Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran

BACKGROUND: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically. METHODS: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghae...

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Autores principales: BERENJI, Fariba, SHAMSIAN, Seyed Aliakbar, NOURI DALOEE, Marziyeh, FATTAHI MASOOM, Seyed Hossein, MOGHADDAS, Elham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511601/
https://www.ncbi.nlm.nih.gov/pubmed/31123468
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author BERENJI, Fariba
SHAMSIAN, Seyed Aliakbar
NOURI DALOEE, Marziyeh
FATTAHI MASOOM, Seyed Hossein
MOGHADDAS, Elham
author_facet BERENJI, Fariba
SHAMSIAN, Seyed Aliakbar
NOURI DALOEE, Marziyeh
FATTAHI MASOOM, Seyed Hossein
MOGHADDAS, Elham
author_sort BERENJI, Fariba
collection PubMed
description BACKGROUND: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically. METHODS: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015–2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1). RESULTS: Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified. CONCLUSION: Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.
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spelling pubmed-65116012019-05-23 Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran BERENJI, Fariba SHAMSIAN, Seyed Aliakbar NOURI DALOEE, Marziyeh FATTAHI MASOOM, Seyed Hossein MOGHADDAS, Elham Iran J Parasitol Original Article BACKGROUND: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically. METHODS: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015–2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1). RESULTS: Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified. CONCLUSION: Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region. Tehran University of Medical Sciences 2019 /pmc/articles/PMC6511601/ /pubmed/31123468 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
BERENJI, Fariba
SHAMSIAN, Seyed Aliakbar
NOURI DALOEE, Marziyeh
FATTAHI MASOOM, Seyed Hossein
MOGHADDAS, Elham
Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
title Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
title_full Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
title_fullStr Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
title_full_unstemmed Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
title_short Genotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
title_sort genotyping of echinococcus granulosus isolates from human in khorasan province, north-eastern iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6511601/
https://www.ncbi.nlm.nih.gov/pubmed/31123468
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