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Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging

The SPB/SFX instrument at the European XFEL provides unique conditions for single-particle imaging (SPI) experiments due to its high brilliance, nano-focus and unique pulse structure. Promising initial results provided by the international LCLS (Linac Coherent Light Source) SPI initiative highlight...

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Autores principales: Uetrecht, Charlotte, Lorenzen, Kristina, Kitel, Matthäus, Heidemann, Johannes, Robinson Spencer, Jesse Huron, Schlüter, Hartmut, Schulz, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510201/
https://www.ncbi.nlm.nih.gov/pubmed/31074428
http://dx.doi.org/10.1107/S1600577519002686
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author Uetrecht, Charlotte
Lorenzen, Kristina
Kitel, Matthäus
Heidemann, Johannes
Robinson Spencer, Jesse Huron
Schlüter, Hartmut
Schulz, Joachim
author_facet Uetrecht, Charlotte
Lorenzen, Kristina
Kitel, Matthäus
Heidemann, Johannes
Robinson Spencer, Jesse Huron
Schlüter, Hartmut
Schulz, Joachim
author_sort Uetrecht, Charlotte
collection PubMed
description The SPB/SFX instrument at the European XFEL provides unique conditions for single-particle imaging (SPI) experiments due to its high brilliance, nano-focus and unique pulse structure. Promising initial results provided by the international LCLS (Linac Coherent Light Source) SPI initiative highlight the potential of SPI. Current available injection methods generally have high sample consumption and do not provide any options for pulsing, selection or orientation of particles, which poses a problem for data evaluation. Aerosol-injector-based sample delivery is the current method of choice for SPI experiments, although, to a lesser extent, electrospray and electrospinning are used. Single particles scatter only a limited number of photons providing a single orientation for data evaluation, hence large datasets are required from particles in multiple orientations in order to reconstruct a structure. Here, a feasibility study demonstrates that nano-electrospray ionization, usually employed in biomolecular mass spectrometry, provides enough ion flux for SPI experiments. A novel instrument setup at the SPB/SFX instrument is proposed, which has the benefit of extremely low background while delivering mass over charge and conformation-selected ions for SPI.
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spelling pubmed-65102012019-06-03 Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging Uetrecht, Charlotte Lorenzen, Kristina Kitel, Matthäus Heidemann, Johannes Robinson Spencer, Jesse Huron Schlüter, Hartmut Schulz, Joachim J Synchrotron Radiat Research Papers The SPB/SFX instrument at the European XFEL provides unique conditions for single-particle imaging (SPI) experiments due to its high brilliance, nano-focus and unique pulse structure. Promising initial results provided by the international LCLS (Linac Coherent Light Source) SPI initiative highlight the potential of SPI. Current available injection methods generally have high sample consumption and do not provide any options for pulsing, selection or orientation of particles, which poses a problem for data evaluation. Aerosol-injector-based sample delivery is the current method of choice for SPI experiments, although, to a lesser extent, electrospray and electrospinning are used. Single particles scatter only a limited number of photons providing a single orientation for data evaluation, hence large datasets are required from particles in multiple orientations in order to reconstruct a structure. Here, a feasibility study demonstrates that nano-electrospray ionization, usually employed in biomolecular mass spectrometry, provides enough ion flux for SPI experiments. A novel instrument setup at the SPB/SFX instrument is proposed, which has the benefit of extremely low background while delivering mass over charge and conformation-selected ions for SPI. International Union of Crystallography 2019-04-11 /pmc/articles/PMC6510201/ /pubmed/31074428 http://dx.doi.org/10.1107/S1600577519002686 Text en © Charlotte Uetrecht et al. 2019 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Uetrecht, Charlotte
Lorenzen, Kristina
Kitel, Matthäus
Heidemann, Johannes
Robinson Spencer, Jesse Huron
Schlüter, Hartmut
Schulz, Joachim
Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
title Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
title_full Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
title_fullStr Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
title_full_unstemmed Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
title_short Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
title_sort native mass spectrometry provides sufficient ion flux for xfel single-particle imaging
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6510201/
https://www.ncbi.nlm.nih.gov/pubmed/31074428
http://dx.doi.org/10.1107/S1600577519002686
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