A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples

Norovirus is a highly prevalent pathogen that can infect a wide range of host species. Thus far, there have only been two reports of norovirus infection in rats. Diagnostic assays for the detection of norovirus are well established, but a specific molecular assay for the diagnosis of norovirus infec...

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Autores principales: Moreira, Josélia Cristina de Oliveira, Rodrigues, Daniele Masselli, Gilioli, Rovilson, Costa, Fernando Ferreira, Albuquerque, Dulcinéia Martins
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2018
Materias:
Original
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511519/
https://www.ncbi.nlm.nih.gov/pubmed/30531093
http://dx.doi.org/10.1538/expanim.18-0049
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author Moreira, Josélia Cristina de Oliveira
Rodrigues, Daniele Masselli
Gilioli, Rovilson
Costa, Fernando Ferreira
Albuquerque, Dulcinéia Martins
author_facet Moreira, Josélia Cristina de Oliveira
Rodrigues, Daniele Masselli
Gilioli, Rovilson
Costa, Fernando Ferreira
Albuquerque, Dulcinéia Martins
author_sort Moreira, Josélia Cristina de Oliveira
collection PubMed
description Norovirus is a highly prevalent pathogen that can infect a wide range of host species. Thus far, there have only been two reports of norovirus infection in rats. Diagnostic assays for the detection of norovirus are well established, but a specific molecular assay for the diagnosis of norovirus infection in laboratory rats has not yet been reported. In this study, we describe the development of a sensitive, semi-nested RT-PCR assay for detection of norovirus in fecal samples from Rattus norvegicus, reared in animal facilities under different sanitary barrier conditions. Additionally, we describe the first report of the presence of norovirus in rat colonies from Brazilian animal facilities.
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spelling pubmed-65115192019-05-15 A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples Moreira, Josélia Cristina de Oliveira Rodrigues, Daniele Masselli Gilioli, Rovilson Costa, Fernando Ferreira Albuquerque, Dulcinéia Martins Exp Anim Original Norovirus is a highly prevalent pathogen that can infect a wide range of host species. Thus far, there have only been two reports of norovirus infection in rats. Diagnostic assays for the detection of norovirus are well established, but a specific molecular assay for the diagnosis of norovirus infection in laboratory rats has not yet been reported. In this study, we describe the development of a sensitive, semi-nested RT-PCR assay for detection of norovirus in fecal samples from Rattus norvegicus, reared in animal facilities under different sanitary barrier conditions. Additionally, we describe the first report of the presence of norovirus in rat colonies from Brazilian animal facilities. Japanese Association for Laboratory Animal Science 2018-12-07 2019 /pmc/articles/PMC6511519/ /pubmed/30531093 http://dx.doi.org/10.1538/expanim.18-0049 Text en ©2019 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original
Moreira, Josélia Cristina de Oliveira
Rodrigues, Daniele Masselli
Gilioli, Rovilson
Costa, Fernando Ferreira
Albuquerque, Dulcinéia Martins
A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples
title A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples
title_full A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples
title_fullStr A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples
title_full_unstemmed A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples
title_short A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples
title_sort semi-nested rt-pcr assay for detection of norovirus in rat fecal samples
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511519/
https://www.ncbi.nlm.nih.gov/pubmed/30531093
http://dx.doi.org/10.1538/expanim.18-0049
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