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Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice

Pancreatic acinar cell vacuolation is spontaneously observed in mice; however, the lesion is rare and has not been well documented. Herein, we present a detailed pathological examination of this lesion. Vacuoles in pancreatic acinar cells were present in 2/15 X gene knockout mice with a C57BL/6J mou...

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Autores principales: Yasuno, Kyohei, Igura, Saori, Yamaguchi, Yuko, Imaoka, Masako, Kai, Kiyonori, Mori, Kazuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Toxicologic Pathology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511541/
https://www.ncbi.nlm.nih.gov/pubmed/31092977
http://dx.doi.org/10.1293/tox.2018-0065
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author Yasuno, Kyohei
Igura, Saori
Yamaguchi, Yuko
Imaoka, Masako
Kai, Kiyonori
Mori, Kazuhiko
author_facet Yasuno, Kyohei
Igura, Saori
Yamaguchi, Yuko
Imaoka, Masako
Kai, Kiyonori
Mori, Kazuhiko
author_sort Yasuno, Kyohei
collection PubMed
description Pancreatic acinar cell vacuolation is spontaneously observed in mice; however, the lesion is rare and has not been well documented. Herein, we present a detailed pathological examination of this lesion. Vacuoles in pancreatic acinar cells were present in 2/15 X gene knockout mice with a C57BL/6J mouse background, 4/298 ICR(CD-1) mice, 1/110 B6C3F1 mice, and 3/399 CByB6F1-Tg(HRAS)2Jic mice. The vacuoles were usually observed in a unit of the acinus, and the lesions were spread throughout the pancreas. These vacuoles contained weakly basophilic material that was positive for the periodic acid-Schiff reaction. Immunohistochemically, the vacuoles were positive for calreticulin antibody. Electron microscopy revealed globular dilatation of the rough endoplasmic reticulum (rER). According to these findings, vacuolation of pancreatic acinar cells is caused by the accumulation of misfolded proteins and enlargement of the rER.
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spelling pubmed-65115412019-05-15 Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice Yasuno, Kyohei Igura, Saori Yamaguchi, Yuko Imaoka, Masako Kai, Kiyonori Mori, Kazuhiko J Toxicol Pathol Case Report Pancreatic acinar cell vacuolation is spontaneously observed in mice; however, the lesion is rare and has not been well documented. Herein, we present a detailed pathological examination of this lesion. Vacuoles in pancreatic acinar cells were present in 2/15 X gene knockout mice with a C57BL/6J mouse background, 4/298 ICR(CD-1) mice, 1/110 B6C3F1 mice, and 3/399 CByB6F1-Tg(HRAS)2Jic mice. The vacuoles were usually observed in a unit of the acinus, and the lesions were spread throughout the pancreas. These vacuoles contained weakly basophilic material that was positive for the periodic acid-Schiff reaction. Immunohistochemically, the vacuoles were positive for calreticulin antibody. Electron microscopy revealed globular dilatation of the rough endoplasmic reticulum (rER). According to these findings, vacuolation of pancreatic acinar cells is caused by the accumulation of misfolded proteins and enlargement of the rER. Japanese Society of Toxicologic Pathology 2019-02-07 2019-04 /pmc/articles/PMC6511541/ /pubmed/31092977 http://dx.doi.org/10.1293/tox.2018-0065 Text en ©2019 The Japanese Society of Toxicologic Pathology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Case Report
Yasuno, Kyohei
Igura, Saori
Yamaguchi, Yuko
Imaoka, Masako
Kai, Kiyonori
Mori, Kazuhiko
Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice
title Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice
title_full Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice
title_fullStr Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice
title_full_unstemmed Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice
title_short Pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice
title_sort pathological examination of spontaneous vacuolation of pancreatic acinar cells in mice
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511541/
https://www.ncbi.nlm.nih.gov/pubmed/31092977
http://dx.doi.org/10.1293/tox.2018-0065
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