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Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats

BACKGROUND: It is well known that after menopause women are exposed to a greater cardiovascular risk, but the intracellular modifications are not properly described. The sodium/proton exchanger (NHE) and the sodium/bicarbonate cotransporter (NBC) regulate the intracellular pH and, indirectly, the in...

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Autores principales: Ibañez, Alejandro Martín, Espejo, María Sofía, Zavala, Maite Raquel, Villa‐Abrille, María Celeste, Lofeudo, Juan Manuel, Aiello, Ernesto Alejandro, De Giusti, Verónica Celeste
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509710/
https://www.ncbi.nlm.nih.gov/pubmed/30917747
http://dx.doi.org/10.1161/JAHA.118.011066
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author Ibañez, Alejandro Martín
Espejo, María Sofía
Zavala, Maite Raquel
Villa‐Abrille, María Celeste
Lofeudo, Juan Manuel
Aiello, Ernesto Alejandro
De Giusti, Verónica Celeste
author_facet Ibañez, Alejandro Martín
Espejo, María Sofía
Zavala, Maite Raquel
Villa‐Abrille, María Celeste
Lofeudo, Juan Manuel
Aiello, Ernesto Alejandro
De Giusti, Verónica Celeste
author_sort Ibañez, Alejandro Martín
collection PubMed
description BACKGROUND: It is well known that after menopause women are exposed to a greater cardiovascular risk, but the intracellular modifications are not properly described. The sodium/proton exchanger (NHE) and the sodium/bicarbonate cotransporter (NBC) regulate the intracellular pH and, indirectly, the intracellular sodium concentration ([Na(+)]). There are 2 isoforms of NBC in the heart: the electrogenic (1Na(+)/2 [Formula: see text]; NBCe1) and the electroneutral (1Na(+)/1 [Formula: see text]; NBCn1). Because NHE and NBCn1 hyperactivity as well as the NBCe1 decreased activity have been associated with several cardiovascular pathologies, the aim of this study was to investigate the potential alterations of the alkalinizing transporters during the postmenopausal period. METHODS AND RESULTS: Three‐month ovariectomized rats (OVX) were used. The NHE activity and protein expression are significantly increased in OVX. The NBCe1 activity is diminished, and the NBCn1 activity becomes predominant in OVX rats. p‐Akt levels showed a significant diminution in OVX. Finally, NHE activity in platelets from OVX rats is also higher in comparison to sham rats, resulting in a potential biomarker of cardiovascular diseases. CONCLUSIONS: Our results demonstrated for the first time that in the cardiac ventricular myocytes of OVX rats NHE and NBC isoforms are altered, probably because of the decreased level of p‐Akt, compromising the ionic intracellular homeostasis.
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spelling pubmed-65097102019-05-20 Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats Ibañez, Alejandro Martín Espejo, María Sofía Zavala, Maite Raquel Villa‐Abrille, María Celeste Lofeudo, Juan Manuel Aiello, Ernesto Alejandro De Giusti, Verónica Celeste J Am Heart Assoc Original Research BACKGROUND: It is well known that after menopause women are exposed to a greater cardiovascular risk, but the intracellular modifications are not properly described. The sodium/proton exchanger (NHE) and the sodium/bicarbonate cotransporter (NBC) regulate the intracellular pH and, indirectly, the intracellular sodium concentration ([Na(+)]). There are 2 isoforms of NBC in the heart: the electrogenic (1Na(+)/2 [Formula: see text]; NBCe1) and the electroneutral (1Na(+)/1 [Formula: see text]; NBCn1). Because NHE and NBCn1 hyperactivity as well as the NBCe1 decreased activity have been associated with several cardiovascular pathologies, the aim of this study was to investigate the potential alterations of the alkalinizing transporters during the postmenopausal period. METHODS AND RESULTS: Three‐month ovariectomized rats (OVX) were used. The NHE activity and protein expression are significantly increased in OVX. The NBCe1 activity is diminished, and the NBCn1 activity becomes predominant in OVX rats. p‐Akt levels showed a significant diminution in OVX. Finally, NHE activity in platelets from OVX rats is also higher in comparison to sham rats, resulting in a potential biomarker of cardiovascular diseases. CONCLUSIONS: Our results demonstrated for the first time that in the cardiac ventricular myocytes of OVX rats NHE and NBC isoforms are altered, probably because of the decreased level of p‐Akt, compromising the ionic intracellular homeostasis. John Wiley and Sons Inc. 2019-03-28 /pmc/articles/PMC6509710/ /pubmed/30917747 http://dx.doi.org/10.1161/JAHA.118.011066 Text en © 2019 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Ibañez, Alejandro Martín
Espejo, María Sofía
Zavala, Maite Raquel
Villa‐Abrille, María Celeste
Lofeudo, Juan Manuel
Aiello, Ernesto Alejandro
De Giusti, Verónica Celeste
Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_full Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_fullStr Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_full_unstemmed Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_short Regulation of Intracellular pH is Altered in Cardiac Myocytes of Ovariectomized Rats
title_sort regulation of intracellular ph is altered in cardiac myocytes of ovariectomized rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509710/
https://www.ncbi.nlm.nih.gov/pubmed/30917747
http://dx.doi.org/10.1161/JAHA.118.011066
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