural associations of elements from their transgenic cassette. Nature Publishing Group UK 2019-05-09 /pmc/articles/PMC6509135/ /pubmed/31073231 http://dx.doi.org/10.1038/s41598-019-43463-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fraiture, Marie-Alice
Ujhelyi, Gabriella
Ovesná, Jaroslava
Van Geel, Dirk
De Keersmaecker, Sigrid
Saltykova, Assia
Papazova, Nina
Roosens, Nancy H. C.
MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
title MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
title_full MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
title_fullStr MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
title_full_unstemmed MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
title_short MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market
title_sort minion sequencing technology to characterize unauthorized gm petunia plants circulating on the european union market
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509135/
https://www.ncbi.nlm.nih.gov/pubmed/31073231
http://dx.doi.org/10.1038/s41598-019-43463-5
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