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Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice

Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to treat many diseases including hypertension. This study investigated whether oral administration of the ethyl acetate fraction of Lannea microcarpa trunk barks (LMAE) corrects angiotensin (Ang) II-induce...

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Autores principales: Nitiéma, Mathieu, Soleti, Raffaella, Koffi, Camille, Belemnaba, Lazare, Mallegol, Patricia, Ouédraogo, Noufou, Kini, Félix Bondo, Ouédraogo, Sylvin, Guissou, Innocent Pierre, Andriantsitohaina, Ramaroson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6512010/
https://www.ncbi.nlm.nih.gov/pubmed/31183001
http://dx.doi.org/10.1155/2019/9464608
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author Nitiéma, Mathieu
Soleti, Raffaella
Koffi, Camille
Belemnaba, Lazare
Mallegol, Patricia
Ouédraogo, Noufou
Kini, Félix Bondo
Ouédraogo, Sylvin
Guissou, Innocent Pierre
Andriantsitohaina, Ramaroson
author_facet Nitiéma, Mathieu
Soleti, Raffaella
Koffi, Camille
Belemnaba, Lazare
Mallegol, Patricia
Ouédraogo, Noufou
Kini, Félix Bondo
Ouédraogo, Sylvin
Guissou, Innocent Pierre
Andriantsitohaina, Ramaroson
author_sort Nitiéma, Mathieu
collection PubMed
description Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to treat many diseases including hypertension. This study investigated whether oral administration of the ethyl acetate fraction of Lannea microcarpa trunk barks (LMAE) corrects angiotensin (Ang) II-induced hypertension in mice. Its effects on vascular function were specifically investigated. Experiments explored hemodynamic and echocardiographic parameters in vivo and vascular reactivity to acetylcholine (ACh) and CaCl(2) ex vivo on isolated aortas. Mice received LMAE for 3 weeks (50 mg/kg/day) by oral gavage. In the last two weeks of treatment, mice were implanted with osmotic minipumps delivering NaCl (0.9%) or Ang II (0.5 mg/kg/day). LMAE completely prevented the increase in systolic and diastolic blood pressure induced by Ang II. Echocardiographic and kidney parameters were not affected by the different conditions. LMAE abrogated Ang II-induced impairment of ACh-induced relaxation without affecting that of sodium nitroprusside. LMAE also completely prevented CaCl(2)-induced contraction in KCl-exposed aorta ex vivo. The extract alone did not modify superoxide (O(2) (−)) and nitric oxide (NO(·)) production in femoral arteries from control mice but significantly limited Ang II-induced O(2) (−) production. These effects were associated with reduced expression of inducible isoform of cyclooxygenase- (COX-) 2 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase isoform NOX-2 in aortas. Finally, phytochemical analysis showed that LMAE contains sterols, triterpenes, coumarins, and anthraquinone. These results showed that LMAE prevents Ang II-induced hypertension and vascular dysfunction through a reduction of oxidative stress linked to COX-2 and NOX-2 pathway and inhibition of calcium entry. This study provides pharmacological basis of the empirical use of Lannea microcarpa trunk bark extract against hypertension.
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spelling pubmed-65120102019-06-10 Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice Nitiéma, Mathieu Soleti, Raffaella Koffi, Camille Belemnaba, Lazare Mallegol, Patricia Ouédraogo, Noufou Kini, Félix Bondo Ouédraogo, Sylvin Guissou, Innocent Pierre Andriantsitohaina, Ramaroson Oxid Med Cell Longev Research Article Traditional remedies prepared from Lannea microcarpa leaves, barks, roots, and fruits are used to treat many diseases including hypertension. This study investigated whether oral administration of the ethyl acetate fraction of Lannea microcarpa trunk barks (LMAE) corrects angiotensin (Ang) II-induced hypertension in mice. Its effects on vascular function were specifically investigated. Experiments explored hemodynamic and echocardiographic parameters in vivo and vascular reactivity to acetylcholine (ACh) and CaCl(2) ex vivo on isolated aortas. Mice received LMAE for 3 weeks (50 mg/kg/day) by oral gavage. In the last two weeks of treatment, mice were implanted with osmotic minipumps delivering NaCl (0.9%) or Ang II (0.5 mg/kg/day). LMAE completely prevented the increase in systolic and diastolic blood pressure induced by Ang II. Echocardiographic and kidney parameters were not affected by the different conditions. LMAE abrogated Ang II-induced impairment of ACh-induced relaxation without affecting that of sodium nitroprusside. LMAE also completely prevented CaCl(2)-induced contraction in KCl-exposed aorta ex vivo. The extract alone did not modify superoxide (O(2) (−)) and nitric oxide (NO(·)) production in femoral arteries from control mice but significantly limited Ang II-induced O(2) (−) production. These effects were associated with reduced expression of inducible isoform of cyclooxygenase- (COX-) 2 and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase isoform NOX-2 in aortas. Finally, phytochemical analysis showed that LMAE contains sterols, triterpenes, coumarins, and anthraquinone. These results showed that LMAE prevents Ang II-induced hypertension and vascular dysfunction through a reduction of oxidative stress linked to COX-2 and NOX-2 pathway and inhibition of calcium entry. This study provides pharmacological basis of the empirical use of Lannea microcarpa trunk bark extract against hypertension. Hindawi 2019-04-28 /pmc/articles/PMC6512010/ /pubmed/31183001 http://dx.doi.org/10.1155/2019/9464608 Text en Copyright © 2019 Mathieu Nitiéma et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nitiéma, Mathieu
Soleti, Raffaella
Koffi, Camille
Belemnaba, Lazare
Mallegol, Patricia
Ouédraogo, Noufou
Kini, Félix Bondo
Ouédraogo, Sylvin
Guissou, Innocent Pierre
Andriantsitohaina, Ramaroson
Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice
title Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice
title_full Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice
title_fullStr Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice
title_full_unstemmed Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice
title_short Ethyl Acetate Fraction of Lannea microcarpa Engl. and K. Krause (Anacardiaceae) Trunk Barks Corrects Angiotensin II-Induced Hypertension and Endothelial Dysfunction in Mice
title_sort ethyl acetate fraction of lannea microcarpa engl. and k. krause (anacardiaceae) trunk barks corrects angiotensin ii-induced hypertension and endothelial dysfunction in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6512010/
https://www.ncbi.nlm.nih.gov/pubmed/31183001
http://dx.doi.org/10.1155/2019/9464608
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