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Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

The CRISPR-Cas9 system provides the ability to edit, repress, activate, or mark any gene (or DNA element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence on the DNA target. Here we present a new method for small-molecule control of CRISPR-Cas9 function through ins...

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Detalles Bibliográficos
Autores principales:	Kundert, Kale, Lucas, James E., Watters, Kyle E., Fellmann, Christof, Ng, Andrew H., Heineike, Benjamin M., Fitzsimmons, Christina M., Oakes, Benjamin L., Qu, Jiuxin, Prasad, Neha, Rosenberg, Oren S., Savage, David F., El-Samad, Hana, Doudna, Jennifer A., Kortemme, Tanja
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509140/ https://www.ncbi.nlm.nih.gov/pubmed/31073154 http://dx.doi.org/10.1038/s41467-019-09985-2

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