Cargando…

Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis

Lumbar spinal stenosis (LSS) is a major cause of chronic low back pain; however, only a few therapies which have been used in clinics still have limited effects on functional recovery. SHINBARO2 is a refined traditional formulation for inflamed lesions and relieve pain of muscular skeletal disease....

Descripción completa

Detalles Bibliográficos
Autores principales: Park, So Hyun, Hong, Ji-Young, Kim, Won Kyung, Shin, Joon-Shik, Lee, Jinho, Ha, In-Hyuk, Chung, Hwa-Jin, Lee, Sang Kook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6512060/
https://www.ncbi.nlm.nih.gov/pubmed/31182933
http://dx.doi.org/10.1155/2019/7651470
_version_ 1783417640594702336
author Park, So Hyun
Hong, Ji-Young
Kim, Won Kyung
Shin, Joon-Shik
Lee, Jinho
Ha, In-Hyuk
Chung, Hwa-Jin
Lee, Sang Kook
author_facet Park, So Hyun
Hong, Ji-Young
Kim, Won Kyung
Shin, Joon-Shik
Lee, Jinho
Ha, In-Hyuk
Chung, Hwa-Jin
Lee, Sang Kook
author_sort Park, So Hyun
collection PubMed
description Lumbar spinal stenosis (LSS) is a major cause of chronic low back pain; however, only a few therapies which have been used in clinics still have limited effects on functional recovery. SHINBARO2 is a refined traditional formulation for inflamed lesions and relieve pain of muscular skeletal disease. This study aimed at investigating the effects of SHINBARO2 on LSS and at determining its underlying molecular mechanism in rat models. The LSS rat models were set up by surgical operations in 6-week-old male Sprague-Dawley rats. SHINBARO2 was orally or intraperitoneally administered for 14 days. The motor and sensory ability of rats were evaluated using the activity cage and hot plate method. On the termination day, total vertebrae including the disc and spinal cord were excised for ex vivo study. SHINBARO2 improved locomotor functions and pain sensitivity in LSS rat models. Mechanism study suggested that SHINBARO2 inhibited the production of nitric oxide and prostaglandin E(2) in tissues from LSS-induced rats. SHINBARO2 also suppressed the expression of proinflammatory cytokines including tumor necrosis factor-α and interleukin-1β. The activation of NF-κB by LSS surgery was effectively reduced by SHINBARO2, which coincided with the inhibition of IκB degradation. In addition, brain-derived neurotrophic factor (BDNF), a potent promoter of neurite growth, and its downstream ERK signaling were also regulated by SHINBARO2. These findings suggest that the effect of SHINBARO2 might be associated in part with the anti-inflammation and pain control in LSS rat models.
format Online
Article
Text
id pubmed-6512060
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-65120602019-06-10 Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis Park, So Hyun Hong, Ji-Young Kim, Won Kyung Shin, Joon-Shik Lee, Jinho Ha, In-Hyuk Chung, Hwa-Jin Lee, Sang Kook Mediators Inflamm Research Article Lumbar spinal stenosis (LSS) is a major cause of chronic low back pain; however, only a few therapies which have been used in clinics still have limited effects on functional recovery. SHINBARO2 is a refined traditional formulation for inflamed lesions and relieve pain of muscular skeletal disease. This study aimed at investigating the effects of SHINBARO2 on LSS and at determining its underlying molecular mechanism in rat models. The LSS rat models were set up by surgical operations in 6-week-old male Sprague-Dawley rats. SHINBARO2 was orally or intraperitoneally administered for 14 days. The motor and sensory ability of rats were evaluated using the activity cage and hot plate method. On the termination day, total vertebrae including the disc and spinal cord were excised for ex vivo study. SHINBARO2 improved locomotor functions and pain sensitivity in LSS rat models. Mechanism study suggested that SHINBARO2 inhibited the production of nitric oxide and prostaglandin E(2) in tissues from LSS-induced rats. SHINBARO2 also suppressed the expression of proinflammatory cytokines including tumor necrosis factor-α and interleukin-1β. The activation of NF-κB by LSS surgery was effectively reduced by SHINBARO2, which coincided with the inhibition of IκB degradation. In addition, brain-derived neurotrophic factor (BDNF), a potent promoter of neurite growth, and its downstream ERK signaling were also regulated by SHINBARO2. These findings suggest that the effect of SHINBARO2 might be associated in part with the anti-inflammation and pain control in LSS rat models. Hindawi 2019-04-28 /pmc/articles/PMC6512060/ /pubmed/31182933 http://dx.doi.org/10.1155/2019/7651470 Text en Copyright © 2019 So Hyun Park et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Park, So Hyun
Hong, Ji-Young
Kim, Won Kyung
Shin, Joon-Shik
Lee, Jinho
Ha, In-Hyuk
Chung, Hwa-Jin
Lee, Sang Kook
Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis
title Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis
title_full Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis
title_fullStr Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis
title_full_unstemmed Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis
title_short Effects of SHINBARO2 on Rat Models of Lumbar Spinal Stenosis
title_sort effects of shinbaro2 on rat models of lumbar spinal stenosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6512060/
https://www.ncbi.nlm.nih.gov/pubmed/31182933
http://dx.doi.org/10.1155/2019/7651470
work_keys_str_mv AT parksohyun effectsofshinbaro2onratmodelsoflumbarspinalstenosis
AT hongjiyoung effectsofshinbaro2onratmodelsoflumbarspinalstenosis
AT kimwonkyung effectsofshinbaro2onratmodelsoflumbarspinalstenosis
AT shinjoonshik effectsofshinbaro2onratmodelsoflumbarspinalstenosis
AT leejinho effectsofshinbaro2onratmodelsoflumbarspinalstenosis
AT hainhyuk effectsofshinbaro2onratmodelsoflumbarspinalstenosis
AT chunghwajin effectsofshinbaro2onratmodelsoflumbarspinalstenosis
AT leesangkook effectsofshinbaro2onratmodelsoflumbarspinalstenosis