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X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation

Radiotherapy-related pain is a common adverse reaction with a high incidence among cancer patients undergoing radiotherapy and remarkably reduces the quality of life. However, the mechanisms of ionizing radiation-induced pain are largely unknown. In this study, mice were treated with 20 Gy X-ray to...

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Autores principales: Cun-Jin, Su, Jian-Hao, Xu, Xu, Liu, Feng-Lun, Zhao, Jie, Pan, Ai-Ming, Shi, Duan-Min, Hu, Yun-Li, Yu, Tong, Liu, Yu-Song, Zhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509987/
https://www.ncbi.nlm.nih.gov/pubmed/31012378
http://dx.doi.org/10.1177/1744806919849201
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author Cun-Jin, Su
Jian-Hao, Xu
Xu, Liu
Feng-Lun, Zhao
Jie, Pan
Ai-Ming, Shi
Duan-Min, Hu
Yun-Li, Yu
Tong, Liu
Yu-Song, Zhang
author_facet Cun-Jin, Su
Jian-Hao, Xu
Xu, Liu
Feng-Lun, Zhao
Jie, Pan
Ai-Ming, Shi
Duan-Min, Hu
Yun-Li, Yu
Tong, Liu
Yu-Song, Zhang
author_sort Cun-Jin, Su
collection PubMed
description Radiotherapy-related pain is a common adverse reaction with a high incidence among cancer patients undergoing radiotherapy and remarkably reduces the quality of life. However, the mechanisms of ionizing radiation-induced pain are largely unknown. In this study, mice were treated with 20 Gy X-ray to establish ionizing radiation-induced pain model. X-ray evoked a prolonged mechanical, heat, and cold allodynia in mice. Transient receptor potential vanilloid 1 and transient receptor potential ankyrin 1 were significantly upregulated in lumbar dorsal root ganglion. The mechanical and heat allodynia could be transiently reverted by intrathecal injection of transient receptor potential vanilloid 1 antagonist capsazepine and transient receptor potential ankyrin 1 antagonist HC-030031. Additionally, the phosphorylated extracellular regulated protein kinases (ERK) and Jun NH2-terminal Kinase (JNK) in pain neural pathway were induced by X-ray treatment. Our findings indicated that activation of transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1 is essential for the development of X-ray-induced allodynia. Furthermore, our findings suggest that targeting on transient receptor potential vanilloid 1 and transient receptor potential ankyrin 1 may be promising prevention strategies for X-ray-induced allodynia in clinical practice.
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spelling pubmed-65099872019-05-17 X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation Cun-Jin, Su Jian-Hao, Xu Xu, Liu Feng-Lun, Zhao Jie, Pan Ai-Ming, Shi Duan-Min, Hu Yun-Li, Yu Tong, Liu Yu-Song, Zhang Mol Pain Research Article Radiotherapy-related pain is a common adverse reaction with a high incidence among cancer patients undergoing radiotherapy and remarkably reduces the quality of life. However, the mechanisms of ionizing radiation-induced pain are largely unknown. In this study, mice were treated with 20 Gy X-ray to establish ionizing radiation-induced pain model. X-ray evoked a prolonged mechanical, heat, and cold allodynia in mice. Transient receptor potential vanilloid 1 and transient receptor potential ankyrin 1 were significantly upregulated in lumbar dorsal root ganglion. The mechanical and heat allodynia could be transiently reverted by intrathecal injection of transient receptor potential vanilloid 1 antagonist capsazepine and transient receptor potential ankyrin 1 antagonist HC-030031. Additionally, the phosphorylated extracellular regulated protein kinases (ERK) and Jun NH2-terminal Kinase (JNK) in pain neural pathway were induced by X-ray treatment. Our findings indicated that activation of transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1 is essential for the development of X-ray-induced allodynia. Furthermore, our findings suggest that targeting on transient receptor potential vanilloid 1 and transient receptor potential ankyrin 1 may be promising prevention strategies for X-ray-induced allodynia in clinical practice. SAGE Publications 2019-05-09 /pmc/articles/PMC6509987/ /pubmed/31012378 http://dx.doi.org/10.1177/1744806919849201 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
Cun-Jin, Su
Jian-Hao, Xu
Xu, Liu
Feng-Lun, Zhao
Jie, Pan
Ai-Ming, Shi
Duan-Min, Hu
Yun-Li, Yu
Tong, Liu
Yu-Song, Zhang
X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation
title X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation
title_full X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation
title_fullStr X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation
title_full_unstemmed X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation
title_short X-ray induces mechanical and heat allodynia in mouse via TRPA1 and TRPV1 activation
title_sort x-ray induces mechanical and heat allodynia in mouse via trpa1 and trpv1 activation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6509987/
https://www.ncbi.nlm.nih.gov/pubmed/31012378
http://dx.doi.org/10.1177/1744806919849201
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